Culture medium for acquiring and isolating conidia of isaria fumosorosea in greenhouses and method for preparing culture medium

A technology of fume-coloured I. spp. and conidia, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., to achieve rapid growth, high detection efficiency, and rapid separation

Active Publication Date: 2017-07-25
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current method for monitoring the conidia o

Method used

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  • Culture medium for acquiring and isolating conidia of isaria fumosorosea in greenhouses and method for preparing culture medium
  • Culture medium for acquiring and isolating conidia of isaria fumosorosea in greenhouses and method for preparing culture medium
  • Culture medium for acquiring and isolating conidia of isaria fumosorosea in greenhouses and method for preparing culture medium

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preparation example Construction

[0036] The present invention also provides a preparation method for the culture medium described in the above technical scheme, comprising the following steps:

[0037] 1) Potatoes are mixed with distilled water, filtered after boiling to obtain potato filtrate;

[0038] 2) the potato filtrate is mixed with sucrose and agar, and sterilized;

[0039] 3) When the sterilized medium is cooled to 55°C, mix it with ampicillin and hygromycin B, and then adjust the pH value to obtain a medium for detecting and isolating conidia of I. .

[0040] The invention mixes potatoes with distilled water, boils and filters to obtain potato filtrate. In the present invention, the boiling time is 20-30 minutes, more preferably 25 minutes. In the present invention, the filtration is gauze filtration, more preferably double-layer gauze filtration.

[0041] After obtaining the potato filtrate, the present invention mixes the potato filtrate with sucrose and agar, and sterilizes. In the present i...

Embodiment 1

[0055] Recipe: Potato 200g, sucrose 20g, hygromycin B 100ug / ml, ampicillin 100ug / ml, agar 10g, add distilled water to 1000mL

[0056] Preparation:

[0057] 1) Take fresh potatoes, wash and peel them, weigh the quality described in the formula, add distilled water to 990mL, boil for 20 minutes, and filter with double-layer gauze;

[0058] 2) Add sucrose and agar of the quality described in the formula to the filtrate, and make up the amount of distilled water to 990mL after boiling;

[0059] 3) Put it into a high-pressure fire pot at a temperature of 121°C, keep it for 30 minutes, and then cool it down naturally;

[0060] 4) When the sterilized culture medium was cooled to 55°C, 100ug / ml of newly prepared ampicillin and hygromycin B solutions of corresponding mass percentage concentrations were added to 990mL culture medium to adjust the pH value to 7.0.

Embodiment 2

[0062] Recipe: Potato 150g, sucrose 16g, hygromycin B 30ug / ml, ampicillin 30ug / ml, agar 10g, add distilled water to 1000mL

[0063] Preparation:

[0064] 1) Take fresh potatoes, wash and peel them, weigh the quality described in the formula, add distilled water to 990mL, boil for 20 minutes, and filter with double-layer gauze;

[0065] 2) Add sucrose and agar of the quality described in the formula to the filtrate, and make up the amount of distilled water to 990mL after boiling;

[0066] 3) Put it into a high-pressure fire pot at a temperature of 121°C, keep it for 30 minutes, and then cool it down naturally;

[0067] 4) When the sterilized culture medium was cooled to 55° C., 990 mL of culture medium was added with 30 ug / ml of newly prepared ampicillin and hygromycin B solutions of corresponding mass percentage concentration, and the pH value was adjusted to 8.0.

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Abstract

The invention relates to a culture medium for acquiring and isolating conidia of isaria fumosorosea in greenhouses and a method for preparing the culture medium, and belongs to the field of technologies for isolating and culturing microorganisms. The culture medium for acquiring and isolating the conidia of the isaria fumosorosea in the greenhouses comprises, by weight, 15-20% of potatoes, 1.5-2.0% of sucrose, 25-100 micro-g/ml of ampicillin, 25-100 micro-g/ml of hygromycin B, 1.0-3.0% of agar and the balance distilled water. A pH (potential of hydrogen) value of the culture medium is 7.0-10.0. The culture medium and the method have the advantages that the conidia of the isaria fumosorosea can be acquired and quickly isolated by the culture medium, the quantities of target bacterial colonies on flat plates can be detected, and accordingly diffusion conditions of the conidia of the isaria fumosorosea in control regions can be detected and quantitatively evaluated; the culture medium is high in isolation speed and detection efficiency, and scientific bases can be provided for formulating pest control strategies.

Description

technical field [0001] The invention relates to the technical field of microorganism isolation and cultivation, in particular to a culture medium for conidia of I. fumigatus for collection and separation in greenhouses and a preparation method thereof. Background technique [0002] The occurrence of insect epidemic diseases requires three important factors: insects, pathogenic microorganisms, and suitable environmental conditions. The three interact and restrict each other. The prevalence of insect diseases mainly depends on the pathogenicity, viability and infectivity of pathogenic microorganisms, as well as suitable climatic conditions. At the same time, the type, density and disease resistance of host insects are also major influencing factors. At present, the research on the epidemic of biocontrol bacteria mainly releases the spores of biocontrol bacteria to spread among the target pest populations to reduce the population rate. After the outbreak of the epidemic, the b...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12Q1/24C12R1/645
CPCC12Q1/045C12Q1/24G01N2333/37
Inventor 郑宇何玉仙刘晓菲姚凤銮丁雪玲翁启勇
Owner INST OF PLANT PROTECTION FAAS
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