Beaueria bassaria (Balsamo) Vuillemin bacterial strain GZGY-1-3 and application thereof

Abstract

The invention relates to a Beaueria bassaria (Balsamo) Vuillemin bacterial strain GZGY-1-3, a culture method of the bacterial strain, spore powder prepared from the bacterial strain and application of the spore powder in preparing a biocontrol agent, wherein the biocontrol agent can be used for preventing and controlling bemisia tabaci, frankliniella occidentalis, green onion aphidand / or green peach aphid. The biocontrol agent can be used for preventing and controlling insects under a high temperature environment, and is good in prevention and control effect with field control efficiency over 75%.

Description

technical field

[0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a Beauveria bassiana strain GZGY-1-3 and its application in preventing and controlling Bemisia tabaci, Western flower thrips, green peach aphid and green onion aphid. Background technique

[0002] Bemisa tabaci (Gennadius), western flower thrips Frankliniella occidentalis (Pergand) and green peach aphid Myzus persicae (Sulzer) are all important agricultural pests worldwide, and their hosts include various economic crops such as vegetables, flowers and tobacco. The green onion aphid Neotox optera formosana (Takahashi) is one of the pests that cause more damage to shallots, garlic and leeks in my country in recent years. The common feature of these pests is that they feed on plant sap with piercing or file-sucking mouthparts and cause serious direct damage to crops. What is more serious is that they, as the vector of plant virus diseases, cause great damage to ...

Examples

Embodiment 1

[0029] Example 1 Screening for strains with high virulence

[0030] 1.1 Insect populations

[0031]Bemisia tabaci was collected from tobacco in the greenhouse of the Langfang Research Base of the Chinese Academy of Agricultural Sciences in 2013, and was raised with cabbage seedlings for 2-3 generations in this laboratory. Cabbage seedlings were placed in insect cages (40×40×30 cm), the feeding conditions were 26±2°C, and the photoperiod was 12:12 (L:D). In order to make enough and uniform Bemisia tabaci nymphs on the cabbage leaves for the toxicity test of Beauveria bassiana, the cabbage seedlings with a height of about 25 cm and 5-6 young leaves were selected during the test and placed in pitchers. After 48 hours, take out and drive away all adults, so that there are about 100-150 eggs on each leaf. The taken out seedlings were placed in a light incubator under the conditions of 26±1°C, 12:12 (L:D), and the leaves were cut off when the eggs of Bemisia tabaci developed into ...

Embodiment 2

[0049] Example 2 Screening the strains with high spore production

[0050] 2.1 Strain cultivation

[0051] The spores of 12 strains of highly virulent strains screened in the above-mentioned embodiment 1 were formulated with 0.05% Tween-80 solution into 1 × 10 6 Conidia / ml of suspension. Take 0.1ml of the suspension on the 90mm SDAY medium and spread it evenly with a sterile triangular glass rod. Each strain was replicated 4 times. The culture medium was cultured in the dark at 26±1°C for 15 days, and 5 points were randomly taken from the culture medium with a sterile puncher with a diameter of 4 mm and put into 5 ml of 0.05% Tween-80 solution. The suspension was sonicated in an ultrasonic cleaner for 15 minutes to destroy the structure of the spore block, and then vibrated with a vortex oscillator for 10 minutes to obtain a uniformly dispersed spore suspension. After the suspension was diluted 10 times, the number of spores was measured with a hemocytometer, each count wa...

Embodiment 3

[0055] Example 3 Screening for heat-tolerant bacterial strains

[0056] 3.1 Screening of heat-tolerant strains

[0057] This example refers to the method of Everton K.K et al (2008). The 6 strains with high toxicity to whitefly in the above-mentioned Example 2 were selected, inoculated on SDA medium, and cultured at 26±1° C. for 15 days in the dark. The harvested spores were prepared into a suspension with 0.05% Tween-80 solution, the suspension was vigorously shaken and filtered, and then diluted to 10 5 spores / mi. Take 2ml of the suspension in a 5ml centrifuge tube and immediately place it in a water bath at 45±0.1°C. After heat shock for 1h or 2h, take 20μl and drop it in the center of 4ml SDAY medium, the SDAY medium is SDA medium with 1% yeast extract, the diameter of the petri dish is 35mm, the SDAY medium has been high-temperature sterilized in advance and contains the concentration Benomyl is 0.002% (w / v), active ingredient 25%. Low concentrations of benomyl will ...

