Cucumber carotenoid phytoene synthetase gene

A technology of phytoene and carotene, applied in the field of genetic engineering

Inactive Publication Date: 2012-11-14
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the cloning of the full-length gene of cucumber carotenoid synthase at home and abroad

Method used

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  • Cucumber carotenoid phytoene synthetase gene
  • Cucumber carotenoid phytoene synthetase gene
  • Cucumber carotenoid phytoene synthetase gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A cucumber carotenoid phytoene synthase gene, the length of its nucleotide sequence SEQ ID is 1479bp, has the following nucleotide sequence:

[0020] AGTGGGAGTTTCATCCGTGTCGAAGCATTGCTTAGAATTTCTTGGGCGGTTTAGTTGGATTTAAGTTATCTTTGCTTTTCCATTTCATTTTGCTACTGGGTTTCTCGATTCCTCCTGCGATTCGTTCATTGAAAGCCTTGGAATTCTTCTAGTGGGATTAAAATCGATTGATATGTCTTTAGCTTCATCGTTAGTTGTTTCTCCCAACGTTGAACTTTCCCCATCCAACTTTGGGTTTCTTGATTCTCTTCGAGATGGACCCCAAATTCCCGATTCTTTTAGATTCTTTTCGCGAAATCGAGTGCCAAATCTGATTAACAAGAGACAGAAGTGGGGGAATCCCTCTCATTCTACAGAACTGAAATACCCAATTCTCTGTGAAAGTGGATATGGGTCTGTTATTGCAGCAAGTATGGTGGCGAATCCCGCCGGAGAAATGGCCGTCTCAGCTGAGCAGAAGGTGTATAACGTAGCTATGAAACAGGCGGCTCTGGTGAAACGACAACTTAGAACCGCCGGAGAATTGGATGTGAAGCCGGATATTGTTCTTCCGGGGACTTTGAGCTTGTTGAATGAGGCTTATGATCGTTGTGGTGAAGTTTGTGCAGAGTATGCCAAGACATTTTATCTGGGAACTATGTTGATGACACCGGAGAGGCAAAAGGCTATTTGGGCAATTTATGTATGGTGTAGGCGGACAGATGAACTTGTTGATGGGCCAAATGCTTCACACATAACACCTACTGCATTGGACAGATGGGAGGCAAGGTTGGAAGAGCTTTTCCAAGGGAGGCCATTCGATATGCTCGATGCAGCTTTGGCGGATACCGTTACTAA...

Embodiment 2

[0022] A polypeptide encoded by the gene of claim 1, comprising 422 amino acids, a molecular weight of 47393.507Da, and an isoelectric point of 6.545. At pH 7.0, the charge is 0.777. 具有以下的氨基酸序列:MSLASSLVVSPNVELSPSNFGFLDSLRDGPQIPDSFRFFSRNRVPNLINKRQKWGNPSHSTELKYPILCESGYGSVIAASMVANPAGEMAVSAEQKVYNVAMKQAALVKRQLRTAGELDVKPDIVLPGTLSLLNEAYDRCGEVCAEYAKTFYLGTMLMTPERQKAIWAIYVWCRRTDELVDGPNASHITPTALDRWEARLEELFQGRPFDMLDAALADTVTKFPVDIQPFKDTIEGMRMDLRKSRYKNFDELYLYCYYVAGTVGLMSVPVMGIAPESEASTESVYNAALALGIANQAPPNILRDVGEDARRGRIYLPQDELAQAGLSDEDIFAGRVTDKWRNFMKSQIKRARMFFDEAEKGVLELNKASRWPVWASLLLYRQILDGIEANDYNNFTKRAYVRKGKKILALPMAYARALLGPS*。

Embodiment 3

[0024] A recombinant plasmid pT-PSY comprising the gene of claim 1, its length is 4515bp, comprising an ampicillin selection marker; it has the following nucleotide sequence:

[0025]

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PUM

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Abstract

The invention discloses a cucumber carotenoid phytoene synthetase gene. All cucumber carotenoids are synthesized by isoprenoid compounds or terpenoids. The length of the nucleotide sequence SEQ ID of the cucumber carotenoid phytoene synthetase gene is 1479bp, and the cucumber carotenoid phytoene synthetase gene has a specific nucleotide sequence. The gene-coded polypeptide of claim 1 comprises 422 amino acids, has the molecular weight of 47393.507Da, the isoelectric point of 6.545, the charge of 0.777 when the pH value is 7.0, and has a specific amino acid sequence. The recombinant plasmid pT-PSY of the gene of claim 1 has the length of 4515bp, comprises ampicillin selection markers, and has a specific nucleotide sequence. The invention is used for cloning cucumber carotenoid phytoene synthetase genes.

Description

Technical field: [0001] The invention relates to the technical field of genetic engineering; in particular, it relates to a gene for cloning cucumber carotenoid phytoene synthase. Background technique: [0002] All cucumber carotenoids are synthesized via the isoprenoid or terpenoid pathway. There are 5 enzymes involved in its metabolic main chain, namely phytoene synthase phytoene synthase gene PSY, phytoene desaturase PDS, ξ-carotene desaturase ZDS, tomato Lycopene β-cyclase LycB and lycopene ε-cyclase LycE. With the development of molecular biology research methods, genes encoding these enzymes have been isolated and identified from tomato, tobacco, pepper, Arabidopsis, corn and other plants. At present, the cloning of the full-length gene of cucumber carotenoid synthase has not been reported at home and abroad. Invention content: [0003] The purpose of the invention is to provide a design sequence of the known phytoene synthase gene PSY of other plants as a primer,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/52C12N9/00C12N15/63
Inventor 秦智伟劳杉杉
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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