76 results about "Western flower thrips" patented technology

The invention relates to the field of plant protection and the technology of insect cultivation, and in particular relates to a method for breeding frankliniella occidentalis by using water planting soya bean seedlings as fodder. According to the conventional method, kidney beans are used as the fodder, while the soya bean seedlings are used for feeding the frankliniella occidentalis according to the method, and therefore, the defect of feeding the frankliniella occidentalis caused by the problem of the fodder per se is changed. As the water planting soya bean seedlings have a short growth cycle, a low production environment requirement and low production cost and can be supplied throughout the year, according to the method for breeding the frankliniella occidentalis, the fodder basis for feeding the frankliniella occidentalis in a large scale can be provided and an excellent feeding effect can be realized.

The invention relates to a Beaueria bassaria (Balsamo) Vuillemin bacterial strain GZGY-1-3, a culture method of the bacterial strain, spore powder prepared from the bacterial strain and application of the spore powder in preparing a biocontrol agent, wherein the biocontrol agent can be used for preventing and controlling bemisia tabaci, frankliniella occidentalis, green onion aphidand/or green peach aphid. The biocontrol agent can be used for preventing and controlling insects under a high temperature environment, and is good in prevention and control effect with field control efficiency over 75%.

The invention relates to a method for artificially and massively feeding natural enemy staphylinid. The method is carried out in an environment with temperature ranging from 18 DEG C to 32 DEG C and relative humidity ranging from 65% to 95%, and tyrophagus putrescentiae and carpoglyphus lactis are used for artificially feeding massive Atheta coriaria and Oligota flavicornis. Feed base materials respectively comprises 35% -65% and 25% -50% of water. Feeding ratio between prey acarus and the natural enemy staphylinid is 50:1-1000:1. The feeding lasts for 20-50 days. When population density of the natural enemy staphylinid is higher than or equal to 400 pcs./liter, the natural enemy staphylinid can be packaged into finished products or be put into different containers for feeding. The feeding method is simple, highly effective and of low cost and can provide a wide range of natural enemy staphylinid sources for preventing and killing fungus gnat, ephydrid, frankliniella occidentalis, springtails, nematodes, tetranychus truncatus ehara and other pests for purpose of generalizing the biological prevention technologies of China, therefore, the use of chemical pesticides can be reduced, the eco-environment is improved and the international competiveness of agricultural products can be improved.

Aiming at the defects of conidiospores as propagula of an insecticide of insect pathogenic fungi, the invention provides a technology of liquid culture of entomogenous fungus sclerotia, and the entomogenous fungus can be beauveria bassiana. The liquid culture method of entomogenous fungus sclerotia provided by the invention is required to control the liquid culture components and the culture conditions within a certain range. The cultured sclerotia can be stored for a long time after vacuum drying, and can still germinate mycelia and pathogenic conidiospores under suitable conditions. The micro-sclerotia prepared by drying have maintained activity, can germinate mycelia and spores after rehydration, and increase the potential of beauveria bassiana as a biocontrol factor for western flowerthrips in a soil-dwelling phase. The technology has low cost, long storage period of sclerotia, and is quite suitable for popularization.

An antiviral composition and a method of treating viral conditions comprising colloidal silver and an antiviral natural herb or a natural herb from the Rutaceae family, such as Ruta graveolens, Myrtaceae family, such as Melaleuca alternifolia, Cupressaceae family, such as Thuja occidentalis, and the Moraceae family, such as unripe fig, or any combination thereof.

The invention discloses a prevention and control method for coriander frankliniella occidentalis. The method comprises the steps that 1) preparation is completed before planting; 2) a silicon fertilizer is applied, and the applying method is that in the early stage of coriander planting, the silicon fertilizer with the concentration of 2-10 kilograms per mu is used for flushing, applying and dropirrigation; 3) a nitrogenous fertilizer is applied, the nitrogenous fertilizer is one or more of 1.0%-3.0% of urea solution, 0.5%-1.5% of ammonium bicarbonate solution, 0.3%-1.0% of sal volatile solution and 0.1%-1.0% of hartshorn, and the fertilizer is sprayed to portions outside roots; 4) artificial isolation is adopted. The method is used for preventing and controlling the frankliniella occidentalis, pesticide residues on coriander are reduced, coriander quality is improved, safety threat of pesticide to creatures is reduced, and chemical pesticide resistance generation of the frankliniellaoccidentalis is reduced.

