A high-efficiency one-step regeneration method using mulberry roots as explants

A technology of explants and tree roots, which is applied in the field of plant tissue culture, can solve the problems of low multiplication coefficient and unstable induction rate, etc., and achieve the effects of increasing multiplication coefficient, shortening regeneration time, and convenient material collection

Active Publication Date: 2022-03-11
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is only one case report (patent publication number is CN 108812260 B) of constructing the regeneration system with the root system of the mulberry root system as the explant. In open seedling cultivation, root segments are used in the whole process of hardening and transplanting instead of regenerated seedlings, which is more like root sprouting and reproduction, rather than the concept of tissue culture in the general sense, and the induction rate of root sprouting is unstable, Problems such as low multiplication coefficient
In order to solve the problems existing in the current propagation methods of mulberry tree using seedlings, cuttings, root propagation, etc., and to solve the problems existing in the propagation technology of using hybrid tree stems, young stems, leaves and other parts as explants for tissue culture , the present invention provides a high-efficiency one-step regeneration method using the root system of mulberry tree as explant

Method used

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  • A high-efficiency one-step regeneration method using mulberry roots as explants
  • A high-efficiency one-step regeneration method using mulberry roots as explants
  • A high-efficiency one-step regeneration method using mulberry roots as explants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Inducing adventitious buds: select the root system of the aseptic seedlings of S. mulberry as explants, cut 2 to 3 cuts to increase the wound; inoculate it on the adventitious bud induction medium in a horizontal manner, at a temperature of 25°C and light intensity 2500lx, and the light time is 12h per day for cultivating until adventitious buds are induced. The adventitious bud induction medium is: 1 / 2MS+1.0mg / L 6-BA+0.1mg / L NAA+0.05mg / L IBA+30g / L sucrose+6.0g / L agar, with a pH of 5.8.

[0037] (2) Rooting culture: Cut the adventitious buds up to 2 cm in step (1) and inoculate them on the rooting medium to induce rooting. After inoculation, first cultivate them in total darkness at 25°C for 3 days, and then place them under light for 12 hours a day , the light intensity is 1500lx, and the cultivation temperature is cultivated until rooting under the condition of 25°C. The rooting medium is: 1 / 2MS+0.1mg / L NAA+20g / L sucrose+4.0g / L agar, pH is 5.8.

[0038] (3) Hard...

Embodiment 2

[0040] (1) Inducing adventitious buds: select the root system of the aseptic seedlings of S. mulberry as explants, cut 2 to 3 cuts to increase the wound; inoculate it on the adventitious bud induction medium in a horizontal manner, at a temperature of 25°C and light intensity 2500lx, and the light time is 15h per day for cultivating until adventitious buds are induced. The adventitious bud induction medium is: 1 / 2MS+1.5mg / L 6-BA+0.05mg / L NAA+0.01mg / L IBA+20g / L sucrose+6.0g / L agar, with a pH of 6.0.

[0041] (2) Rooting culture: Cut the adventitious buds up to 4 cm in step (1) and inoculate them on the rooting medium to induce rooting. After inoculation, first culture them in total darkness at 25°C for 3 days, and then place them under light for 15 hours a day , the light intensity is 2500lx, and the cultivation temperature is cultivated until rooting under the condition of 28° C.; the rooting rate reaches 85%. The rooting medium is: 1 / 2MS+0.1mg / LNAA+15g / L sucrose+4.0g / L agar,...

Embodiment 3

[0044] (1) Inducing adventitious buds: select the root system of the aseptic seedlings of S. mulberry as explants, cut 2 to 3 cuts to increase the wound; inoculate it on the adventitious bud induction medium in a horizontal manner, at a temperature of 28°C and light intensity 1500lx, light time is 15h under the condition of cultivation, until induction to form adventitious buds. The adventitious bud induction medium is: 1 / 2MS+1.5mg / L 6-BA+0.2mg / LNAA+0.1mg / L IBA+25g / L sucrose+6.0g / L agar, with a pH of 6.2.

[0045] (2) Rooting culture: Cut the adventitious buds up to 3 cm in step (1) and inoculate them on the rooting medium to induce rooting. After the inoculation, first cultivate them in the dark at 28°C for 5 days, and then place them in the light for 12 hours every day. , under the condition that the light intensity is 2500lx and the culture temperature is 25°C, it is cultivated until rooting. Described rooting medium is: 1 / 2MS+0.4mg / L NAA+30g / L sucrose+6.0g / L agar, pH is 5...

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Abstract

The invention discloses a high-efficiency one-step regeneration method using the root system of mulberry tree as an explant, which comprises the following steps: S1. Adventitious bud induction: select the root system of a sterile seedling of mulberry tree as an explant, cut out the wound, and inoculate it into On the adventitious bud induction medium, induce the formation of adventitious buds; S2. Rooting culture: cut the adventitious buds in step S1 and inoculate them on the rooting medium to induce rooting, and cultivate the adventitious buds until they take root to form regenerated shoots. This method uses the root system of the mulberry tree as the explant, and successfully obtained the regenerated plant of the tree mulberry in vitro through the steps of induction of adventitious buds, rooting of the adventitious buds, and domestication and transplanting. Compared with the method, the operation is easier, the material is more extensive, and the propagation is more efficient.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-efficiency one-step regeneration method using the root system of a mulberry tree as an explant. Background technique [0002] Broussonetia Papyrifera (L.) Vent., also known as hair peach, mulberry peach, paper mulberry, valley tree, etc., is a perennial deciduous tree belonging to the family Moraceae. The adult tree is 10-20m high; the bark is dark gray; the whole plant contains latex; the branchlets are densely pilose; the leaves are broadly ovate to oblong-ovate and spirally arranged, the apex is acuminate, the base is heart-shaped, and the edge is coarsely serrated. The leaves of small trees often have obvious 3-5 lobes, and the back is densely covered with fluff; the petiole is 2.5-8cm long and densely covered with strigose; flowers are dioecious; the male inflorescence is a catkin, and the female inflorescence is spherical-headed; the fleshy c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 周玮邹金拓林佳娜陈晓阳张冰楠张俊杰阙青敏
Owner SOUTH CHINA AGRI UNIV
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