Method for fast-propagating of brazilian rubber plant own-rooted juvenile form clone by using embryoid

Abstract

The present invention discloses a method for rapid propagation of the scion root young clone of Hevea brasiliensis with embryoid; the method is characterized in that the method comprises an anther embryonic callus inducement process, an embryogenesis process of primary body, a secondary embroid inducement process and a plant regeneration process. The present invention is provided with simple process flow and low production cost; at the same time, the present invention solves the problem of repeated, low-efficiency, arduous physical labor to continuously collect buds or fruit and continuously induce the embryonic callus with traditional method; with the method, people can obtain a large number of ripe embryos fast continuously with low cost and realize the proliferation of embryo; the high-efficiency propagation of the scion root young clone of Hevea brasiliensis is realized and the propagation efficiency of scion root clone is promoted; in this way, the production efficiency of Hevea brasiliensis scion root clone plant in factory is greatly increased.

Description

technical field [0001] The invention relates to the technical field of plant cell engineering, and more specifically relates to a method for rapidly propagating autogenous juvenile clones of Hevea brasiliensis by using embryoid bodies. Background technique [0002] Natural rubber is one of the four major industrial raw materials and an extremely important strategic resource. At present, among more than 2,000 kinds of rubber-producing plants in nature, about 99% of commercial natural rubber comes from Hevea brasiliensis. Hevea brasiliensis is a perennial tall tree belonging to the family Euphorbiaceae, and the conventional breeding cycle is more than 30 years. The ultra-long breeding cycle has greatly restricted the selection and renewal of new varieties of Hevea brasiliensis. In order to broaden the breeding approaches and innovate breeding methods of Brasilia, since the 1950s, breeders from all over the world have begun to study the application of biotechnology in Brasilia...

AI Technical Summary

Problems solved by technology

However, at present, the main problems in the production of self-rooted clones of Hevea brasiliensis are: 1. The acquisition of anthers and inner integuments is limited by seasons, and it is impossible to obtain explants continuously and for a long time; 2. The ability of embryogenesis is limited by genotype. Influence, a system for inducing embryogenesis is the most effective for one or several genotypes, and it is not universally applicable; 3. The current self-rooted clone micropropagation technology has low propagation efficiency

These problems have seriously hindered the large-scale production of autogenous juvenile clones, and thus cannot meet the demand for a large number of autorooted juvenile plants in field production