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Frankliniella occidentalis arrhenotoky inbreeding population establishing method

A technology of inbreeding and parthenogenesis, which is applied in the field of insect breeding and population establishment, can solve the problems of high mortality rate, weak vitality, and fast movement of small animals in single-head feeding, and achieve the goal of avoiding escape or death and ensuring survival rate Effect

Active Publication Date: 2015-07-22
QINGDAO AGRI UNIV
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for establishing an androgenetic inbreeding population of Western flower thrips, which overcomes the shortcomings of western flower thrips individual small movement, weak vitality, and high mortality rate of single feeding

Method used

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  • Frankliniella occidentalis arrhenotoky inbreeding population establishing method

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Embodiment 1

[0031] 1. Preparation of materials: Cut the 200-mesh gauze into two sizes of 2cm×2cm and 3cm×3cm for use; burn the awl red on the alcohol lamp, and cut out a hole with a diameter of 3mm in the cap of the 1.5ml centrifuge tube. Burn the wire ring red, and cut out a hole with a diameter of 8mm in the cap of the 10ml centrifuge tube. All centrifuge tubes must be sterilized at high temperature before use; cut the filter paper into bottom x height of 1.5cm x 0.5cm and 3cm x 1cm Two specifications of triangular filter paper are used for spare; buy fresh purple cabbage to remove the thin leaves, cut the thick part of the leaf into small pieces with a volume of about 3mm×3mm×10mm, and put the cabbage pieces with uniform and regular shape into a 10ml tube ; Light incubator, environmental conditions for raising Thrips occidentalis: temperature 25°C, humidity 60%-80%, photoperiod L:D=14:8.

[0032] 2. Select the first eclosion females

[0033] Put triangular filter paper and small cabba...

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Abstract

The invention discloses a frankliniella occidentalis arrhenotoky inbreeding population establishing method. According to the method, frankliniella occidentalis artificially bred is transferred into a centrifugal tube for single parthenogenetic breeding to establish an inbreeding population by controlling temperature, moisture and illumination manually. The method is characterized in that the frankliniella occidentalis arrhenotoky inbreeding population can be established, and obtained male offspring and multiple continuous generations of male and female offspring can be applied to karyotype analysis, microsatellite analysis and other experiments. The method has great significance for further learning the frankliniella occidentalis arrhenotoky features and establishing the inbreeding population and can further promote the analysis of genetic structure and sex determination mode.

Description

technical field [0001] The invention relates to an insect breeding and population establishment technology, in particular to a method for establishing an androgenetic inbreeding population of thrips occidentalis. Background technique [0002] The adult stage of Thrips occidentalis is significantly longer than the immature stage, and the life span of females is significantly longer than that of males, which provides good innate conditions for studying its sex determination mechanism through the establishment of inbreeding populations. Taking the backcross breeding of Western flower Thrips as the starting point to establish androgenetic inbreeding population of Western flower Thrips, with the help of karyotype analysis, microsatellite analysis, identification of differentially expressed proteins, bioinformatics analysis and RNAi, etc. Systematic detection and comparative analysis were carried out at each stage of the development of inbred populations of Thrips floridae, to det...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 张彬郑长英臧萌孙启霞姜培茹李洪刚
Owner QINGDAO AGRI UNIV
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