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Paracoccus and cell fraction capable of degrading benzylpenicillin as well as composition of paracoccus and cell fraction

A paracoccus, penicillin technology, applied in the direction of bacteria, microorganisms, microorganisms, etc., to achieve efficient degradation of penicillin, cost saving, energy saving effect

Active Publication Date: 2017-06-13
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no feasible, green and environmentally friendly method for the harmless treatment of penicillin production fungus residues, so the harmless treatment of antibiotic mycelia has become a major problem in related industries

Method used

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  • Paracoccus and cell fraction capable of degrading benzylpenicillin as well as composition of paracoccus and cell fraction
  • Paracoccus and cell fraction capable of degrading benzylpenicillin as well as composition of paracoccus and cell fraction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Screening, Identification, Degradability and Sequence Determination of Paracoccus Strains

[0029] Step 1: Collect 10 g of sludge from the surrounding area of ​​an antibiotic enterprise, inoculate it into 100 mL of inorganic salt medium containing 30 mg of penicillin, and culture it on a shaking table at 30°C and 160 rpm. Transfer once every 7 days after the start of culture, and transfer to a higher concentration of penicillin-containing medium with a 10% (V / V) inoculum during transfer. The concentration of penicillin is 10mg / L, 30mg / L, 60mg / L, 90mg / L, 120mg / L and so on, to 900mg / L, to obtain different concentrations of enriched culture solution containing bacteria.

[0030] Wherein, the composition and the proportioning ratio of the inorganic salt culture medium among the present invention are (g / L): K 2 HPO 4 1.6g, KH 2 PO 4 0.4g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.025g, FeCl 3 ·6H 2 O 0.0023g, NH 4 NO 3 0.5g, 20.0g agar, adjust the pH val...

Embodiment 2

[0041] Example 2: Screening and identification of paracoccus strains and determination of their degradation ability to penicillin

[0042] Step 1: Take 10g of sludge and put it in a sterilized 250mL Erlenmeyer flask, add 100mL of penicillin solution with a concentration of 0.3g / L, and culture on a shaker at 30°C with a rotation speed of 160r / min. After the culture starts, transfer once every 7 days, inoculate into fresh penicillin solution acclimation medium with 10% (V / V) inoculum amount during transfer, and gradually increase the content of penicillin. Detect the degradation rate of penicillin, and select the best group for the isolation of strains.

[0043] Step 2: Insert the bacterial suspension (1×108 / mL) of the above-mentioned bacterial strains in 1 mL into 100 mL of tap water containing 30 mg / L penicillin, take three parallels, and use tap water without inoculation of bacteria but containing the same concentration of penicillin For control. Cultivate on a shaker at 30...

Embodiment 3

[0047] Embodiment 3: Degradation ability determination of paracoccus bacterial strain to penicillin in the bacteria residue

[0048] Pass the penicillin waste wet mycelium through a 0.2mm sieve, weigh 10g of the sieved waste mycelium, put it into 100mL of water, add 6g of Paracoccus thallus obtained after 48 hours of cultivation to the waste wet mycelia suspension solution at 30°C (wet weight), 3 groups of parallel experiments were set up, taking the addition of penicillin mycelia but not adding paracoccus thalline as contrast, respectively acting on samples for 0, 1, 2, 3, 4 hours, and detecting the residual amount of penicillin by high performance liquid phase method. The results showed that the penicillin degradation rate of the strain could reach 100% after being cultivated for 4 hours.

[0049] Table 2 Degradation effect of KDSPL-02 strain on penicillin in bacteria residue

[0050]

[0051] The beneficial effects of the present invention are:

[0052] 1. The penicill...

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Abstract

The invention relates to paracoccus and cell fraction capable of degrading benzylpenicillin as well as a composition of the paracoccus and the cell fraction. The paracoccus is preserved in China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.12494. The paracoccus and cell fraction capable of degrading the benzylpenicillin as well as the composition of the paracoccus and the cell fraction, provided by the invention, have a capability of efficiently degrading the benzylpenicillin, can be used for carrying out harmless treatment on benzylpenicillin waste mycelia, and have the advantages of greenness and environment friendliness, energy source saving and cost saving.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a paracoccus capable of degrading penicillin, a cell fraction and a composition thereof. Background technique [0002] Penicillin (Benzylpenicillin) is a general term for a family of antibiotics. It inhibits the synthesis of the cell wall by inhibiting the combination of the tetrapeptide side chain and the pentapeptide cross-linking bridge of the bacterial cell wall. It is a broad-spectrum anti-infective drug. [0003] At present, the industrial production of penicillin mainly uses starch, corn steep liquor, bean cake powder, etc. as raw materials, and is fermented, extracted and purified by Penicillium chrysogenum. After the fermented liquid is separated from the solid and liquid to obtain the penicillin aqueous solution, the remaining solid waste residue is the penicillin waste hyphae. The main components of penicillin waste mycelium are crude protein, cellulose,...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/26A62D101/28
CPCA62D3/02A62D2101/26A62D2101/28C12N1/20C12N1/205C12R2001/01
Inventor 刘守信王鹏刘文会王晓春黄净杨毅华
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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