7 results about "Xanthine oxidase activity" patented technology

The present application belongs to the technical field of bioactive peptides, functional food and biological medicine, and particularly relates to a marine fish-derived xanthine oxidase inhibiting heptapeptide NPLVTPP and its application in reducing uric acid. The present application discloses a marine fish-derived heptapeptide NPLVTPP with xanthine oxidase inhibiting activity, and the amino acid sequence of the heptapeptide is Asn-Pro-Leu-Val-Thr-Pro-Pro. The heptapeptide can be prepared by solid-phase chemical synthesis or marine fish protein enzymatic separation and purification, can significantly inhibit xanthine oxidase activity, reduce uric acid generation, can effectively reduce serum uric acid level in a hyperuricemia animal model, and can improve high uric acid related kidney function damage. The present application also protects the application of the heptapeptide in preparing xanthine oxidase inhibitors, uric acid reducing products, products for preventing or improving hyperuricemia and kidney damage, and a medicine, food or nutritional composition containing the heptapeptide. The polypeptide sequence of the present application is clear, the target is clear, the in vivo and in vitro efficacy is clear, the source is safe, and the polypeptide can be used in the fields of functional food and biological medicine.

The application discloses a bird's nest peptide for inhibiting xanthine oxidase and application thereof, and belongs to the technical field of functional polypeptides. The amino acid sequence of the bird's nest peptide for inhibiting xanthine oxidase is WGLL, can chelate an active center of xanthine oxidase (XOD), and is helpful for inhibiting the activity of XOD in catalyzing conversion of xanthine or hypoxanthine. The application further provides a bird's nest peptide powder containing the bird's nest peptide for inhibiting xanthine oxidase. The bird's nest peptide powder can significantly reduce the rate of conversion of xanthine or hypoxanthine into uric acid catalyzed by XOD, has good uric acid reducing effect, can be applied to development of uric acid reducing products or various uric acid reducing products, and has high market value.

The present application relates to the field of biotechnology, in particular to astaxanthin and dihydroquercetin co-loaded liposome and its application in preparation of uric acid-lowering products. According to the steps of "in vitro function screening - optimal ratio determination - co-loaded liposome construction - surface coating and stabilization treatment - uric acid-lowering application verification", the obtained system is verified through physicochemical characterization, stability test and animal experiment. The results show that the co-loaded liposome can reduce the serum uric acid level of high uric acid model mice, inhibit the activity of liver xanthine oxidase, and improve the related indexes of kidney function and histopathological damage.

The application discloses a composition with a function of reducing uric acid and a preparation process thereof, which is prepared from the following raw materials in parts by weight: 10-30 parts of chicory, 5-20 parts of gardenia, 5-15 parts of mulberry leaves, 5-15 parts of radix puerariae, 10-20 parts of coix seed, 5-15 parts of poria cocos, and 2-8 parts of licorice root. The application has the beneficial effects that: the seven kinds of medicine and food homologous medicinal materials such as chicory and gardenia are scientifically matched, uric acid is reduced through the double ways of inhibiting xanthine oxidase activity and promoting uric acid excretion, the animal experiment shows that the effect is better than that of the positive drug allopurinol, and the safety is high, and there is no toxic side effect; the preparation process is simple, there is no organic solvent residue, various oral dosage forms can be prepared, and the application is suitable for the auxiliary conditioning of hyperuricemia and gout.

This invention belongs to the field of traditional Chinese medicine technology, and provides a compound traditional Chinese medicine extract for lowering uric acid, its preparation, and application. Using hawthorn, astragalus, poria cocos, jujube, and tangerine peel as raw materials, this invention obtains ethanol extract S1 and water extract S2 through ethanol extraction and water extraction. Both S1 and S2 have antioxidant activity, targeting DPPH and ABTS. + The free radicals exhibit significant scavenging capabilities and can effectively inhibit xanthine oxidase activity. Furthermore, S1 and S2 can reduce serum and liver XOD activity in hyperuricemic mice, improve liver and kidney function indicators such as AST, ALT, TP, UREA, SCr, and CysC, and decrease serum uric acid levels in a dose-dependent manner; high doses can lower uric acid levels below normal. The extract of this invention possesses uric acid-lowering, antioxidant, and liver and kidney protective effects, showing good therapeutic effects on hyperuricemia and good protective and preventive effects against liver and kidney damage caused by hyperuricemia.

The invention relates to the technical field of biological fermentation, in particular to lactobacillus rhamnosus and application thereof. The preparation method of the fermented radix puerariae extract comprises the following steps: adding water into the radix puerariae extract, carrying out high-temperature sterilization, adding lactobacillus rhamnosus LK-A28 according to an inoculum size of 3%, and carrying out inoculated fermentation, so as to enhance the inhibition effect of the radix puerariae extract on xanthine oxidase. The fermented kudzuvine root extract can regulate hyperuricemia caused by purine metabolism disorder, oxidation of hypoxanthine and xanthine into uric acid is reduced by inhibiting xanthine oxidase XOD, the fermentation condition of the fermented kudzuvine root extract is simple, the xanthine oxidase activity inhibiting capacity is remarkably improved after fermentation, industrial production is easy, and the fermented kudzuvine root extract is suitable for industrial production. Good development and application prospects are realized in treatment of hyperuricemia.

The application discloses a kind of smilax glabra inhibiting xanthine oxidase active ingredient extraction process and its extraction device. By once reflux extraction, the fine powder of smilax glabra is placed in the material supporting filter frame, ethanol solution is placed in the extraction tank, so that the fine powder of smilax glabra and ethanol solution are separated by the material supporting filter frame, then the obtained extraction liquid after extraction is extracted into the solvent turnover box, then the solvent in the solvent turnover box is extracted into the extraction tank to repeat the step S3 to circulate reflux extraction 3-4 times, so that the extraction liquid can be separated from the fine powder of smilax glabra without pouring out for filtration. Compared with the traditional pouring-out filtration method, the extraction efficiency is improved, the operation is simple, the heat release and heat absorption of the heating liquid are fully utilized during the whole process, thereby realizing resource recycling, achieving the purpose of energy saving, and improving economic benefits.