151 results about "Anserine" patented technology

The invention provides a fast cultivation method for Taisui, and relates to a Taisui strain cultivation technique and a Taisui health product. A culture medium is made from the following raw materials: mineral water, honey, rhodiola, cordyceps sinensis, astragalus roots, snow lotuses, saffron, cynomorium songaricum, cistanche salsa, potentilla anserine and straight ladybell roots. When the culture medium is made, all materials except honey are cleaned, aired, ground, soaked in mineral water for 24 to 48 hours, subjected to residue filtration, added with honey and prepared into the Taisui culture medium. The method has the following significant effects that: 1, the Taisui is fast to cultivate and can grow by over 1 to 1.5 times in a month; 2, the Taisui is good in color and luster, red, bright and attractive; 3, the Taisui is good in shape and can harden and take shape in a month by the method, while the Taisui cultivated by general culture methods is soft, takes no shape, is layered and needs more than one year to harden; and 4, the Taisui is good in quality, is cultured by adopting nutrient-rich culture medium, and has excellent quality.

The invention provides a feed for breeding Cordyceps sinensis host larvae, and a method for breeding the host larvae, wherein the feed comprises Chinese artichoke and Potentilla anserine L. In some embodiments, the feed also comprises carrots. The invention also provides a method for preparing the feed, and a method for breeding bat moth larvae through adopting the feed. The feed solves an artificial technological link for breeding the bat moth larvae, can be used for the artificial scale breeding of the bat moth larvae, and has a large promotion effect on the artificial cultivation of Cordyceps sinensis, so large economic benefits can be generated.

The invention relates to a method for preparing seleno-polysaccharide by an organic method, which comprises the following steps: (1) placing potentilla anserine polysaccharide and artemisia seed polysaccharide into a reactor, adding N,N-dimethyl formamide into the reactor, and stirring to obtain suspension A; (2) dripping thionyl chloride into selenious acid for reacting, carrying out reduced pressure distillation on the reacted solution to obtain solid matter B; (3) dissolving the solid matter B with the N,N-dimethyl formamide, and adding the mixed solution into the suspension A for reaction to obtain reaction solution C; and (4) dissolving the reaction solution C with distilled water, adjusting the pH value to be 7 with sodium hydroxide, carrying out centrifugation, filling supernatant fluid into a bag filter with the interception quantity of 8,000 to 140,000Da, introducing tap water for dialysis, and carrying out the dialysis with deioned water; and carrying out reduced pressure distillation on the solution in the bag filter, adding ethanol into concentrated solution, standing the mixed solution at room temperature, carrying out centrifugation, collecting deposition on the lower layer and freezing and drying to finally obtain the potentilla anserine polysaccharide or the artemisia seed polysaccharide. The method has the advantages of short reaction time, few byproducts, simple reaction process, high selenium content of products and the like.

The invention relates to a pharmaceutical composition for rapidly reducing content of uric acid in individual blood, which comprises anserine in an amount larger than 50 mg to about 600 mg. the invention further relates to a package that comprises the pharmaceutical composition including anserine, and tags and / or inserts indicative of administering the composition to individual to offer anserine in an amount larger than 50 mg to about 600 mg per dosage. In addition, the invention relates to uses of anserine in the manufacturing of medicines for rapidly reducing content of uric acid in individual blood, wherein the medicine includes anserine in an amount larger than 50 mg to about 600 mg.

A food for improving motor function comprising as effective components carnosine and / or anserine and a material for improving motor performance (a material for motor energy source, a material for promoting conversion into energy and / or an antioxidant) is provided. In the invention, by synergistic effect of these components, the motor function or stamina can be improved and recovery from fatigue can be promoted in humans and mammals. Further, any of the effective components of the food for improving motor function of the invention is a naturally occurring component, therefore, the food has a characteristic of high safety.

Among the fewer than 10 proteins primarily secreted by the species Lactobacillus plantarum, there is one of 30 kDa that contains an internal fragment without cleavage sites for the most important intestinal proteases and characterized by having a serine and threonine content of at least 50%. The genetic information encoded in this fragment, designated ST peptide, has been used for producing and purifying said peptide, thus making it possible to conduct various tests in vitro.To summarize, the ST peptide is considered to promote the process of immunologic ignorance of our gastrointestinal immune system toward the commensal bacteria of our gastrointestinal tract, thus favoring the mechanisms of oral tolerance. Therefore the ST peptide could be used in immunotherapy, especially in the context of certain autoimmune diseases and certain inflammatory diseases.

