Analysis method of sharp leaf CinnamomumKanehirae Hay karyotype based on root tips

A technology of chromosome karyotype and analysis method, which is applied in the field of root tip-based analysis of the chromosome karyotype of Cinnamomum chinensis to achieve the effect of easy preservation
CN110220904AInactive Publication Date: 2019-09-10广州甘蔗糖业研究所湛江甘蔗研究中心
7 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
广州甘蔗糖业研究所湛江甘蔗研究中心
Publication Date
2019-09-10
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an analysis method of sharp leaf CinnamomumKanehirae Hay karyotype based on root tips. The method comprises the following steps: collecting a robust, white and tender root tipof a tissue culture seedling of CinnamomumKanehirae Hay in a sunny morning to serve as a test material, pretreating, fixing and storing the test material, performing fixation, anterior hyposmosis, enzymolysis, post hyposmosis and smearing by using an enzymolysis wall removal hyposmosis method to obtain a better chromosome specimen, wherein the enzymolysis is performed by using a mixture of 4% cellulase and 5% pectinase, and performing Giemsa staining and microscopic examination. The method is simple and easy to operate, is suitable for the analysis method of sharp leaf CinnamomumKanehirae Haykaryotype with in-situ PCR, FISH operations, and provides a technical basis for the cytological study of the CinnamomumKanehirae Hay. The karyotype analysis method and the karyotype of the sharp leafCinnamomumKanehirae Hay are reported in the invention for the first time, which indicates that the CinnamomumKanehirae Hay has 24 chromosomes, the karyotype formula is 2n=2x=24=22m+2sm, the asymmetrycoefficient of the karyotype is 55.81%, and the karyotype belongs to a type 2B and belongs to relatively primitive species. A certain theoretical basis is provided for the origin, evolution and genetic breeding of the sharp leaf CinnamomumKanehirae Hay.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a chromosome karyotype analysis method, in particular to a root tip-based chromosome karyotype analysis method of Cinnamomum camphorii, belonging to the field of tree cytology research technology. Background technique

[0002] Cinnamomum Kanehirae Hay belongs to the genus Lauraceae, also known as black camphor, which is a native evergreen broad-leaved tree species in Taiwan, China. Cinnamomum camphora has high economic value. The whole plant of roots, stems and leaves can be used to extract the essential oil of Cinnamomum camphora. Wood is the best material for carving furniture. In addition, Antrodia camphorata is the only host of Antrodia camphorata. Antrodia camphorata is known as the "king of medicinal mushrooms" in medicine. It is often used to sober up and relieve fatigue, and has immune regulation and liver protection effects.

[0003] The research on Cinnamomum camphora started late, and the research direction was sing...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More