Tissue culture and rapid propagation method of antifebrile dichroa

A technology of tissue culture and Changshan, which is applied in the field of tissue culture and rapid propagation of Changshan, can solve the problems of unstable genetic traits, loss of good traits of parents, and separation of traits, and achieve the effect of promoting industrialization development

Inactive Publication Date: 2019-03-22
韦宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Changshan seedlings are mainly propagated by seeds and cuttings. Although a large number of seedlings can be obtained in a short period of time by seed sowing, because Changshan is a monoecious cross-pollinated plant, the offspring are prone to segregation of traits, and the genetic traits are unstable and easy to be lost. Good traits of parents
However, the cutting method requires a large number of branches, and it takes 2 months from cutting to transplanting in the field, which takes a long time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Induction of adventitious buds: select the explants of Changshan cane with joints in the same year, sterilize them with 75% alcohol for 18 seconds, rinse them with sterile water for 5 times, and put them in 0.1% mercuric chloride solution for 20 minutes. , and then rinsed with sterile water 10 times, dried by sterile filter paper, cut into 6cm canes with joints, and inoculated on the induction medium, cultured in the dark at 25°C for 22 days to induce Adventitious buds are formed, the pollution rate is as low as 6%, and the adventitious bud induction rate can reach 93%. The induction medium is: MS+0.3mg / LTDZ+1.5mg / L 6-BA+1.5mg / LNAA+25g / L sucrose+5.5g / L agar, pH is 5.2;

[0013] (2) Subculture: cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, place them in the light for 15 hours a day after inoculation, the light intensity is 2200 lx, and the culture temperature is 25 Cultivate under the con...

Embodiment 2

[0017] (1) Induction of adventitious buds: select the explants of Changshan cane with joints in the same year, sterilize them with 75% alcohol for 20 seconds, rinse them with sterile water for 5 times, and put them in 0.5% mercuric chloride solution for 20 minutes. , and then rinsed with sterile water 8 times, dried by sterile filter paper, cut into 6 cm canes with joints, and inoculated on the induction medium, cultured at 26°C for 18 days in the dark to induce Adventitious buds are formed, the pollution rate is as low as 8%, and the adventitious bud induction rate can reach 96%. The induction medium is: MS+0.6mg / LTDZ+1.8mg / L 6-BA+0.5mg / LNAA+25g / L sucrose+5.0g / L agar, with a pH of 5.4.

[0018] (2) Subculture: Cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, and place them in the light for 18 hours a day after inoculation, the light intensity is 2500 lx, and the culture temperature is 26 Cultivate un...

Embodiment 3

[0022] (1) Induction of adventitious buds: select the explants of Changshan cane with joints in the same year, sterilize them with 75% alcohol for 22 seconds, rinse them with sterile water for 5 times, and put them in 0.2% mercuric chloride solution for 33 minutes. , and then washed 7 times with sterile water, dried by sterile filter paper, cut into 6cm canes with joints, and inoculated on the induction medium, cultured at 28°C in the dark for 23 days to induce Adventitious buds are formed, the pollution rate is as low as 3%, and the adventitious bud induction rate can reach 88%. The induction medium is: MS+0.4mg / LTDZ+1.6mg / L 6-BA+0.5mg / LNAA+28g / L sucrose+4.8g / L agar, with a pH of 5.4.

[0023] (2) Subculture: Cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, and place them under light for 16 hours a day after inoculation, with a light intensity of 2200 lx and a culture temperature of 28 Cultivate unde...

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Abstract

The invention discloses a tissue culture and rapid propagation method of antifebrile dichroa. The antifebrile dichroa is a plant of saxifragaceae, and is produced in Hunan, Guangxi, Guizhou, Sichuan and Jiangxi. At present, seedlings of the antifebrile dichroa are mainly propagated in ways of seeds and cuttage, so that problems such as long cycle, high cost and low efficiency are caused. Therefore, stem segments with nodes are taken as explants, and in vitro replanted plants of the antifebrile dichroa are successfully obtained by the processes of adventitious bud induction, proliferation, rooting, acclimatization and transplanting, and the like; a tissue culture and rapid propagation technology system of the antifebrile dichroa is established. The tissue culture and rapid propagation method has a great significance for the rapid propagation and large-scale promotion of good varieties and the promotion of industrialization development.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a rapid propagation method for tissue culture in Changshan. Background technique [0002] Changshan is a plant of the saxifrage family. Its dried roots are produced in Hunan, Guangxi, Guizhou, Sichuan, and Jiangxi. Excavated in autumn, removed fibrous roots and aboveground parts, washed and dried in the sun. Odorless, bitter taste. Cure mainly cut malaria, eliminate phlegm. At present, Changshan seedlings are mainly propagated by seeds and cuttings. Although a large number of seedlings can be obtained in a short period of time by seed sowing, because Changshan is a monoecious cross-pollinated plant, the offspring are prone to segregation of traits, and the genetic traits are unstable and easy to be lost. Excellent traits of the parents. The cutting method requires a large number of branches, and it takes 2 months from cutting to transplanting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 韦宇
Owner 韦宇
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