Chromosome preparation method, as well as required culture medium and preparation method thereof

A culture medium and chromosome technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as unstable quality, affecting disease diagnosis, and lack of uniform standards for culture medium, so as to achieve stable quality and facilitate scientific research and use , easy to check the effect of the analysis

Inactive Publication Date: 2012-05-09
苏州苏大赛尔免疫生物技术有限公司
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there is no uniform standard for the culture medium used in domestic hospitals and laboratories for chromosome examination, and the quality is extremely unstable. Chromosomal analysis cannot be performed, affecting the diagnosis of the disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method for a medium for chromosome preparation, comprising the following steps:

[0046] Step 1, preparing an initial solution, the formulation of the initial solution is as follows:

[0047] RPMI1640: 10g / L, heparin sodium: 7000IU, HEPES: 1.19g / L, L-glutamine: 0.6g / L, NaHCO3: 1g / L, penicillin G potassium: 0.1g / L, streptomycin sulfate: 0.06 g / L, bovine serum: 200ml / L, add water for injection to 1L, stir evenly until a clear solution;

[0048]Step 2, filter and sterilize the above-mentioned initial solution with a filter membrane;

[0049] Step 3, prepare phytohemagglutinin PHA solution, prepare 2% phytohemagglutinin PHA solution with water for injection, and set aside;

[0050] Step 4, medium preparation, adding the phytohemagglutinin PHA solution: 50 mg / L to the filtered initial solution, and stirring evenly to prepare a medium.

[0051] Step 5: Packing

[0052] The solution described in step 4 is dispensed into vials, and sealed with a cap.

[0053]...

Embodiment 2

[0056] A preparation method for a medium for chromosome preparation, comprising the following steps:

[0057] Step 1, preparing an initial solution, the formulation of the initial solution is as follows:

[0058] RPMI1640: 11g / L, heparin sodium: 6400IU, HEPES: 4.05g / L, L-glutamine: 0.5g / L, NaHCO3: 2g / L, penicillin G potassium: 0.05g / L, streptomycin sulfate: 0.1 g / L, bovine serum: 100ml / L, add water for injection to 1L, stir evenly until a clear solution;

[0059] Step 2, filter and sterilize the above-mentioned initial solution with a filter membrane;

[0060] Step 3, prepare phytohemagglutinin PHA solution, prepare 2% phytohemagglutinin PHA solution with water for injection, and set aside;

[0061] Step 4, medium preparation, adding the phytohemagglutinin PHA solution: 200 mg / L to the filtered initial solution, and stirring evenly to prepare a medium.

[0062] Step 5: Packing

[0063] The solution described in step 4 is dispensed into vials, and sealed with a cap.

[006...

Embodiment 3

[0067] A preparation method for a medium for chromosome preparation, comprising the following steps:

[0068] Step 1, preparing an initial solution, the formulation of the initial solution is as follows:

[0069] RPMI1640: 10.5g / L, heparin sodium: 6600IU, HEPES: 3g / L, L-glutamine: 0.55g / L, NaHCO3: 1.5g / L, penicillin G potassium: 0.08g / L, streptomycin sulfate: 0.08g / L, bovine serum: 150ml / L, add water for injection to 1L, stir well until a clear solution;

[0070] Step 2, filter and sterilize the above-mentioned initial solution with a filter membrane;

[0071] Step 3, prepare phytohemagglutinin PHA solution, prepare 2% phytohemagglutinin PHA solution with water for injection, and set aside;

[0072] Step 4, medium preparation, adding the phytohemagglutinin PHA solution: 120 mg / L to the filtered initial solution, and stirring evenly to prepare a medium.

[0073] Step 5: Packing

[0074] The solution described in step 4 is dispensed into vials, and sealed with a cap.

[007...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a chromosome preparation method, as well as a required culture medium and a preparation method thereof, and is used for solving karyotype analysis problem of chromosome. The culture medium consists of RPMI (Roswell Park Memorial Institute) 1640, heparin sodium, HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid), L-glutamine, NaHCO3, benzylpenicillin potassium, streptomycin sulphate, bovine serum and phytohemagglutinin (PHA). The detection method comprises the following steps: implanting 0.3 to 0.4ml of human peripheral blood into the culture medium; adding colchicinamide in 2-4 hours before culture is terminated to realize that the cell is terminated in anaphase; culturing the cell after 68 to 72 hours to harvest the cell; performing hypotonicity for 40 minutes, three times of fixation, banding, dyeing and other treatments; and performing chromosome analysis under a microscope to determine whether the peripheral blood supplier has a phenomenon of chromosome abnormality. The culture medium disclosed by the invention has convenience for use, simpleness in operation, low cost and low patient detection fee, and is suitable for genetic diagnosis, infertility and prenatal diagnosis in each level of hospitals.

Description

technical field [0001] The invention relates to an effective chromosomal molecular reagent material in the fields of human medical genetics and reproductive medicine, in particular to a chromosome preparation method for chromosomal disease diagnosis, a required culture medium and a preparation method thereof. Background technique [0002] Chromosomes are carriers of genetic material, namely genes. There are 46 chromosomes in each human cell, including 44 autosomes and 2 sex chromosomes. Whether it is an autosome or a sex chromosome, they all carry thousands of genes. Therefore, the number of chromosomes or small structural abnormalities will cause the addition or deletion of many genes, resulting in a variety of deformities or abnormal syndromes. Diseases caused by abnormalities in the number or structure of chromosomes are called chromosomal diseases, and chromosomal diseases often exist in the form of syndromes. [0003] Chromosomal disease is a common genetic disease. Wh...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 费敏
Owner 苏州苏大赛尔免疫生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap