Tissue culture and rapid propagation method of antifebrile dichroa
A technology of tissue culture and Changshan, which is applied in the field of tissue culture and rapid propagation of Changshan, can solve the problems of unstable genetic traits, loss of good traits of parents, and separation of traits, and achieve the effect of promoting industrialization development
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Embodiment 1
[0012] (1) Induction of adventitious buds: select the explants of Changshan cane with joints in the same year, sterilize them with 75% alcohol for 18 seconds, rinse them with sterile water for 5 times, and put them in 0.1% mercuric chloride solution for 20 minutes. , and then rinsed with sterile water 10 times, dried by sterile filter paper, cut into 6cm canes with joints, and inoculated on the induction medium, cultured in the dark at 25°C for 22 days to induce Adventitious buds are formed, the pollution rate is as low as 6%, and the adventitious bud induction rate can reach 93%. The induction medium is: MS+0.3mg / LTDZ+1.5mg / L 6-BA+1.5mg / LNAA+25g / L sucrose+5.5g / L agar, pH is 5.2;
[0013] (2) Subculture: cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, place them in the light for 15 hours a day after inoculation, the light intensity is 2200 lx, and the culture temperature is 25 Cultivate under the con...
Embodiment 2
[0017] (1) Induction of adventitious buds: select the explants of Changshan cane with joints in the same year, sterilize them with 75% alcohol for 20 seconds, rinse them with sterile water for 5 times, and put them in 0.5% mercuric chloride solution for 20 minutes. , and then rinsed with sterile water 8 times, dried by sterile filter paper, cut into 6 cm canes with joints, and inoculated on the induction medium, cultured at 26°C for 18 days in the dark to induce Adventitious buds are formed, the pollution rate is as low as 8%, and the adventitious bud induction rate can reach 96%. The induction medium is: MS+0.6mg / LTDZ+1.8mg / L 6-BA+0.5mg / LNAA+25g / L sucrose+5.0g / L agar, with a pH of 5.4.
[0018] (2) Subculture: Cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, and place them in the light for 18 hours a day after inoculation, the light intensity is 2500 lx, and the culture temperature is 26 Cultivate un...
Embodiment 3
[0022] (1) Induction of adventitious buds: select the explants of Changshan cane with joints in the same year, sterilize them with 75% alcohol for 22 seconds, rinse them with sterile water for 5 times, and put them in 0.2% mercuric chloride solution for 33 minutes. , and then washed 7 times with sterile water, dried by sterile filter paper, cut into 6cm canes with joints, and inoculated on the induction medium, cultured at 28°C in the dark for 23 days to induce Adventitious buds are formed, the pollution rate is as low as 3%, and the adventitious bud induction rate can reach 88%. The induction medium is: MS+0.4mg / LTDZ+1.6mg / L 6-BA+0.5mg / LNAA+28g / L sucrose+4.8g / L agar, with a pH of 5.4.
[0023] (2) Subculture: Cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, and place them under light for 16 hours a day after inoculation, with a light intensity of 2200 lx and a culture temperature of 28 Cultivate unde...
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