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Tissue culture and rapid propagation method of raisin tree seed

A technology of tissue culture and daffodil, applied in the field of plant tissue culture, can solve the problems of unstable genetic traits, long time consumption, loss of excellent parental traits, etc., and achieve the effect of promoting industrialization development.

Inactive Publication Date: 2019-03-22
韦宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the seedlings of Chiluzi seedlings are mainly propagated by seeds and cuttings. Although a large number of seedlings can be obtained in a short period of time by sowing seeds, the offspring of Chiluluzi are prone to segregation of traits, unstable genetic traits, and easy to lose the excellent traits of the parents.
However, the cutting method requires a large number of branches, and it takes 2 months from cutting to transplanting in the field, which takes a long time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Induction of adventitious buds: select the explants of the jointed rattans of Chilus chinensis, sterilize them with 75% alcohol for 15 seconds, rinse them with sterile water for 6 times, and place them in 0.1% mercuric chloride solution for disinfection. 18 minutes, then rinsed with sterile water 8 times, blotted dry with sterile filter paper, cut into about 7 cm canes with joints, inoculated on the induction medium, and cultured in the dark at 25°C for 21 days. It can induce the formation of adventitious buds, the pollution rate is as low as 8%, and the adventitious bud induction rate can reach 93%. The induction medium is: MS+0.5mg / LTDZ+1.5mg / L 6-BA+0.8mg / LNAA+23g / L sucrose+4.8g / L agar, pH is 5.6;

[0013] (2) Subculture: cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, and place them in the light for 16 hours a day after inoculation, the light intensity is 1800 lx, and the culture tempera...

Embodiment 2

[0017] (1) Induction of adventitious buds: select the explants of Chilus chinensis with joints in the same year, disinfect them with 75% alcohol for 17 seconds, rinse them with sterile water for 6 times, and place them in 0.1% mercuric chloride solution for disinfection After 17 minutes, rinse with sterile water for 8 times, dry the water through sterile filter paper, cut into 6 cm canes with joints, inoculate them on the induction medium, and culture them in the dark at 26°C for 16 days. It can induce the formation of adventitious buds, the pollution rate is as low as 9%, and the adventitious bud induction rate can reach 96%. The induction medium is: MS+0.6mg / LTDZ+1.8mg / L 6-BA+0.5mg / LNAA+22g / L sucrose+4.2g / L agar, with a pH of 5.7.

[0018] (2) Subculture: cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, and place them in the light for 16 hours a day after inoculation, the light intensity is 2200 lx, ...

Embodiment 3

[0022] (1) Induction of adventitious buds: select the explants of the sclerotinia japonica in the same year as the explants, sterilize them with 75% alcohol for 23 seconds, rinse them with sterile water for 7 times, and put them in 0.1% mercuric chloride solution for disinfection After 33 minutes, rinse with sterile water for 9 times, dry the water through sterile filter paper, cut into 7 cm canes with joints, inoculate them on the induction medium, and culture them in the dark at 28°C for 23 days. It can induce the formation of adventitious buds, the pollution rate is as low as 3%, and the adventitious bud induction rate can reach 88%. The induction medium is: MS+0.3mg / LTDZ+1.8mg / L 6-BA+0.6mg / LNAA+28g / L sucrose+4.8g / L agar, with a pH of 5.9.

[0023] (2) Subculture: cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, and place them in the light for 18 hours a day after inoculation, the light intensity is...

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Abstract

The invention discloses a tissue culture and rapid propagation method of raisin tree seed. The seedlings of the raisin tree seed are mainly propagated in ways of seeds and cuttage, so that problems such as long cycle, high cost and low efficiency are caused. Therefore, stem segments with nodes are taken as explants, and in vitro replanted plants of the raisin tree seed are successfully obtained bythe processes of adventitious bud induction, proliferation, rooting, acclimatization and transplanting, and the like; a tissue culture and rapid propagation technology system of the raisin tree seedis established. The tissue culture and rapid propagation method has a great significance for the rapid propagation and large-scale promotion of good varieties of the raisin tree seed and the promotionof industrialization development of the raisin tree seed.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapidly propagating tissue culture of japonica. Background technique [0002] Chiluzi is a Rhamnaceae plant, and its dry and mature seeds are taken. It is mainly produced in Shaanxi, Guangdong, Hubei, Zhejiang, Jiangsu, etc. It is harvested when the fruit is ripe in autumn, the fruit is picked, the shell is crushed, the seeds are sifted out, and dried in the sun. Gas weak, bitter and astringent. It has the functions of sobering up, clearing away heat, quenching thirst and diuresis. For drunkenness, dysphoria, mouth drinking, difficulty urinating. At present, the seedlings of Ducklings are mainly propagated by seeds and cuttings. Although a large number of seedlings can be obtained in a short period of time by sowing seeds, the offspring of Ducklings are prone to segregation of traits, the genetic traits are unstable, and the excell...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 韦宇
Owner 韦宇
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