An antifatigue composition by which muscular fatigue or the like during or after exercise can be reduced, is obtained by incorporating an imidazole compound, which may be obtained from extracts of fish, shellfish, fowl, flesh or the like, as an active ingredient. The imidazole compound is preferably at least one selected from the group consisting of histidine, anserine, carnosine and valenine, and salts thereof. It is further preferred to contain D-ribose as an active ingredient in the composition. By orally ingesting the antifatigue composition of the present invention, the amount of lactic acid in plasma during or after exercise can be kept low, and therefore the fatigue of muscles by exercise can be controlled. As a result, effects for improving the ability of exercise can be expected.

It is intended to provide a process for producing a highly safe seasoning by highly decomposing meat or a bone residue remaining after taking meat (in particular, chicken meat, chicken bone or culled chicken) with enzymes. It is also intended to utilize nitrogen at an elevated ratio compared with the existing low nitrogen utilization level. In decomposing protein in chicken meat, culled chicken or chicken bone, muscular protein alone is decomposed by using an autolyzing enzyme or an autolyzing enzyme with an enzymatic agent containing peptidase. In the case of using chicken meat as the raw material, it contains trace constituents of meat (taurin, anserine, carnosine, creatine, etc.) and the content of Hyp (oxyproline) is controlled to 0.5% by mass or less to the protein. In the case of using culled chicken or chicken bone as the raw material, it contains trace constituents of meat (taurin, anserine, carnosine, creatine, etc.) and the content of Hyp (oxyproline) is controlled to 1.0% by mass or less to the protein.

The invention provides feruloyl esterase and a preparing method and application thereof. A feruloyl esterase gene coming from a soil macro gene library have the nucleotide sequence and amino acid sequence shown in SEQ ID NO.1 and SEQ ID NO.2. The gene contains a tetrapeptide SXXK sequence motif which is rarely seen, and after the esterase gene is inserted into plasmid pET28a(+), the gene is transformed into escherichia coli BL21(DE3) to achieve heterogeneous expression. The molecular weight of purified recombinase (DLFae4) is 38.3 kDa. Besides, it is put forward for the first time that novel feruloyl esterase can hydrolyze penbritin, penicillin, cefazolin and other lactam antibiotics. As is shown by site-directed mutagenesis experiments, a catalysis triplet of DLFae4 is composed of serine(S11), histidine (H74) and aspartic acid (D302), and the mutation of any of serine (S11), histidine (H74) and aspartic acid (D302) can cause loss of the catalysis capability of DLFae4. DLFae4 has a high hydrolytic activity on methyl ferulate and has good heat stability. In the presence of cellulase, DLFae4 can obviously increase the amount of ferulic acid released from destarched wheat bran. Due to peculiar activities and enzymatic characteristics of novel feruloyl esterase, novel feruloyl esterase can be applied to feed, paper making, food, pharmacy and other fields.

An antifatigue composition for reducing muscular fatigue or the like during or after exercise, which includes an imidazole compound obtained from extracts of fishes, shelifishes, fowl, flesh or the like, as an active ingredient. The imidazole compound in the antifatigue composition is preferably at least one compound selected from the group consisting of histidine, anserine, carnosine, valenine and salts thereof. The antifatigue composition preferably further contains D-ribose as an active ingredient. By orally ingesting the antifatigue composition, the amount of lactic acid in plasma during or after exercise can be kept low, and therefore muscle fatigue caused by exercise can be controlled, and as a result, the ability to exercise can be improved.

