40 results about "Benzylpenicillin" patented technology

The invention provides feruloyl esterase and a preparing method and application thereof. A feruloyl esterase gene coming from a soil macro gene library have the nucleotide sequence and amino acid sequence shown in SEQ ID NO.1 and SEQ ID NO.2. The gene contains a tetrapeptide SXXK sequence motif which is rarely seen, and after the esterase gene is inserted into plasmid pET28a(+), the gene is transformed into escherichia coli BL21(DE3) to achieve heterogeneous expression. The molecular weight of purified recombinase (DLFae4) is 38.3 kDa. Besides, it is put forward for the first time that novel feruloyl esterase can hydrolyze penbritin, penicillin, cefazolin and other lactam antibiotics. As is shown by site-directed mutagenesis experiments, a catalysis triplet of DLFae4 is composed of serine(S11), histidine (H74) and aspartic acid (D302), and the mutation of any of serine (S11), histidine (H74) and aspartic acid (D302) can cause loss of the catalysis capability of DLFae4. DLFae4 has a high hydrolytic activity on methyl ferulate and has good heat stability. In the presence of cellulase, DLFae4 can obviously increase the amount of ferulic acid released from destarched wheat bran. Due to peculiar activities and enzymatic characteristics of novel feruloyl esterase, novel feruloyl esterase can be applied to feed, paper making, food, pharmacy and other fields.

The present invention relates to a compound injection preparation formed from probenecid sodium and benzylpenicillin antibiotics, belonging to the field of chemical pharmaceutical technology. The benzylpenicillin antibiotics include amoxicillin sodium and piperacillin sodium. The amoxicillin sodium and piperacillin sodium are mixed according to ratio of 1:3 and 1:5 through a conventional preparation process.

The invention relates to an electrochemical biosensor which detects ampicillin without enzyme based on a nucleic acid aptamer, which belongs to the technical field of electrochemical biosensors. According to the invention, ampicillin is detected based on target-induced conformational changes in the nucleic acid aptamer and catalytic hairpin self-assembly amplification (CHA) and strand replacementstrategies, which achieves multiple signal amplification, reduces detection limits, and improves sensitivity; gold electrodes are simple, compact, easy to carry, and can be used multiple times; simpleand sensitive detection of a target object is realized; the electrochemical biosensor has the advantages of simple preparation method, stable performance and good electrode repeatability; the reaction process does not require the participation of enzyme, which greatly reduces the cost; and the electrochemical biosensor is suitable for the practical application of ampicillin detection and biosensor industrialization in food safety.

The invention relates to the technical field of biosensors, especially relates to a fluorescent biosensor based on hybrid chain reaction amplification, and solves the problems in the prior art that the method for detecting ampicillin is low in specificity and sensitivity, and the cost is high. According to a biosensor for detecting the ampicillin based on an aptamer, a cyclic amplification effectis realized by the cooperation of Nb.BbcCI and a chain hybrid chain reaction, and Thioflavine-T and G-quadruplex are combined to generate fluorescence and a homogeneous reaction mixed solution. The preparation method comprises the following steps of preparing gold nanoparticles; modifying Walker and Track to the surfaces of the gold nanoparticles; mixing a labeled nanogold solution with the homogeneous reaction solution; performing hyperbranched hybrid chain reaction and fluorescent detection; carrying out high specificity detection on the target ampicillin by using the aptamer by utilizing the specific recognition of the aptamer; and using hyperbranched hybrid chain reaction amplification to achieve signal amplification.

The invention prepares a thiourea compound. The thiourea compound is prepared by the following steps: dissolving a substrate 5-isothiocyanate methyl isoquinoline into anhydrous dichloromethane or anhydrous tetrahydrofuran, adding different aromatic ring substituted thiazole compounds which is equivalent to 1.1 times the weight of the substrate and diisopropylethylamine which is equivalent to 3-5 times the weight of the substrate, stirring at the room temperature for 3-5 hours; after TLC detection reaction is finished, adding ethyl acetate for dilution, washing twice with 5% diluted hydrochloric acid, washing twice with saturated sodium carbonate, washing twice with water, and washing with saturated table salt water; and drying an organic phase with anhydrous sodium sulfate, carrying out reduced pressure concentration, separating through silica gel column chromatography, so as to obtain the target product. The compound has application values in antibacterial aspects and particularly has better antibacterial effects on inhibiting gram-positive bacteria such as staphylococcus aureus than a known antibacterial drug, namely penbritin, so that the thiourea compound can be taken as a novel antibacterial drug to be researched and utilized.

