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Sterile treatment method of heterosigma akashiwo

A technology for the treatment of Heteroflexus albicans is applied in the directions of microorganism-based methods, biochemical equipment and methods, and single-cell algae, which can solve the problems of high cost and only use of physical methods, and achieve good antibacterial effect and maintenance. Vitality and high eco-friendliness

Active Publication Date: 2021-02-05
JIANGSU OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the physical method is expensive and can only be used for emergency treatment in the primary stage, and the irradiation method may kill microorganisms and algae cells at the same time

Method used

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  • Sterile treatment method of heterosigma akashiwo
  • Sterile treatment method of heterosigma akashiwo
  • Sterile treatment method of heterosigma akashiwo

Examples

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Embodiment 1

[0028] A method for aseptic treatment of Heterocurvium akashiwo, comprising the following steps:

[0029] (1) Weigh ampicillin, add sterile water to mix and dissolve to obtain ampicillin solution, the mother liquor concentration of ampicillin solution is 200mg / mL; filter gained ampicillin solution through a filter membrane of 0.22 μm, and pack in 1.5mL centrifuge tube for later use.

[0030] (2) Prepare the Heterocurvium akashiwo culture solution to be sterilized, which specifically includes the following steps: take the stock solution of the Heterocurvium akashiwo culture solution, use the Sedgewick-Rafter Counting Cell counting plate to carry out the algae cell count, determine the concentration of the algae cells in the stock solution, and use 5000cells The inoculum size per mL was the initial concentration for algae expansion to obtain the H. akashiwo culture solution to be sterilized.

[0031] (3) Add the filtered ampicillin solution into the H. akashiwo culture system t...

Embodiment 2

[0035] A method for aseptic treatment of Heterocurvium akashiwo, comprising the following steps:

[0036] (1) Weigh ampicillin, add sterile water to mix and dissolve to obtain ampicillin solution, the mother liquor concentration of ampicillin solution is 200mg / mL; filter gained ampicillin solution through a filter membrane of 0.22 μm, and pack in 1.5mL centrifuge tube for later use.

[0037] (2) Prepare the Heterocurvium akashiwo culture solution to be sterilized, which specifically includes the following steps: take the stock solution of the Heterocurvium akashiwo culture solution, use the Sedgewick-Rafter Counting Cell counting plate to carry out the algae cell count, determine the concentration of the algae cells in the stock solution, and use 5000cells / mL inoculum was the initial concentration for expansion to obtain the H. akashiwo culture solution to be sterilized.

[0038] (3) Add the filtered ampicillin solution to the culture medium of H. akashiwo to be sterilized. ...

Embodiment 3

[0042] A method for aseptic treatment of Heterocurvium akashiwo, comprising the following steps:

[0043] (1) Weigh ampicillin, add sterile water to mix and dissolve to obtain ampicillin solution, the concentration of the mother liquor of ampicillin solution is 200 mg / mL; filter the ampicillin solution through a 0.22 μm filter membrane, and pack Store in a 1.5mL centrifuge tube for later use.

[0044](2) Prepare the Heterocurvium akashiwo culture solution to be sterilized, which specifically includes the following steps: take the stock solution of the Heterocurvium akashiwo culture solution, use the Sedgewick-Rafter Counting Cell counting plate to carry out the algae cell count, determine the concentration of the algae cells in the stock solution, and use 5000cells / mL inoculum was the initial concentration for expansion to obtain the H. akashiwo culture solution to be sterilized.

[0045] (3) Add the filtered ampicillin solution into the H. akashiwo culture medium to be ster...

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Abstract

The invention provides a sterile treatment method of heterosigma akashiwo. The sterile treatment method comprises the following steps of (1) weighing ampicillin, adding sterile water, and uniformly mixing and dissolving to obtain an ampicillin solution; filtering the obtained ampicillin solution by using a filter membrane; (2) preparing a to-be-degermed heterosigma akashiwo culture solution; (3) adding the filtered ampicillin solution into the to-be-degermed heterosigma akashiwo culture solution, and then carrying out sterile culture; and (4) in the sterile culture process, regularly carryingout expanding culture on the heterosigma akashiwo culture solution, and adding the ampicillin solution during expanding culture. According to the method, antibiotic treatment is combined with a platecolony counting method to obtain a relatively sterile heterosigma akashiwo culture, and the long-term treatment of ampicillin can improve the cell viability of heterosigma akashiwo. Considering from the perspectives of economy and environmental protection, the method is suitable for selecting ampicillin with low final concentration for sterile treatment, so that the algae culture is maintained ina relatively sterile state.

Description

technical field [0001] The invention relates to the technical field of marine microalgae, in particular to a method for aseptically treating Heterocurvium akashiwo. Background technique [0002] In the related research of marine microalgae, it is especially required that the relevant algae strains in the experiment must be sterile algae strains. Bacteria and microalgae may have a symbiotic relationship. In the heterotrophic medium of microalgae, bacteria will grow rapidly and compete with microalgae for nutrients in the medium to limit the growth of microalgae. The aseptic state of the microalgae culture system is a necessary prerequisite for the study of the utilization of organic matter by microalgae, because the organic matter decomposed by bacteria may be used by algae, which will cause large errors in the research results. Therefore, the relative aseptic treatment in the microalgae culture system has become a key step and basis for microalgae-related research. In orde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12Y02A40/80
Inventor 胡广伟张珍珍陈磊徐凯姬南京申欣
Owner JIANGSU OCEAN UNIV
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