technical field

[0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a Beauveria bassiana strain GZGY-1-3 and its application in preventing and controlling Bemisia tabaci, Western flower thrips, green peach aphid and green onion aphid. Background technique

[0002] Bemisa tabaci (Gennadius), western flower thrips Frankliniella occidentalis (Pergand) and green peach aphid Myzus persicae (Sulzer) are all important agricultural pests worldwide, and their hosts include various economic crops such as vegetables, flowers and tobacco. The green onion aphid Neotox optera formosana (Takahashi) is one of the pests that cause more damage to shallots, garlic and leeks in my country in recent years. The common feature of these pests is that they feed on plant sap with piercing or file-sucking mouthparts and cause serious direct damage to crops. What is more serious is that they, as the vector of plant virus diseases, cause great damage to ...

Examples

Embodiment 1

[0029] Example 1 Screening for strains with high virulence

[0030] 1.1 Insect populations

[0031]Bemisia tabaci was collected from tobacco in the greenhouse of the Langfang Research Base of the Chinese Academy of Agricultural Sciences in 2013, and was raised with cabbage seedlings for 2-3 generations in this laboratory. Cabbage seedlings were placed in insect cages (40×40×30 cm), the feeding conditions were 26±2°C, and the photoperiod was 12:12 (L:D). In order to make enough and uniform Bemisia tabaci nymphs on the cabbage leaves for the toxicity test of Beauveria bassiana, the cabbage seedlings with a height of about 25 cm and 5-6 young leaves were selected during the test and placed in pitchers. After 48 hours, take out and drive away all adults, so that there are about 100-150 eggs on each leaf. The taken out seedlings were placed in a light incubator under the conditions of 26±1°C, 12:12 (L:D), and the leaves were cut off when the eggs of Bemisia tabaci developed into ...

Embodiment 2

[0049] Example 2 Screening the strains with high spore production

[0050] 2.1 Strain cultivation

[0051] The spores of 12 strains of highly virulent strains screened in the above-mentioned embodiment 1 were formulated with 0.05% Tween-80 solution into 1 × 10 6 Conidia / ml of suspension. Take 0.1ml of the suspension on the 90mm SDAY medium and spread it evenly with a sterile triangular glass rod. Each strain was replicated 4 times. The culture medium was cultured in the dark at 26±1°C for 15 days, and 5 points were randomly taken from the culture medium with a sterile puncher with a diameter of 4 mm and put into 5 ml of 0.05% Tween-80 solution. The suspension was sonicated in an ultrasonic cleaner for 15 minutes to destroy the structure of the spore block, and then vibrated with a vortex oscillator for 10 minutes to obtain a uniformly dispersed spore suspension. After the suspension was diluted 10 times, the number of spores was measured with a hemocytometer, each count wa...

Embodiment 3

[0055] Example 3 Screening for heat-tolerant bacterial strains

[0056] 3.1 Screening of heat-tolerant strains

[0057] This example refers to the method of Everton K.K et al (2008). The 6 strains with high toxicity to whitefly in the above-mentioned Example 2 were selected, inoculated on SDA medium, and cultured at 26±1° C. for 15 days in the dark. The harvested spores were prepared into a suspension with 0.05% Tween-80 solution, the suspension was vigorously shaken and filtered, and then diluted to 10 5 spores / mi. Take 2ml of the suspension in a 5ml centrifuge tube and immediately place it in a water bath at 45±0.1°C. After heat shock for 1h or 2h, take 20μl and drop it in the center of 4ml SDAY medium, the SDAY medium is SDA medium with 1% yeast extract, the diameter of the petri dish is 35mm, the SDAY medium has been high-temperature sterilized in advance and contains the concentration Benomyl is 0.002% (w / v), active ingredient 25%. Low concentrations of benomyl will ...

Images