A high-efficiency one-step regeneration method using mulberry roots as explants

A technology of explants and tree roots, which is applied in the field of plant tissue culture, can solve the problems of low multiplication coefficient and unstable induction rate, etc., and achieve the effects of increasing multiplication coefficient, shortening regeneration time, and convenient material collection
CN113207690BActive Publication Date: 2022-03-11SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2022-03-11

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Abstract

The invention discloses a high-efficiency one-step regeneration method using the root system of mulberry tree as an explant, which comprises the following steps: S1. Adventitious bud induction: select the root system of a sterile seedling of mulberry tree as an explant, cut out the wound, and inoculate it into On the adventitious bud induction medium, induce the formation of adventitious buds; S2. Rooting culture: cut the adventitious buds in step S1 and inoculate them on the rooting medium to induce rooting, and cultivate the adventitious buds until they take root to form regenerated shoots. This method uses the root system of the mulberry tree as the explant, and successfully obtained the regenerated plant of the tree mulberry in vitro through the steps of induction of adventitious buds, rooting of the adventitious buds, and domestication and transplanting. Compared with the method, the operation is easier, the material is more extensive, and the propagation is more efficient.
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Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-efficiency one-step regeneration method using the root system of a mulberry tree as an explant. Background technique

[0002] Broussonetia Papyrifera (L.) Vent., also known as hair peach, mulberry peach, paper mulberry, valley tree, etc., is a perennial deciduous tree belonging to the family Moraceae. The adult tree is 10-20m high; the bark is dark gray; the whole plant contains latex; the branchlets are densely pilose; the leaves are broadly ovate to oblong-ovate and spirally arranged, the apex is acuminate, the base is heart-shaped, and the edge is coarsely serrated. The leaves of small trees often have obvious 3-5 lobes, and the back is densely covered with fluff; the petiole is 2.5-8cm long and densely covered with strigose; flowers are dioecious; the male inflorescence is a catkin, and the female inflorescence is spherical-headed; the fleshy c...

Claims

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