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Culture method of sclerotium of beauveria bassiana and application thereof

A technology of Beauveria bassiana and its culture method, which is applied to the cultivation of entomogenous fungus sclerotia and the application field of pest control, can solve the problems of no pathogenic fungus-Beauveria bassiana MS culture and application reports, and achieve low cost , simple process and broad application prospects

Inactive Publication Date: 2011-10-05
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no report on the culture and application of Beauveria bassiana MS, an important pathogenic fungus in the soil-dwelling stage of the control of Thrips occidentalis

Method used

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  • Culture method of sclerotium of beauveria bassiana and application thereof
  • Culture method of sclerotium of beauveria bassiana and application thereof
  • Culture method of sclerotium of beauveria bassiana and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The cultivation of embodiment 1 Beauveria bassiana sclerotia:

[0023] 1. Training method:

[0024] 1.1 Primary plate culture

[0025]Beauveria bassiana strains were grown on PDA medium at room temperature (~22°C) for 3 weeks. Cut sporulated agar plates to 1mm 2 Agar blocks were submerged in 10% glycerol and stored at -80°C.

[0026] 1.2 Preparation of spore suspension

[0027] For liquid culture, inoculate frozen stored agar blocks on PDA plates and culture at room temperature (~22°C) for 2 to 3 weeks. Add sterile water containing 0.1% polyethylene glycol oleic acid-based compound (Tween-80, Sigma), and aseptically disperse, mix and dilute to make a final concentration of 1.0×10 9 L -1 The spore suspension was used for later use.

[0028] 1.3 Sclerotia in liquid culture

[0029] In a 250mL beaker, put 100mL of culture solution into the spore suspension to make the final concentration of spores reach 5×10 6 L -1 , placed on a constant temperature shaker (tempe...

Embodiment 2

[0045] Embodiment 2 bioassay test:

[0046] 1. Detection of bioassay efficiency:

[0047] Breeding of Thrips biflora: Put 3 to 4 bean pods in a 0.5L cylindrical glass container, transfer adult thrips into the container to lay eggs, place the container in a growth box, and set the culture conditions at a temperature of 26°C. The relative humidity is 60%-70%, the photoperiod is L:D=13:11, and the adults are removed after 12 hours of oviposition to ensure that the larval ages are consistent. After 8 days, the 2nd instar and 2d larvae (L2) were collected for use.

[0048] Mix vermiculite, clay loam and nutrient soil in a ratio of 2:1:1. Add MS-DE particles evenly into the mixed soil, the mixing ratio is 100μg / 100g soil. Put the above mixture into a plastic flower pot, and spray ion-free water evenly until water seeps out from the bottom of the pot. Place the pots in plastic trays and add water frequently to keep the trays constantly filled with water that can be siphoned into ...

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Abstract

Aiming at the defects of conidiospores as propagula of an insecticide of insect pathogenic fungi, the invention provides a technology of liquid culture of entomogenous fungus sclerotia, and the entomogenous fungus can be beauveria bassiana. The liquid culture method of entomogenous fungus sclerotia provided by the invention is required to control the liquid culture components and the culture conditions within a certain range. The cultured sclerotia can be stored for a long time after vacuum drying, and can still germinate mycelia and pathogenic conidiospores under suitable conditions. The micro-sclerotia prepared by drying have maintained activity, can germinate mycelia and spores after rehydration, and increase the potential of beauveria bassiana as a biocontrol factor for western flowerthrips in a soil-dwelling phase. The technology has low cost, long storage period of sclerotia, and is quite suitable for popularization.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for cultivating entomogenic fungus sclerotia and its application in pest control. Background technique [0002] Western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) is an important pest on a variety of commercial crops worldwide. Western flower thrips cause serious damage to crops by feeding and spreading plant viruses through files. The life cycle of Thrips occidentalis includes above-ground feeding stages (adults, 1st and 2nd instar nymphs) and soil-dwelling stages (end of 2nd instar nymphs, prepupa and pupae). Most of the mature 2nd instar nymphs of T. occidentalis showed positive geotaxis and negative phototaxis, and moved from plants to soil or potting medium to pupate. Depending on the species of host plants, up to 98% of thrips pupate underground and distribute in the soil layer at a depth of 1.5-2.0 cm until they...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 王海鸿雷仲仁问锦曾
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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