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Rapid propagation method for tissue culture of moellendorf's spidemoss herb

A technology of tissue culture and cypress, applied in the field of plant tissue culture, can solve the problems of trait separation, unstable genetic traits, loss of good traits of parents, etc., and achieve the effect of long cycle, promoting industrialization development, and guaranteeing high-quality seedlings

Inactive Publication Date: 2019-02-15
广西玉林市华睿茶业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Cedarwood seedlings are mainly propagated by seeds and cuttings. Although a large number of seedlings can be obtained in a short period of time by sowing seeds, because Cedarwood is a monoecious cross-pollinated plant, traits are prone to segregation in offspring, and genetic traits are not consistent. Stable, easy to lose the excellent traits of the parent
However, the cutting method requires a large number of branches, and it takes a long time from cutting to transplanting in the field.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Induction of adventitious buds: Select the annual jointed rattans of Dibaizhi as explants, sterilize them with 75% alcohol for 15 seconds, rinse them with sterile water for 6 times, and put them in 0.1% mercuric chloride solution for disinfection. After 18 minutes, wash it with sterile water for 7 times, dry it with sterile filter paper, cut it into 5 cm canes with joints, inoculate it on the induction medium, and culture it in the dark at 25°C for 21 days. It can induce the formation of adventitious buds, the pollution rate is as low as 3%, and the adventitious bud induction rate can reach 93%. The induction medium is: MS+0.3mg / LTDZ+1.5mg / L 6-BA+1.5mg / LNAA+28g / L sucrose+3.5g / L agar, pH is 5.6;

[0013] (2) Subculture: Cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, place them in the light for 16 hours a day after inoculation, the light intensity is 2100 lx, and the culture temperature is 2...

Embodiment 2

[0017] (1) Induction of adventitious buds: Select the annual jointed rattans of Dibaizhi as explants, sterilize them with 75% alcohol for 18 seconds, rinse them with sterile water for 6 times, and put them in 0.5% mercuric chloride solution for disinfection After 18 minutes, rinse with sterile water for 9 times, dry the water through sterile filter paper, cut into 7 cm canes with joints, inoculate them on the induction medium, and culture them in the dark at 26°C for 15 days. It can induce the formation of adventitious buds, the pollution rate is as low as 8%, and the adventitious bud induction rate can reach 96%. The induction medium is: MS+0.6mg / LTDZ+1.8mg / L 6-BA+0.5mg / LNAA+30g / L sucrose+6.0g / L agar, with a pH of 5.7.

[0018] (2) Subculture: cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, and place them in the light for 16 hours per day after inoculation, the light intensity is 2800 lx, and the cul...

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PUM

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Abstract

The invention discloses a rapid propagation method for tissue culture of moellendorf's spidemoss herb. The moellendorf's spidemoss herb is a perennial herb, and is produced in most regions of the south of the Yangtze River, such as Hubei, Shaanxi and Guangxi. The moellendorf's spidemoss herb is grown in mountains, forest shades and other wet places, is sweet, spicy and neutral in nature, and has the functions of stopping bleeding, clearing heat, removing dampness and the like. At present, the seedlings of the moellendorf's spidemoss herb are mainly propagated through seeds and cuttings, whichhave the problems of long cycle, high cost and low efficiency. Therefore, in the method, stem segments with nodes are used as explants, in vitro regrowth plants of the moellendorf's spidemoss herb aresuccessfully obtained by adventitious bud induction, proliferation, rooting, seedling hardening and transplanting and the like, and a rapid propagation technology system for the tissue culture of themoellendorf's spidemoss herb is established. The method is of great significance for the rapid propagationand large-scale promotion of fine varieties and the promotion of industrial development.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of tissue culture of cypress branches. Background technique [0002] Dicypress is a perennial plant, produced in most areas south of the Yangtze River such as Hubei, Shaanxi, and Guangxi. Born in damp places such as mountains and forests, it tastes sweet, pungent, and flat in nature. It has the functions of hemostasis, clearing away heat and promoting dampness. At present, Cedarwood seedlings are mainly propagated by seeds and cuttings. Although a large number of seedlings can be obtained in a short period of time by sowing seeds, because Cedarwood is a monoecious cross-pollinated plant, traits are prone to segregation in offspring, and genetic traits are not consistent. Stable and easy to lose the excellent traits of the parent. The cutting method requires a large number of branches, and it takes a long time from...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈金水肖华洲
Owner 广西玉林市华睿茶业有限公司
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