Method for quick mycorhiza formation of azalea aseptic seedlings

A sterile seedling and mycorrhizal technology, applied in the field of botany, can solve the problem of strains growing too fast, affecting the process of seedling mycorrhiza and later growth, etc. high effect

Active Publication Date: 2012-10-03
SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in later experiments, it was found that the single-layer inoculation method of Rhododendron yunnanensis often caused the strain to grow too fast, which affected the mycorrhizal process and later growth of the seedlings.

Method used

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  • Method for quick mycorhiza formation of azalea aseptic seedlings
  • Method for quick mycorhiza formation of azalea aseptic seedlings
  • Method for quick mycorhiza formation of azalea aseptic seedlings

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Experimental program
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Effect test

Embodiment 1

[0020] The isolation and identification of embodiment 1 rhododendron mycorrhizal fungus:

[0021] 1. Separation of root materials: Take the living roots of the rhododendron species ‘Purple Crane’ in Shanghai Binjiang Forest Park, put them in an ice bucket together with soil moisture, and store them in a 4°C refrigerator for later use.

[0022] 2. Separation and identification medium:

[0023] (1) Separation medium: Use Martin-Bengal red medium (referred to as MA) to adjust and improve its pH. The formula is: 10 grams of glucose, 5 grams of tryptone, 1 gram of dipotassium hydrogen phosphate, 0.5 grams of magnesium sulfate, 0.033 grams of Bengal red, 20 grams of agar, distilled water to 1000 ml, adjust the pH value to 5.0, and then 121 ℃ high pressure Sterilize for 20 minutes, add 0.03 g of streptomycin when cooled to below 60°C, mix well, and pour it into a plate for later use.

[0024] (2) Strain identification medium: use malt extract medium (MEA), the formula is: 20g of ma...

Embodiment 2

[0042] The screening of embodiment 2 suitable cultivation substrates

[0043] Choose peat, vermiculite and yellow sand three common cultivation substrates and mix them in different proportions as the ready-to-use cultivation substrate. The specific formula is peat: vermiculite 2:1, peat: vermiculite: yellow sand 2:1:1, peat: yellow sand 2:1, peat: yellow sand 3:1. After the substrate is mixed in proportion, it is sterilized at high temperature, and then poured into the sterilized MMN liquid medium, 300ml / 1000g of cultivation substrate, mixed evenly, and then packed into cultivation bottles. Transplant aseptic sowing seedlings with a height of 2-4cm, and inoculate one bacterial block (diameter 2-4mm). Any one of the fungal strains, 30 seedlings were cultivated in each substrate, and the growth of the seedlings in different substrates was counted after 12 weeks (Table 1). The growth potential of the seedlings in the cultivation substrate containing vermiculite was poor, and the...

Embodiment 4

[0046] The screening of glucose concentration in embodiment 4MMN medium

[0047] Nutrients and contents in MMN liquid medium were unchanged except for glucose.

[0048] The formula of MMN liquid medium is: calcium chloride (Cacl2 2H2O) 0.05g, magnesium sulfate (MgSO4 7H2O) 0.15g, sodium chloride (NaCl) 0.025g, ferric chloride (FeCl3) 0.012g, diphosphate Potassium hydrogen (KH2PO4) 0.5g, vitamin B10.1mg, diammonium hydrogen phosphate ((N H4)2HPO4) 0.25g, citric acid 0.2g, malt extract 2g, distilled water to 1000ml.

[0049] The amount of glucose added in 1L medium was adjusted to 0.5g (0.05%), 1g (0.1%), 3g (0.3%), 5g (0.5%), 10g (1%) and 15g (1.5%) respectively. Using peat: yellow sand 3:1 as the substrate, pour 300ml / L of MMN medium with different glucose content, mix the substrate and nutrient solution evenly, then pack into tissue culture bottles, and then sterilize at high temperature. Transplant 4 seedlings into each bottle, insert 1 bacterial block between every two se...

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Abstract

The invention belongs to the field of botany, and provides a method for the quick mycorhiza formation of azalea aseptic seedlings. By the method, the problems of high death rate, low growth speed, yellowing of laminae and the like of azalea seedlings in the prior art are solved. The method comprises the following steps of: preparing a cultivation medium and a nutrient solution; and transplanting rooted seedlings which are sown or subjected to tissue culture into the cultivation medium which is subjected to aseptic treatment, inoculating at least one bacterial block, and placing the seedlings in a culture chamber for culturing, wherein the cultivation medium consists of grass carbon and yellow sand in a weight ratio of (2-3):1; in the nutrient solution, the concentration of glucose is 0.1 to 0.3 percent; and the bacterial block is selected from any one of CGMCC NO: 6010 or 6031, and the inoculating position of the bacterial block is 0.5 to 1.0 centimeter away from a root system. By themethod, mycorrhiza of the azalea seedlings can be formed quickly and is high in infection rate, and the growth of the seedlings is quick, so the method can be used for scientific study and also can be used for the production of annual seedlings of azalea.

Description

Technical field: [0001] The invention belongs to the field of botany, and in particular relates to a rhododendron cultivation technology, in particular to a method for rapid mycorrhization of rhododendron aseptic seedlings. Background technique: [0002] Rhododendron is a world-renowned potted flower and landscaping plant. In my country, it is a traditional famous flower with the reputation of "beauty among flowers". It is deeply loved by people and has a large demand for seedlings. The breakthrough of sowing propagation and tissue culture technology is the key link of rhododendron variety breeding and seedling production. At present, many Rhododendron species can be propagated by seeding and tissue culture, but the slow growth of seedlings, low survival rate of transplanting, and yellowing of leaves are the key factors that limit the efficiency of variety selection and seedling production. [0003] Rhododendron is a typical mycorrhizal plant. The formation of mycorrhizae c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00C12N1/14C12R1/645
Inventor 张春英黄芳尹丽娟陈真杨兵
Owner SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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