The invention discloses an efficient one-step regeneration method taking a
root system of
broussonetia papyrifera as an explant. The method comprises the following steps: S1, adventitious
bud induction: selecting a
root system of a
broussonetia papyrifera aseptic
seedling as the explant,
cutting out a wound, inoculating the explant to an adventitious
bud induction medium, and conducting inducing to form adventitious buds; and S2, rooting culture:
cutting the adventitious buds in the step S1, inoculating the adventitious buds to a rooting culture medium for rooting induction, and culturing the adventitious buds until the adventitious buds root to form regenerated seedlings. According to the method, the
broussonetia papyrifera in-vitro regeneration
plant is successfully obtained through the steps of adventitious
bud induction, adventitious bud rooting,
domestication and
transplanting and the like by taking the
root system of the broussonetia papyrifera as the explant, and compared with a culture method taking other broussonetia papyrifera tissues as explants, the method is simpler and more convenient to operate, wider in material taking range and more efficient in propagation.