An antibacterial composite medicine easy to dissolve is prepared from furbenicillin or its physiologically acceptable medicinal salt or hydrate, and the pharmacologically acceptable inorganic salt of water-soluble alkali metal or alkali-earth metal, organic alkali and organic acid or its salt. Its preparing process is also disclosed.

The invention discloses a fermenting technology for improving expression amount of fusion protein of recombinant human brain natriuretic peptide. The fermenting technology comprises the following steps of adding 18 to 20L of BHD culture medium, 65 to 80mL of glycerin, and ampicillin into a fermenting tank until the final concentration is 100mg/L; inoculating, adjusting the air ventilation amount until the dissolved oxygen content in the fermenting liquid is 30% to 50%, adjusting the pH (potential of hydrogen) value to 6.8 to 7.2, and culturing at the temperature of 37 DEG C; when OD600 is 10 to 15, adding isopropyl thiogalactoside until the final concentration is 0.5 to 1.0mmol/L, inducing, adding 150 to 300mL/A of supplementing liquid A into the fermenting tank, stopping fermenting culture after 3 to 6h, and harvesting bacteria. The fermenting technology has the advantages that the expression level of rhBNP fusion protein is obviously improved, the expression amount reaches up to 34.5% to 38.3%, and the good commercial development value and great success in commerce are obtained.

The invention discloses a preparing method of benzathine penicillin which comprises the following procedures: a, 50 percent to 75 percent of ethanol is added to a raw medicine of the N, N-dibenzyl ethylenediamine diacetate, and is prepared into solvent A; b, 50 percent to 75 percent of ethanol is added to the raw medicine of penicillin G salt, and is prepared into solvent B; c, the pre-applied solvent A is added to the solvent B for stirring, then the residual solvent A is added to the solvent B, thus obtaining nzathine benzylpenicillin suspension; d, filtering and then washing and drying are carried out on the nzathine benzylpenicillin suspension. The method has the advantages of improving yield coefficient of products, purity quotient and the controllability of particle size, reducing the toxicity of the residual solvent, improving the quality and safety of products and avoiding the harm of methanol to environment and human body at the same time.

The invention provides a method for rapidly separating purified heterotrophic microalgae from natural water areas by using a combined bacteriostat. The invention is characterized in that the method comprises the steps as follows: a) preparing microalgae autotrophic liquid nutrient medium; b) preparing microalgae heterotrophic solid medium (adding 1% of glucose and 1.5-2% of agar into the microalgae autotrophic liquid nutrient medium); c) preparing a medium plate containing the combined bacteriostat (20-60mu g/mL of ampicillin and 200-600mu g/mL of Natamycin); d) coating natural water sample or propagated and cultured water sample on the medium plate containing the combined bacteriostat, and then culturing in a constant-temperature incubator at the temperature of 25-35 DEG C; e) after colorful algae grows on the plate, using an inoculating loop to pick the colorful algae to the blank medium plate containing the combined bacteriostat, lining and then culturing the medium plate with the algae in the constant-temperature incubator at the temperature of 25-35 DEG C; and f) repeating the step e) for 3-5 times, and then obtaining the purified heterotrophic microalgae. The method is simple, convenient, rapid and reliable in operation and also can separate and purify the heterotrophic microalgae from the natural waters within a shorter time.