The invention provides a method for extracting radiation resistant potentilla anserine polysaccharide oxide by ultrasonic waves, which comprises the following steps: extracting potentilla anserine as raw material for 30 minutes-120 minutes by ultrasonic waves and filtering under the pressure condition of 0.06 MPa-0.08 Mpa; carrying out decompressing concentration, alcohol precipitation and centrifugation on filter liquor at the pressure of 0.06 Mpa-0.08 Mpa and the temperature of 55 DEG C-65 DEG C; collecting sediments at the lower layer; freezing and drying the sediments; after dissolving by nematolyt, extracting by mixture liquid of n-butyl alcohol and chloroform, decompressing, concentrating and dialyzing, obtaining natural potentilla anserine polysaccharide. The natural potentilla anserine polysaccharide has no toxic and side effect and has better repair effect on in-vivo oxidation damage caused by free radicals generated by X-ray radiation. An antioxidant ability experiment of a small mouse radiated by a 3.5Gy X-ray indicates that the SOD activity, the T-AOC vitality and the CAT vitality are respectively improved by 66.7 percent, 54.6 percent and 105.7 percent and the MDA content is reduced by 63.2 percent of an animal with radiation damage by taking the preparation for 7 days.

The invention provides an ultrasonic-assisted method for degreasing and extracting potentilla anserina polysaccharide. The method comprises: taking potentilla anserine as a raw material, adding petroleum ether, degreasing by employing different temperature for refluxing, after a solvent is volatilized, using ethanol for normal-temperature immersing and washing, adding distilled water, transferring to ultrasonic extraction equipment, performing ultrasonic extraction filtration, collecting the water extraction liquid, adding water into the filter residue and performing ultrasonic extraction for two times, merging the water extraction liquids, performing rotary evaporation condenstation, then adding a papain solution for enzymolysis, centrifuging and getting the supernatant, performing pumping filtration by using a micro pumping filtration instrument to further remove relatively fine filter residue, concentrating the collected filtrate, then using a mixed solution of n-butanol and chloroform to perform extraction for 3-5 times, collecting the upper-layer water phase, adding ethanol into the water phase, standing, centrifuging to collect precipitate, and performing freeze drying to obtain purified potentilla anserina polysaccharide.

The invention discloses monkey-bread tree moisturizing cream. The monkey-bread tree moistening cream comprises the following ingredients in parts by weight: 3-7 parts of ANANSODNIA DIGITATA seed oil, 2-6 parts of diacetyl tartaric acid ester of mono-and diglycerides, 10-20 parts of lanolin, 0.1-0.5 parts of L-serine, 8-12 parts of a moisturizing agent, and 50-60 parts of water. The monkey-bread tree moisturizing cream is natural and mild, nearly has no irritation, can be used for nourishing the skin and moisturizing for a long time, has better water supplementing and moisturizing effects on dry skin, sensitive skin and the like, and can be used for effectively improving the skin color and increasing the skin resiliency.

The present invention relates to method of treating a patient with a condition involving increased vascular permeability or increased angiogenesis comprising administering to the patient a therapeutically effective amount of PEDF, PEDF 44 AA peptide, a homolog of the PEDF 44 AA peptide, a homolog of the PEDF 44 AA peptide wherein amino acid residues glutamate the (101) amino acid position, isoleucine at the (103) amino acid position, leucine at the (112) and serine at the (115) amino acid position are unchanged, or an agent that activates the PEDF receptor. Conditions for treatment include, but are not limited to, sepsis acute respiratory distress syndrome, nephrotic syndrome, diabetic neuropathy, preproliferative diabetic retinopathy, cancer or proliferative diabetic retinopathy.

A taste improving agent for food and beverage containing potassium chloride that can effectively relieve unpleasant tastes, such as bitter taste and harsh taste, attributed to potassium chloride without detriment to the flavor of the food and beverage; a process for producing a food and beverage containing potassium chloride of high palatability having the bitter taste and harsh taste of potassium chloride suppressed; and a food and beverage containing potassium chloride produced by the production process. Use is made of a taste improving agent comprising a basic amino acid and / or a basic peptide as an active ingredient. It is preferred that the basic amino acid be at least one member selected from among histidine, arginine and lysine and the basic peptide at least one member selected from among anserine, carnosine and balenine. This taste improving agent is added to a food and beverage containing potassium chloride, thereby obtaining a food and beverage containing potassium chloride with improved taste.

The invention provides a strategy combining metabolic engineering reconstruction with a fermentation process. On the one hand, a metabolic flow is changed to flow from a glycolysis route to an L-serine synthetic route by virtue of a metabolic engineering means, so that the sugar-acid conversion efficiency of L-serine is improved; and on the other hand, nutritional ingredients are added to meet requirements of corynebacterium glutamicum on nutrient substances after metabolic reconstruction, so that the problem of slow growth of thalli is solved, and the production strength of L-serine is improved.

