Efficient one-step regeneration method taking root system of broussonetia papyrifera as explant

A technology for explants and paper mulberry roots, applied in the field of plant tissue culture, can solve the problems of low proliferation coefficient and unstable induction rate, and achieve the effects of increasing the proliferation coefficient, shortening the regeneration time, and taking a wide range of materials.
CN113207690AActive Publication Date: 2021-08-06SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2021-08-06

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Abstract

The invention discloses an efficient one-step regeneration method taking a root system of broussonetia papyrifera as an explant. The method comprises the following steps: S1, adventitious bud induction: selecting a root system of a broussonetia papyrifera aseptic seedling as the explant, cutting out a wound, inoculating the explant to an adventitious bud induction medium, and conducting inducing to form adventitious buds; and S2, rooting culture: cutting the adventitious buds in the step S1, inoculating the adventitious buds to a rooting culture medium for rooting induction, and culturing the adventitious buds until the adventitious buds root to form regenerated seedlings. According to the method, the broussonetia papyrifera in-vitro regeneration plant is successfully obtained through the steps of adventitious bud induction, adventitious bud rooting, domestication and transplanting and the like by taking the root system of the broussonetia papyrifera as the explant, and compared with a culture method taking other broussonetia papyrifera tissues as explants, the method is simpler and more convenient to operate, wider in material taking range and more efficient in propagation.
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Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-efficiency one-step regeneration method using the root system of a mulberry tree as an explant. Background technique

[0002] Broussonetia Papyrifera (L.) Vent., also known as hair peach, mulberry peach, paper mulberry, valley tree, etc., is a perennial deciduous tree belonging to the family Moraceae. The adult tree is 10-20m high; the bark is dark gray; the whole plant contains latex; the branchlets are densely pilose; the leaves are broadly ovate to oblong-ovate and spirally arranged, the apex is acuminate, the base is heart-shaped, and the edge is coarsely serrated. The leaves of small trees often have obvious 3-5 lobes, and the back is densely covered with fluff; the petiole is 2.5-8cm long and densely covered with strigose; flowers are dioecious; the male inflorescence is a catkin, and the female inflorescence is spherical-headed; the fleshy c...

Claims

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