A therapeutic formulation comprising a microemulsion of a therapeutic agent in free and/or conjugatively coupled form, wherein the microemulsion comprises a water-in-oil (w/o) microemulsion including a lipophilic phase and a hydrophilic phase, and has a hydrophilic and lipophilic balance (HLB) value between 3 and 7, wherein the therapeutic agent may for example be selected from the group consisting of insulin, calcitonin, ACTH, glucagon, somatostatin, somatotropin, somatomedin, parathyroid honnone, erythropoietin, hypothalamic releasing factors, prolactin, thyroid stimulating hormones, endorphins, enkephalins, vasopressin, non-naturally occurring opioids, superoxide dismutase, interferon, asparaginase, arginase, arginine deaminease, adenosine deaminase, ribonuclease, trypsin, chymotrypsin, papain, Ara-A (Arabinofuranosyladenine), Acylguanosine, Nordeoxyguanosine, Azidothym id ine, Didesoxyadenosine, Dideoxycytidine, Dideoxyinosine Floxuridine, 6-Mercaptopurine, Doxorubicin, Daunorubicin, or I-darubicin, Erythromycin, Vancomycin, oleandomycin, Ampicillin; Quinidine and Heparin. In a particular aspect, the invention comprises an insulin composition suitable for parenteral as well as non-parenteral administration, preferably oral or parenteral administration, comprising insulin covalently coupled with a polymer including (i) a linear polyalkylene glycol moiety and (ii) a lipophilic moiety, wherein the insulin, the linear polyalkylene glycol moiety and the lipophilic moiety are conformationally arranged in relation to one another such that the insulin in the composition has an enhanced in vivo resistance to enzymatic degradation, relative to insulin alone. The microemulsion compositions of the invention are usefully employed in therapeutic as well as non-therapeutic, e.g., diagnostic, applications.

The invention discloses an orixine derivative and an application of orixine derivative in preparation of medicine for resisting drug-fast bacteria, the test shows that the orixine derivative is capable of inhibiting breeding of a plurality of drug-fast bacterium in vitro, and can be used for preparing antibiotic medicines for resisting drug-fast bacteria; the orixine derivative is used for preparing medicines for resisting methicillin-resistant staphylococcus aureus (MRSA), preparing medicines for resisting benzylpenicillin-resistant staphylococcus aureus, and preparing medicines for resisting vancomycin-resistant enterococcus; the medicines can be prepared to the injection, tablet, pill, capsule, a suspending agent or an emulsion for usage, a chemical structural formula of the orixine derivative is shown in formula I, wherein R1,R2 and R3 are defined in a specification.

The invention provides a sterile treatment method of heterosigma akashiwo. The sterile treatment method comprises the following steps of (1) weighing ampicillin, adding sterile water, and uniformly mixing and dissolving to obtain an ampicillin solution; filtering the obtained ampicillin solution by using a filter membrane; (2) preparing a to-be-degermed heterosigma akashiwo culture solution; (3) adding the filtered ampicillin solution into the to-be-degermed heterosigma akashiwo culture solution, and then carrying out sterile culture; and (4) in the sterile culture process, regularly carryingout expanding culture on the heterosigma akashiwo culture solution, and adding the ampicillin solution during expanding culture. According to the method, antibiotic treatment is combined with a platecolony counting method to obtain a relatively sterile heterosigma akashiwo culture, and the long-term treatment of ampicillin can improve the cell viability of heterosigma akashiwo. Considering from the perspectives of economy and environmental protection, the method is suitable for selecting ampicillin with low final concentration for sterile treatment, so that the algae culture is maintained ina relatively sterile state.

The invention relates to paracoccus and cell fraction capable of degrading benzylpenicillin as well as a composition of the paracoccus and the cell fraction. The paracoccus is preserved in China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.12494. The paracoccus and cell fraction capable of degrading the benzylpenicillin as well as the composition of the paracoccus and the cell fraction, provided by the invention, have a capability of efficiently degrading the benzylpenicillin, can be used for carrying out harmless treatment on benzylpenicillin waste mycelia, and have the advantages of greenness and environment friendliness, energy source saving and cost saving.

The invention relates to a culture medium for acquiring and isolating conidia of isaria fumosorosea in greenhouses and a method for preparing the culture medium, and belongs to the field of technologies for isolating and culturing microorganisms. The culture medium for acquiring and isolating the conidia of the isaria fumosorosea in the greenhouses comprises, by weight, 15-20% of potatoes, 1.5-2.0% of sucrose, 25-100 micro-g/ml of ampicillin, 25-100 micro-g/ml of hygromycin B, 1.0-3.0% of agar and the balance distilled water. A pH (potential of hydrogen) value of the culture medium is 7.0-10.0. The culture medium and the method have the advantages that the conidia of the isaria fumosorosea can be acquired and quickly isolated by the culture medium, the quantities of target bacterial colonies on flat plates can be detected, and accordingly diffusion conditions of the conidia of the isaria fumosorosea in control regions can be detected and quantitatively evaluated; the culture medium is high in isolation speed and detection efficiency, and scientific bases can be provided for formulating pest control strategies.