The invention relates to a primer and a method for authenticating frankliniella occidentalis by using expressed sequence tag (EST) microsatellite markers. The method comprises the following steps of: (1) extracting the genomic DNA of thrips; (2) performing polymerase chain reaction (PCR) on the genomic DNA of thrips, which serves as a template; and (3) performing sepharose gel electrophoretic analysis on an enzyme-digested product prepared in the step (2). By the authentication method, simple and stable molecule markers are supplied to screening of frankliniella occidentalis and other three types of thrips and a foundation is laid for the future research on the population dynamic authentication, the biology and an invasive mechanism of frankliniella occidentalis.

The invention discloses a frankliniella occidentalis arrhenotoky inbreeding population establishing method. According to the method, frankliniella occidentalis artificially bred is transferred into a centrifugal tube for single parthenogenetic breeding to establish an inbreeding population by controlling temperature, moisture and illumination manually. The method is characterized in that the frankliniella occidentalis arrhenotoky inbreeding population can be established, and obtained male offspring and multiple continuous generations of male and female offspring can be applied to karyotype analysis, microsatellite analysis and other experiments. The method has great significance for further learning the frankliniella occidentalis arrhenotoky features and establishing the inbreeding population and can further promote the analysis of genetic structure and sex determination mode.

The invention relates to the field of molecular biology, in particular to a specific COI primer, a detection method and a reagent kit of Frankliniella occidentalis. The nucleotide sequences of the specific COI primer of the Frankliniella occidentalis of the invention are shown as SEQ IDNo: 1 and 2. According to the specific mitochondrion DNA sequence of the Frankliniella occidentalis, the invention designs a pair of specific primers which only have amplification capability on the Frankliniella occidentalis. The invention is the supplement and improvement on the RFLP technology and the mtDNA COI technology detection method of the Frankliniella occidentalis.

The invention discloses a repellent used for frankliniella occidentalis and an application of active ingredients of the repellent, and relates to the technical field of agriculture. The active ingredients of the repellent used for the frankliniella occidentalis comprise at least one selected from the group consisting of alpha-pinene, cineole, camphene, (-)-borneol, beta-pinene, D(+)-camphor, beta-myrcene and bornyl acetate. The active ingredients of the repellent provided by the invention are green and pollution-free chemicals, and have an obvious repellent effect on the frankliniella occidentalis.

The invention discloses a breeding method and device of orius similis. The device is characterized in that: kidney bean seeds are laid at the bottom of a glass container; tender jasminum nudiflorum shoots are arranged on the inner wall of the glass container; the opening of the glass container is sealed with a piece of gauze; a nutrition supplementation bar is hung below the gauze. According to the breeding method and device, the transparent glass container is used as the breeding tool for orius similis, and provides an air permeable and light permeable breeding environment for orius similis, and experimental observation is facilitated; besides, honey water on the nutrition supplementation bar provides nutrition for orius similis, so that the survival rate of orius similis is increased; the kidney bean seeds are laid at the bottom of the glass container, and can be used as a sheltered site for orius similis, the intraspecific mutual slaughter of orius similis is avoided; besides, orius similis can prey fond frankliniella occidentalis on the kidney bean seeds, and mass propagation is facilitated.

The invention belongs to the field of agricultural biotechnology, and specifically relates to a one-step dual-specificity SS-COI primer combination of the frankliniella occidentalis race and applications thereof. The one-step dual-specificity SS-COI primer combination of a frankliniella occidentalis race of the invention comprises a universal upstream primer TFZ6 of the greenhouse race and the lupine race, a specific downstream primer GRZ32 of the greenhouse race and a specific downstream primer LRZ12 of the lupine race. The sizes of one-step dual-specificity PCR primer combination amplification products are respectively 362bp and 541bp. The primer combination has good detection effect on single-grain eggs and newly hatched larvae, improves the accuracy of detection of the frankliniella occidentalis greenhouse and the frankliniella occidentalis lupine race, saves detection time, and is suitable for promotion in the form of a kit in imported plant quarantine, domestic plant quarantine and field control.

The invention belongs to the field of pesticides and aims at providing a low-toxicity pesticide composition with a better control effect on western flower thrips. Missible oil, suspension, micro-emulsion and the like can be prepared by combining spinosad and emamectin benzoate according to the proportion of 1: 1, 1: 3 and 1: 5 and using an appropriate solvent, an emulsifier, a dispersant, a wetting agent, a stabilizer, an anti-freezing agent, a penetrant and the like, as well as other known substances which are beneficial to stability in storage and use and play efficacy of active ingredients, and the pesticide composition has special efficacy for controlling the western flower thrips.

