The present invention discloses a novel antibacterial textile fabric preparation method, and belongs to the field of textile processing, wherein nanometer silver particles and nanometer titanium dioxide particles are respectively sputtered on a fabric substrate through a magnetron sputtering technology, and the formed uniform nanometer composite film is covered on fibers so as to obtain the antibacterial fabric. The preparation process comprises: 1, carrying out a fabric pretreatment, wherein washing and drying are performed; and 2 sputtering nanoparticles, wherein the pretreated fabric substrate is placed onto a sample holder in the sputtering chamber, the distance between the target and the fabric substrate is 40-100 mm, a water cooling device is adopted to cool the substrate, the reaction chamber is evacuated to achieve the background vacuum, and high-purity argon gas with a volume fraction of 99.999% is introduced as the sputtering gas. According to the present invention, the method has characteristics of simple processing, easy control, low cost, high efficiency, energy saving, no pollution, and easy industrial production achieving; and the nanometer silver and the nanometer TiO2 are combined to prepare the antibacterial textile fabric so as to substantially improve the antibacterial property of the material and provide the new idea and new method for the preparation of the antibacterial fabric.