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Human lung adenocarcinoma self-transferring cell strain and its construction

A technology for human lung adenocarcinoma and method establishment, applied in the direction of tumor/cancer cells, animal cells, vertebrate cells, etc.

Inactive Publication Date: 2007-06-20
SHANGHAI CHEST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are no reports of highly metastatic human lung adenocarcinoma cell lines with spontaneous metastasis and simultaneous invasion of lung, lymph nodes (mediastinal and axillary lymph nodes) and bone tissue in domestic and foreign literature

Method used

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  • Human lung adenocarcinoma self-transferring cell strain and its construction
  • Human lung adenocarcinoma self-transferring cell strain and its construction
  • Human lung adenocarcinoma self-transferring cell strain and its construction

Examples

Experimental program
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Effect test

Embodiment 1

[0033] 1. Pre-experiment human lung adenocarcinoma cells 1×10 6 , injected under the skin of immunodeficiency mice, and when the tumor grew to 0.5-1 cm, the tumor was taken out, and the surrounding tumor tissue was cut into pieces, mashed, and cell cultured.

[0034] 2. Immunodeficiency mice weigh more than 20 grams, regardless of sex. After purchasing, they were housed in SPF cages for several days.

[0035] 3. To anesthetize the experimental animals, inject diluted thiopental sodium (0.75 mg / mouse) and ketamine (1.5 mg / mouse) into the abdominal cavity and leg muscles of the mice respectively. Standby (intravenous injection of cancer cells does not require anesthesia).

[0036] 4. Cancer cell injections were first injected into the left ventricle of anesthetized mice at 0.8 x 10 6 SPC-A-1 human lung adenocarcinoma cells.

[0037] 5. Weigh the next day after two weeks of injection. Those weighing less than 16 grams should be closely observed to prevent death.

[0038] 6...

Embodiment 2

[0044] 1. Spontaneously transfer 2 million cells per BNX mouse, set aside.

[0045] 2. Immunodeficiency mice weighing 18 grams, 6-7 weeks old, both male and female. After purchasing, they were housed in SPF cages for several days.

[0046] 3. Establishment of spontaneous metastasis model Inject 1 million / 0.1 ml of spontaneously transferred cells subcutaneously on the left and right sides of the mouse.

[0047] 4. Measure the size of the tumor. Measure the size of the tumor every week after three weeks. After 45 days, close observation is required to prevent death.

[0048] 5. Imaging Subcutaneous cancer cells were implanted for 60 days for isotope bone scanning. Before scanning, the mice were deeply anesthetized. During the scanning process, some large lung nodules, lymph nodes and bone metastases showed radioactive concentrations, which must be focused on exploration.

[0049] 6. Anatomy: measure the size of the tumor before dissection, and take digital photos of the whole...

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Abstract

A spontaneous transferring cell strain SPC-A-1BM is taken as human-lung adenocarcinoma SPC-A-1 cell strain. It has high spontaneous transferring rate and missed rate, lung transferring rate reaches to 80%, nodus lymphoideus transferring rate reaches to 85% and bone transferring rate reaches to 25%. It has excellent DNA cell stability and can be used to make research for human-lung adenocarcinoma transferring mechanism.

Description

technical field [0001] The invention belongs to the fields of biotechnology and microbial animal cell lines, and specifically relates to a human lung adenocarcinoma cell line with high potential for spontaneous transfer and its establishment method and application. Background technique [0002] Lung cancer is one of the most common and most malignant tumors, ranking among the top three in the incidence and mortality of malignant tumors in the world. In my country, especially large and medium-sized cities, lung cancer has always been the first malignant tumor killer since the 1980s. At present, the principle of clinical treatment of lung cancer is multidisciplinary comprehensive treatment based on surgery. After decades of unremitting efforts, a considerable number of patients have achieved long-term survival through early diagnosis, early treatment and active comprehensive treatment (including surgery, radiotherapy, chemotherapy and biological therapy, etc.), but most patie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N5/09
Inventor 杨顺芳
Owner SHANGHAI CHEST HOSPITAL
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