Compositae cyclopeptide compounds are monocyclic homogeneous pentapeptide compounds which contain beta-phenylalanine in skeleton and are formed by the directly connected normal peptide bonds of amino acids; and generally, one compositae cyclopeptide molecule contains one L-beta-phenylalanine molecule, one L-serine molecule, one L-proline derivative molecule and two other amino acid derivative molecules. The composite cyclopeptide shown in the structural formula AstinC (1) is a new immunosuppressive agent with plant source. The invention provides a preparation method of the compounds and an application of the compounds in the preparation of the immunosuppressive medicine. In the formula, R1 and R2 represent aryl or alkyl respectively; R3, R4 and R5 represent hydroxyl or halogen atom respectively; and X and Y represent unsaturated bond or saturated bond respectively.

The invention relates to the technical field of a detection method, and discloses a high performance liquid chromatography method for measuring content of carnosine and anserine in a fish head. The high performance liquid chromatography method comprises the following steps of: taking soft tissues of the fish head, after mashing the soft tissues, adding extracting solution into the mashed soft tissues as a test sample, and carrying out heating extraction to obtain crude extract; after cooling the crude extract to the room temperature and carrying out refrigerated centrifugation, taking supernate and filtering the supernate with a millipore filter membrane; measuring the content of the carnosine and the anserine in the filtrate by high performance liquid chromatography, wherein mesoporous modified Al-SBA-15 loaded with copper ions is used as a filler in a chromatographic column of high performance liquid chromatography. According to the invention, the mesoporous modified Al-SBA-15 loadedwith the copper ions is used as the filler in the chromatographic column in high performance liquid chromatography analysis; the high performance liquid chromatography method has good absorption andseparation effects on the carnosine and the anserine, is high in detection accuracy and sensitivity and simple and convenient to operate; computer measurement can be carried out only by simple preprocessing without a fussy preprocessing step; meanwhile, deviations of a peak area and retention time are very small; and the high performance liquid chromatography method has very high accuracy.

The invention discloses a vitamin B12 complex fermenting addition agent and an application method thereof to fermenting vitamin B12. The vitamin B12 complex fermenting addition agent comprises the following components in percentage by mass of: 90-98% of betaine, 0.15-0.55% of vitamin B complex and 1.45-9.85% of complex amino acid, wherein the vitamin B complex comprises the following components in mass percentage: 30-55% of thiamine, 10-25% of pyridoxine hydrochloride and 40-55% of folic acid; and the complex amino acid comprises the following components in percentage by mass of: 5-10% of lysine, 10-15% of serine, 10-15% of glutamic acid, 30-35% of asparagine and 30-40% of aspartic acid. The complex fermenting addition agent disclosed by the invention is added into the fermented vitamin B12 so that the supply of various nutrient components in a fermentation process is ensured and the fermentation output of the vitamin B12 is effectively improved.

A glycoprotein and / or a glycopeptide which are a test substance is heated in the presence of a pyrazolone derivative, an isoxazolone derivative, a hydantoin derivative, a rhodanine derivative, a maleimide derivative, or the like under a basic condition to cleave and label a post-translational modification group for analysis, thereby enabling analysis of a post-translational modification of a serine residue and / or a threonine residue.

The invention discloses a stress-resistant gene SiRLK35 of setaria italica L.Beauv. as well as encoding protein and an application. Receptor-like protein kinase SiRLK35 is separated from setaria italica L.Beauv., the nucleotide sequence of the receptor-like protein kinase SiRLK35is shown in SEQ ID NO.1, and the gene encoding protein product belongs to serine / threonine protein kinase. The expression pattern of the gene in a stress condition is researched, the prokaryotic expression vector of the gene is established, the gene can be expressed in the protein level as is verified, the functions of the gene are primarily verified with a dot assay through a gene prokaryotic expression system, meanwhile, the growth curve of the gene under a salt treatment condition is detected, the gene is heterologously transformed into rice, a corresponding transgenetic plant is obtained, the functions of the transgenetic plant in salt resistance and stress resistance are identified primarily, targeted quality and character improvement on setaria italica L.Beauv. is further performed purposefully, the new stress-resistant germplasm of setaria italica L.Beauv. is innovated, and a guidance function is performed.

