Nuclear acid probe marked with quantum-dots composite sphere and preparation method and application thereof

Abstract

The invention discloses a nuclear acid probe marked with quantum-dots composite sphere and a preparation method and application thereof. Nuclear acid probes with different sequences are marked with quantum-dots composite sphere with different emission spectrum characteristics; the quantum-dots composite sphere comprises a sphere and a quantum-dots composite contained in the sphere, the quantum-dots composite is composed of randomly selected m types of quantum-dots with different emission spectrum characteristics from n types thereof, n and m are both positive whole numbers and m is less than or equal to n; the preparation method of the nuclear acid probe comprises the following steps: preparing numerous probes with different sequences and marking the probes with X; preparing numerous quantum-dots composite sphere with different emission spectrum characteristics and marking the sphere with Y; integrating probes marked with X and quantum-dots composite sphere marked with Y through specificity of X and Y and carrying out coupling on the probes and sphere; applied to multicolor fluorescence in situ hybridization, spectral karyotype analysis and chromosome karyotype analysis, the probeof the invention can improve sensitivity, repetitiveness and specificity of detection, simplify detection device, increase detection speed and prevent false positive result from occurring.

Description

technical field [0001] The invention relates to the fields of molecular biology and cytogenetics, in particular to a quantum dot combined microsphere labeled nucleic acid probe and a preparation method and application thereof. Background technique [0002] Chromosomes of different species have their own specific morphological and structural characteristics (including chromosome length, centromere position, arm ratio, satellite size, etc.), and this morphological and structural characteristics are relatively stable. Therefore, karyotype analysis is an important content of genetic research, and it is also of great scientific significance for the study of the occurrence, development, and metastasis mechanisms of human chromosomal diseases and malignant tumors. [0003] The traditional method of chromosome karyotype analysis is mainly the chromosome G banding method, which has the advantages of simple operation and low cost, and can accurately distinguish the deletion, duplicati...

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Problems solved by technology

[0006] In view of this, one of the objectives of the present invention is to provide a quantum dot combined microsphere-labeled nucleic acid probe, which can overcome the weak fluorescence intensity, easy photobleaching, and different fluorescent dye-labeled nucleic acid probes existing in the existing fluorescent dye-labeled nucleic acid probes. Nucleic acid probes need to be excited at different wavelengths and have large interference between spectra, etc.

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