An isolated DNA encoding phenyl acetyl-CoA-ligase and a process of increasing the production of penicillin G in a strain of Penicillium chrysogenum by transforming the strain with the isolated DNA. Also vectors and host organisms having the isolated DNA.

Ampicillin is produced in a batch process by enzymatic acylation of 6-aminopenicillanic acid (6-APA) with the aid of phenylglycine derivative such as D-phenylglycine amide. High conversions of phenylglycine derivative may be achieved by having the total concentration in the reaction mixture of 6-APA and ampicillin greater than 250 mM and the molar ration of total quantity of phenylglycine derivative to total quantity of 6-APA less than 2.5. Higher yields of ampicillin may be achieved when the amount of dissolved 6-APA is kept low, e.g. below 300 mM.

The present invention relates to mutants of lactic acid bacteria which are resistant to the antibiotic ampicillin and which were found to give an increased texture when grown in milk while maintaining the other growth properties of the parent strain. The present invention, furthermore, relates to compositions comprising such mutants, and to dairy products fermented with the lactic acid bacteria resistant to ampicillin.

The invention provides a method for screening spiral seaweeds through gene transformation, and relates to genetic engineering. The method comprises the steps that (1) a spiral seaweed integration and screening plasmid pEGFP-IS-IS-Km containing two inserting sequences, a gfp gene, a marker gene npt II and a marker gene Ap is established, (2) ultrasonic conversion and screening are conducted on a donor plasmid pEGFP-IS-IS-Km, wherein enzyme digestion is conducted on the pEGFP-IS-IS-Km through EcoRI, a linear plasmid is obtained, the two ends of the linear plasmid are each provided with an IS sequence, the linear plasmid is similar to the characteristic structure of a transposon, the spiral seaweed 869s is converted through a laboratory ultrasonic conversion method, benzylpenicillin and G418 (nptII) are used for selecting out spiral seaweed strains with insertion mutations, mutant strains are selected out through a two-step screening method, a resistant plate is used for primary screening, after gradient dilution is completed, a fluorescent microscope is used for picking out singular green fluorescent seaweeds, then ultrasonic secondary screening is conducted, enlarge cultivation is carried out, and seaweed strains with excellent performance are selected out.

The double function cos carrier suitable for streptomycede chromosome gene knock-out features that it consists of following DNA elements: colibacillus plasmid Coie1 duplicating initiation site ori, ampicilin resistance gene bla, streptomycete plasmid pIJ101 duplicating initiation site and duplicon, thiactin resistance gene tsr, strong streptomycete plasmid pIJ101 incompatibility area sti, lambda bacteriophage cos site, RP4 conjugal transfer start site oriT, promoter capable of being identified specifically by T3 and T7 bacteriophage RNA polymerase, and neomycin resistance gene neo from Tn5 and containing no promoter. The present invention may be used easily in knocking out streptomycede chromosome gene, contains colibacillus plasmid, streptomycete plasmid duplicon and resistance screening mark simultaneously and has functions of autonomous duplicating and inheriting simultaneously in two kinds of hosts.

The invention provides a special selenium-rich fertilizer for field crops. The special selenium-rich fertilizer is high in yield and high in plant disease resistance, and comprises, by weight, 26.5-46.2% of small-particle urea, 7-17.1% of ammonium bicarbonate, 6-55.0% of monoammonium phosphate, 30-58.0% of diammonium phosphate, 60% of potassium chloride, 28% of benzylpenicillin sotassium, 60% of clay I, 1-2% of inorganic selenium salt and 2% of organic trace elements.

The invention relates to a tazobactam ampicillin amide composite, its preparation method and application. The tazobactam ampicillin amide composite has a structural formula (I) as the following. The preparation method of the composite comprises: in the presence of dicyclohexycarbodiimide (DCC) and N-hydroxysuccinimide (NHS), carrying out an acylation reaction on tazobactam and ampicillin trihydrate, thus obtaining the tazobactam ampicillin amide composite (I). The tazobactam ampicillin amide composite can be used for treating swine acute bacterial infections.