A kind of chromosome production method

A chromosome production and chromosome technology, applied in the fields of genetics and chromosome analysis, can solve the problems of inability to form a water film, rarely obtaining fully dispersed chromosomes, and high surface tension of glass slides.
CN103308361BActive Publication Date: 2015-09-30JILIN TUO HUA BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JILIN TUO HUA BIOTECH
Publication Date
2015-09-30

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Abstract

The invention relates to a chromosome slide preparing method which comprises the following steps of: providing a chromosome sample, putting a slide into water and keeping for more than 2 hours at 2-8 DEG C, carrying out pretreatment on the slide, fixing the chromosome sample, and dripping on the slide. The chromosome obtained by using the improved chromosome slide preparing method is sufficient in dispersion, free of overlapping, free of loss, clear in strip and regular in morphology; and the analysis on chromosome karyotype and the diagnosis on diseases are facilitated.
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Description

technical field

[0001] The invention belongs to the technical field of genetics. Specifically, the present invention relates to the technical field of chromosome analysis; more specifically, it relates to a method for chromosome preparation. Background technique

[0002] Chromosomes are the genetic material in the nucleus of a cell. After the lymphocytes in the blood are cultured under certain conditions, they are harvested, made into slices, digested with trypsin, and stained to show the bands. Chromosomes can be observed under a microscope. Humans have 23 pairs of chromosomes, of which 22 are autosomes and 1 is the sex chromosome that determines sex. Genes on chromosomes that carry genetic information. An increase or decrease in the number of chromosomes, or structural abnormalities such as deletions and translocations of chromosomes are called chromosomal diseases, and corresponding clinical symptoms may appear. Whether the number and structure of chromosomes are norm...

Claims

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