The invention provides preparation methods of two kinds of anserine and a preparation method of a synthetic anserine intermediate. The preparation methods are simple to operate, easy to implement, stable in process, easy to control, convenient for reaction post-treatment, good in product yield, high in purity and capable of being economically and conveniently used for industrial production.

This invention relates to novel phosphorylation sites in cardiac Troponin I that are associated with the onset of heart failure. The phosphorylation sites, i.e., serine 5, tyrosine 26, threonine 51, serine 166, threonine 181 and / or serine 199, can be used as biomarkers for (i) identifying subjects at risk for the development of heart failure, (ii) treating subjects having a higher than normal level of the biomarker, and (iii) monitoring therapy of a subject at risk for the development of heart failure. Also described are antibodies, reagents, and kits for carrying out a method of the present invention.

L-serine, L-serine precursors, L-serine derivatives and L-serine conjugates for treatment, amelioration and / or prevention of protein aggregation / tangles / plaques and diseases associated with protein aggregation / tangles / plaques. In particular, treatments and uses for L-serine, L-serine precursors, L-serine derivatives and L-serine conjugates include Alzheimer's disease (AD), Parkinson's disease, Amyotrophic Lateral Sclerosis (ALS), and Huntington disease (HD).

The present invention provides a medicine composition for improving sleep. Said medicine composition includes the following several portions: antihistamine; one or two kinds of Chinese medicinal materials selected from uncariae uncis cum ramulus, humulus lupulus, ginseng radix, avena sativa, viscum album, glycyrrhizae radix, poria, cnidii rhizome, anemarrhenae rhizome, arisaematis tuber, moutan cortex, potentilla anserine, camomile, piper methysticum and tilia spp.; perfume component; compound selected from vitamin and / or mineral substance; and any one component selected from melatonin compounds.

The invention relates to a composition and application thereof in reduction of uric acid and treatment or prevention of gout. The composition provided by the invention is prepared from quercetin and anserine. The composition can effectively reduce the uric acid and treat hyperuricemia and the gout.

A soybean-yak marrow milk is proportionally prepared from purified water, soybean, yak narrow, almond, oats, walnut kernel, wolfberry fruit, potentilla anserine and highland barley through respective pulverizing, proportional mixing, high-temp processing to become milk, and cooling. It is an ideal health-care food for supplementing Ca, improving immunity, and preventing and treating rachitis, cardiovascular and cerebrovascular diseases, rheumatism, and dermatopathy.

The invention relates to weight losing soft capsules. The weight losing soft capsules are composed of soft capsule bodies and content in the capsule bodies. The content is prepared from, by weight, 20-33% of Tibet potentill anserine, 15-25% of lotus leaf extract, 15-25% of apple extract, 15-25% of spinach extract, 15-25% of coix seed powder, 5-15% of chitosan, 1-10% of blueberry extract and 1-10% of honeysuckle flower extract; the soft capsule bodies are prepared from 30-40% of soybean polysaccharide, 45-54% of pullulan, 2-10% of humectant and 4-8% of gel. The weight ratio of the soft capsule bodies and the content is 1:(2-4). The soft capsules are simple and reasonable in raw material composition, appropriate in matching, convenient to take and good in weight losing effect and have natural components and a healthcare function. The preparing method is reasonable in process and high in operability, and large-scale production is easy to achieve.

The invention discloses fish polypeptide dry powder containing anserine, a uric-acid-reducing peptide composition, a uric-acid-reducing peptide beverage and preparation methods. A preparation method of the fish polypeptide dry powder containing anserine comprises the following steps of mincing tuna meat, performing rinsing with rinsing liquid, and performing spray drying to obtain the fish polypeptide dry powder. According to the preparation method of the fish polypeptide dry powder containing the anserine, tunas are used as raw materials for preparing the fish polypeptide dry powder, the technology is simple, the cost is low, and the effect is stable. The uric-acid-reducing peptide composition prepared from the fish polypeptide dry powder is cooperative in components and reasonable in compatibility of components, and the uric-acid-reducing peptide composition which is safe, efficient and free from toxic sides and has notable effect of reducing uric acid is prepared.