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Dedicated dispersion liquid for preparing high-resolution chromosome for karyotyping analysis

A technology of karyotype analysis and chromosome, which is applied in the biological field, can solve the problems of increased separation ratio and difficulty in observing the detailed banding of chromosomes, and achieve the effect of improving quality and spreading and dispersing effect

Active Publication Date: 2019-03-29
BEIJING INST OF HUMAN GENETICS & REPRODUCTION MEDICINE LTD CHINA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The duration of hypotonic fluid treatment of cells has a great impact on the chromosome structure and banding effect. If the treatment time exceeds 20 minutes or is too long, the proportion of sister chromatid separation will increase significantly (Ikeuchi, 2004), and it is difficult to observe the chromosome structure. detailed stripes
The purpose of the present invention is to develop a dispersion solution specially used for preparing high-resolution chromosomes, which can not only reduce the overlapping and winding of chromosomes, but also avoid the artificial separation of sister chromatids from affecting the banding analysis.

Method used

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  • Dedicated dispersion liquid for preparing high-resolution chromosome for karyotyping analysis
  • Dedicated dispersion liquid for preparing high-resolution chromosome for karyotyping analysis
  • Dedicated dispersion liquid for preparing high-resolution chromosome for karyotyping analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] High-resolution chromosome dispersion liquid specially prepared for karyotype analysis, characterized in that it consists of the following components: HEPES 1mol / L, pH 8.0; KCl 4g / L, solvent 1L of triple distilled water.

[0055] Wherein, it is prepared from the following raw materials:

[0056] A. Raw material for weighing ratio: KCl 4g;

[0057] B. Dissolve the above raw materials into 1000ml triple distilled water and fully dissolve;

[0058] C. Add 0~1mmol / L HEPES and mix well;

[0059] D. Store at 4°C.

[0060] The dispersion liquid of experimental group 1 is HEPES working concentration is 0.1mmol / L, KCl working concentration is 4g / L.

[0061] The dispersion liquid of experimental group 2 is HEPES working concentration is 0.25mmol / L, KCl working concentration is 4g / L.

[0062] The dispersion liquid of experimental group 3 is HEPES working concentration is 0.5mmol / L, KCl working concentration is 4g / L.

[0063] The dispersion liquid of experimental group 4 is HE...

Embodiment 2

[0083] High-resolution chromosome dispersion liquid specially prepared for karyotype analysis, characterized in that it consists of the following components:

[0084] Sodium hexametaphosphate, pH 8.0, KCl 4g / L, solvent 1L triple distilled water.

[0085] Wherein, it is prepared from the following raw materials:

[0086] A. Raw material for weighing ratio: KCl 4g;

[0087] B. Dissolve the above raw materials into 1000ml triple distilled water and fully dissolve;

[0088] C. Add 0-1mmol / L sodium hexametaphosphate and mix well;

[0089] D. Store at 4°C.

[0090] The dispersion liquid of experimental group 5 is sodium hexametaphosphate working concentration of 0.25mmol / L, and KCl working concentration of 4g / L.

[0091] The dispersion liquid of experimental group 6 is sodium hexametaphosphate working concentration of 0.5mmol / L, and KCl working concentration of 4g / L.

[0092] The dispersion liquid of experimental group 7 was sodium hexametaphosphate with a working concentration...

Embodiment 3

[0114] High-resolution chromosome dispersion solution specially prepared for karyotype analysis, characterized in that it consists of the following components: Tris-HCL 0.05-1 mmol / L, pH 8.0, KCl 4g / L, and solvent 1L of triple-distilled water.

[0115] Wherein, it is prepared from the following raw materials:

[0116] A. Raw material for weighing ratio: KCl 4g;

[0117] B. Dissolve the above raw materials into 1000ml triple distilled water and fully dissolve;

[0118] C. Add 0.05~1mmol / L Tris-HCL and mix well;

[0119] D. Store at 4°C.

[0120] The dispersion liquid of experimental group 9 was Tris-HCl with a working concentration of 0.05mmol / L and KCl with a working concentration of 4g / L.

[0121] The dispersion liquid of experimental group 10 is Tris-HCl working concentration is 0.1mmol / L, KCl working concentration is 4g / L.

[0122] The dispersion liquid of experimental group 11 was Tris-HCl with a working concentration of 0.25mmol / L and KCl with a working concentration ...

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Abstract

The invention relates to a dedicated dispersion liquid for preparing high-resolution chromosome for karyotyping analysis. The dedicated dispersion liquid for preparing high-resolution chromosome for karyotyping analysis is characterized by comprising the following components: 0.05-1mmol / L of Tris-HCL in pH of 7.5 to 10.0 and 2-8g / L of KCl, wherein the solvent is tri-distilled water. The dedicateddispersion liquid for preparing high-resolution chromosome for karyotyping analysis disclosed by the invention has good spread dispersion effect on the lymphocyte chromosome, the intersection and overlapping number between the chromosomes are reduced for the high-resolution and ultra-high-resolution karyotype analysis, and the quality for analyzing the karyotype is greatly improved.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the special preparation of high-resolution chromosome dispersion liquid for karyotype analysis. Background technique [0002] Chromosomes are the carriers of genes. There are 23 pairs of 46 chromosomes in normal human cells, of which 22 pairs and 44 are autosomes, and the other pair is 2 sex chromosomes. Chromosomes may undergo quantitative and structural changes, resulting in various genetic defects, congenital genetic diseases, embryo suspension and spontaneous abortion, tumors, etc. Diseases caused by chromosomal abnormalities are generally called chromosomal diseases. [0003] Chromosomal detection is currently mostly used in clinical practice such as reproduction, obstetrics and gynecology, newborns, blood diseases, tumors and other specialties. Although the method is relatively simple, it is still the gold standard for prenatal diagnosis by using the chromosome banding technique...

Claims

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Application Information

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IPC IPC(8): G01N1/38
CPCG01N1/38
Inventor 李存玺刘恒涛刘舒李能干
Owner BEIJING INST OF HUMAN GENETICS & REPRODUCTION MEDICINE LTD CHINA
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