A kind of indoor breeding method of western flower thrips

Abstract

The invention discloses a method for feeding frankliniella occidentalis indoors. The method comprises the following steps: inserting dianthus carryophyllus plants into a container filled with water; inoculating frankliniella occidentalis adults onto the plants; putting into a breeding cage; after oviposition, taking stems of the dianthus carryophyllus plants as explants; washing with running water; disinfecting with 75% ethyl alcohol and 0.1% mercuric chloride solution; washing with sterile water; then inoculating the explants onto an inducing medium; inducing multiple shoots in a tissue culture chamber under the culture conditions that the temperature is 25+ / -2 DEG C and the photoperiod is 16L:8D, wherein emerged adults are clean tested insects; transferring the tested insects onto dianthus carryophyllus tissue culture bottle seedlings; paving a layer of sterilized sand on the surface of culture medium in a tissue culture bottle; removing the adults 12 hours after the oviposition; putting back the bottle seedlings with eggs into the tissue culture chamber; and culturing until adults are emerged. The method has the advantages that plant tissue culture seedlings are adopted for feeding frankliniella occidentalis larvae and adults, the feed does not need to be replaced frequently, feeding steps are simplified, the feed is sufficient, the feeding efficiency is improved, and the survival rate is high.

Description

technical field

[0001] The invention belongs to the technical field of plant protection and insect breeding, and in particular relates to a method for raising western flower thrips indoors by using tissue culture bottle seedlings. Background technique

[0002] Western flower thrips [Frankliniella occidentalis (Pergande)] belongs to Thysanoptera (Thysanoptera) Thripidae (Thripidae) Flower Thrips (Frankliniella), is a dangerous worldwide quarantine pests, can damage more than 500 kinds of plants, The damage is especially serious on vegetables and flowers. In mainland China, it was first discovered in Beijing greenhouse peppers in 2003, and it has been reported in many provinces. Western flower thrips has three main ways of harming plants. One is to lay eggs inside the plant tissue and form wounds on the plant surface, which is easy to cause bacterial infection; the other is to feed directly and consume plant nutrients, causing spots, leaf curls, Symptoms such as wilting and ...

Examples

Embodiment 1

[0017] The concrete steps of the inventive method are as follows:

[0018] (1) Obtain a clean source of test insects

[0019] Using carnation plants free from diseases and insect pests as the oviposition substrate, inserting the lower part of the carnation plants into a container filled with water to keep water, then inoculating adults of Thrips occidentalis on the plants of Carnation, and then inoculating thrips occidentalis Put the carnation plant of adult insects together with the container into the insect culture box, lay eggs for 2 days, take the stem section of the carnation plant and strip off the leaves, take the stem section as an explant, wash it with tap water for 10 minutes, and then put it on the ultra-clean workbench, Disinfect the explants with alcohol with a volume fraction of 75% for 5 seconds, then sterilize with a mercuric chloride solution with a mass fraction of 0.1% for 20 minutes, rinse with sterile water for 5 to 6 times, and rinse the explants with ste...

technical field

[0001] The invention belongs to the technical field of plant protection and insect breeding, and in particular relates to a method for raising western flower thrips indoors by using tissue culture bottle seedlings. Background technique

[0002] Western flower thrips [Frankliniella occidentalis (Pergande)] belongs to Thysanoptera (Thysanoptera) Thripidae (Thripidae) Flower Thrips (Frankliniella), is a dangerous worldwide quarantine pests, can damage more than 500 kinds of plants, The damage is especially serious on vegetables and flowers. In mainland China, it was first discovered in Beijing greenhouse peppers in 2003, and it has been reported in many provinces. Western flower thrips has three main ways of harming plants. One is to lay eggs inside the plant tissue and form wounds on the plant surface, which is easy to cause bacterial infection; the other is to feed directly and consume plant nutrients, causing spots, leaf curls, Symptoms such as wilting and ...

Examples

Embodiment 1

[0017] The concrete steps of the inventive method are as follows:

[0018] (1) Obtain a clean source of test insects

[0019] Using carnation plants free from diseases and insect pests as the oviposition substrate, inserting the lower part of the carnation plants into a container filled with water to keep water, then inoculating adults of Thrips occidentalis on the plants of Carnation, and then inoculating thrips occidentalis Put the carnation plant of adult insects together with the container into the insect culture box, lay eggs for 2 days, take the stem section of the carnation plant and strip off the leaves, take the stem section as an explant, wash it with tap water for 10 minutes, and then put it on the ultra-clean workbench, Disinfect the explants with alcohol with a volume fraction of 75% for 5 seconds, then sterilize with a mercuric chloride solution with a mass fraction of 0.1% for 20 minutes, rinse with sterile water for 5 to 6 times, and rinse the explants with ste...

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