Efficient one-step regeneration method taking root system of broussonetia papyrifera as explant

Abstract

The invention discloses an efficient one-step regeneration method taking a root system of broussonetia papyrifera as an explant. The method comprises the following steps: S1, adventitious bud induction: selecting a root system of a broussonetia papyrifera aseptic seedling as the explant, cutting out a wound, inoculating the explant to an adventitious bud induction medium, and conducting inducing to form adventitious buds; and S2, rooting culture: cutting the adventitious buds in the step S1, inoculating the adventitious buds to a rooting culture medium for rooting induction, and culturing the adventitious buds until the adventitious buds root to form regenerated seedlings. According to the method, the broussonetia papyrifera in-vitro regeneration plant is successfully obtained through the steps of adventitious bud induction, adventitious bud rooting, domestication and transplanting and the like by taking the root system of the broussonetia papyrifera as the explant, and compared with a culture method taking other broussonetia papyrifera tissues as explants, the method is simpler and more convenient to operate, wider in material taking range and more efficient in propagation.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-efficiency one-step regeneration method using the root system of a mulberry tree as an explant. Background technique [0002] Broussonetia Papyrifera (L.) Vent., also known as hair peach, mulberry peach, paper mulberry, valley tree, etc., is a perennial deciduous tree belonging to the family Moraceae. The adult tree is 10-20m high; the bark is dark gray; the whole plant contains latex; the branchlets are densely pilose; the leaves are broadly ovate to oblong-ovate and spirally arranged, the apex is acuminate, the base is heart-shaped, and the edge is coarsely serrated. The leaves of small trees often have obvious 3-5 lobes, and the back is densely covered with fluff; the petiole is 2.5-8cm long and densely covered with strigose; flowers are dioecious; the male inflorescence is a catkin, and the female inflorescence is spherical-headed; the fleshy c...

AI Technical Summary

Problems solved by technology

There is only one case report (patent publication number is CN 108812260 B) of constructing the regeneration system with the root system of the mulberry root system as the explant. In open seedling cultivation, root segments are used in the whole process of hardening and transplanting instead of regenerated seedlings, which is more like root sprouting and reproduction, rather than the concept of tissue culture in the general sense, and the induction rate of root sprouting is unstable, Problems such as low multiplication coefficient

In order to solve the problems existing in the current propagation methods of mulberry tree using seedlings, cuttings, root propagation, etc., and to solve the problems existing in the propagation technology of using hybrid tree stems, young stems, leaves and other parts as explants for tissue culture , the present invention provides a high-efficiency one-step regeneration method using the root system of mulberry tree as explant

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