The invention relates to a bionic trapping device for living frankliniella occidentalis. The device comprises a trapping cover (1), a trapping base (2) connected with the trapping cover (1) in a cooperative mode, a peripheral gauze screen (3) fixedly arranged on an opening of the trapping cover (1) and a central gauze screen (4) arranged in the center of the peripheral gauze screen (3); the exterior of the peripheral gauze screen (3) is provided with brackets (5) supporting the screens; the corresponding end of the opening of the trapping cover (1) is fixedly provided with a sleeve ring (6) connected with the trapping base (2) in a cooperative mode; the upper portion of the trapping base (2) is provided with a filament-imitated structure (7) with inverted hooks. The bionic trapping device is simple in structure, convenient to use, low in cost and high in flexibility, the labor efficiency can be significantly improved, the labor intensity is reduced, and the quantity of living frankliniella occidentalis trapped in the wild field is increased. A trapping method for living frankliniella occidentalis ensures the efficiency of agricultural production to the maximum degree with the purposes of achieving minimal resource consumption, most environment-friendly and fastest trapping and highest trapping number.

The invention relates to a device and a method for testing the stomach toxicity and contact toxicity of frankliniella occidentalis. The device comprises a cover (1), a base (2) and a cover gauze element (3) fixedly arranged in the middle of the cover (1); the inner edge of the side wall of the cover (1) or the outer edge of the side wall of the base (2) is provided with a flexible material; the middle of the base (2) is also provided with a base gauze element (8); filter paper (5) is laid on the base gauze element (8). The device has the advantages of simple structure, convenience and quickness in use, low cost and high flexibility, labor efficiency can be obviously improved, labor intensity is lowered, and toxicity testing accuracy is improved. The method disclosed by the invention has a single or combined simultaneous effect on the stomach toxicity and the contact toxicity, and has the characteristics of simpleness in operation, easiness in processing, relatively short period, high accuracy and high detection efficiency.

The invention relates to the field of molecular biology, in particular to a SCAR primer, a detection method and a kit for Frankliniella occidentalis specificity. The nucleotide sequence of the SCAR primer according to the Frankliniella occidentalis specificity is shown as SEQID No:1 and No:2. According to the unique genome DNA sequence of the Frankliniella occidentalis, the invention designs a pair of specific primers which only have proliferation capability aiming at the Frankliniella occidentalis. The invention is supplement and improvement on a Frankliniella occidentalis RFLP (Restricted Fragment Length Polymorphisms) technology and an mtDNA COI technical detection method.

The invention discloses an application of Neoseiulus neoreticuloides Liang et Hu to control of small-sized juice-absorbing pests. The Neoseiulus neoreticuloides Liang et Hu is capable of controlling thrips and assistantly controlling whiteflies and pest mites, and particularly is capable of controlling Frankliniella occidentalis, Odontothrips loti, Bemisia tabaci(Gennadius), Aceria pallida Keifer,Tetranychus truncatus Ehara and Tetranychus urticae Koch. The Neoseiulus neoreticuloides Liang et Hu is easy in massive expanding propagation, thereby being beneficial to control of thrips in China and other small-sized juice-absorbing pests and colonization of natural enemies-predatory mites.

The invention discloses an improved Grocott silver hexosamine staining method and applications thereof. The staining method comprises the steps of preparing paraffin-embedded sections, staining, carrying out dehydrated hyalinizing, and carrying out section sealing, wherein in the step of preparing paraffin-embedded sections, a sliced paraffin section is adhered to a glass slide subjected to APES treatment. The method overcomes the defect that section shedding easily occurs in the process of staining, especially for frankliniella occidentalis with a small somatotype, the non-section-shedding rate is over 70%. In addition, the internal tissues of polypides in sections obtained according to the staining method disclosed by the invention are red, blastospores in polypides are black, therefore, the polypides are clearly visible, and the staining effect is excellent.

The invention discloses a method for large-scale propagation of the small flower bug. In the present invention, western flower thrips and broad bean aphid are combined to feed southern flower bugs, which fully utilizes the advantage of fast growth rate of the broad bean aphid population, and at the same time utilizes western flower thrips to improve the survival rate of newly hatched nymphs and adult reproduction of southern flower bugs The characteristics of the force, that is, feeding the newly hatched nymphs of the southern flower bug to the western flower thrips to improve the survival rate, combined with the 2-5th instar nymphs to feed the broad bean aphid to reduce the feeding cost, and then achieve the purpose of low-cost and high-efficiency breeding of the southern flower bug. Created a set of breeding technology process suitable for large-scale breeding of small flower bugs, which can quickly and efficiently multiply the southern flower bugs in large quantities, and can be used for field control of thrips, spider mites, aphids and other pests.