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Antibacterial nanocomposite film material of graphene oxide and lysozyme, preparation method and application of antibacterial nanocomposite film material as antibacterial material

A nano-composite and thin-film material technology, applied in botany equipment and methods, applications, nanotechnology, etc., can solve the problems of low mechanical strength, poor stability, and difficulty in forming a continuous thin-film structure, and achieve a highly ordered structure Effect

Inactive Publication Date: 2011-08-31
NANJING UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

Due to its strong bactericidal ability, lysozyme has a wide range of application values. Of course, as a water-soluble protein molecule, it has the general disadvantages of protein molecules, such as low mechanical strength, and it is difficult to form a continuous film structure on a solid surface. Poor stability, will dissolve rapidly in aqueous solution, etc.

Method used

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  • Antibacterial nanocomposite film material of graphene oxide and lysozyme, preparation method and application of antibacterial nanocomposite film material as antibacterial material
  • Antibacterial nanocomposite film material of graphene oxide and lysozyme, preparation method and application of antibacterial nanocomposite film material as antibacterial material
  • Antibacterial nanocomposite film material of graphene oxide and lysozyme, preparation method and application of antibacterial nanocomposite film material as antibacterial material

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Embodiment Construction

[0043] 1. Preparation of graphene oxide / lysozyme antibacterial nanocomposite film materials

[0044] 1.1 Synthesis of graphene oxide.

[0045] 1.1.1 Oxidation of graphite

[0046] The oxidation of graphite uses a modified Hummers method. The graphite used as the raw material is 325 mesh powder with a purity of 99.9995%, which was purchased from Alfa Aesar Company, and chemical reagents such as potassium permanganate, sodium nitrate, concentrated sulfuric acid, hydrochloric acid, and hydrogen peroxide were purchased from Nanjing Chemical Reagent Company.

[0047] Take 2g of graphite powder, mix it with 3.75g of sodium nitrate and place it in a large beaker; install an electric stirrer to keep the stirrer working continuously throughout the subsequent oxidation process. Slowly pour 170 mL of concentrated sulfuric acid, and continue stirring for 2 hours to mix the reagents evenly. The beaker was placed in an ice bath, potassium permanganate was slowly added in batches, a total...

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Abstract

The invention relates to an antibacterial nanocomposite film material of graphene oxide and lysozyme, a preparation method and application of the antibacterial nanocomposite film material as an antibacterial material. In the preparation method of the antibacterial nanocomposite film material of graphene oxide and lysozyme, with a mixed solution of the lysozyme and the graphene oxide as a raw material, the antibacterial nanocomposite film material of graphene oxide and lysozyme is prepared by using a solution casting method, wherein a mass ratio of the lysozyme to the graphene oxide in the mixed solution of the lysozyme and the graphene oxide is (2-15):1. By using the preparation method, the antibacterial nanocomposite film material of graphene oxide and lysozyme can be obtained. The antibacterial nanocomposite film material of graphene oxide and lysozyme is applied as the antibacterial material. The composite film can be spread on surfaces of other solids to have an antibacterial protection effect. In the invention, a novel functional nanocomposite film material on the basis of a graphene oxide nano-plate and a lysozyme protein, which has a highly ordered structure, a controlled-release character and an antibacterial function, is designed and prepared.

Description

technical field [0001] The invention relates to a graphene oxide / lysozyme antibacterial nanocomposite film material, a preparation method thereof and an application as an antibacterial material. Background technique [0002] Composite materials are widely used in aerospace, defense, medical, transportation, sports and other fields due to their excellent comprehensive properties, especially the designability of their properties; organic / inorganic nanocomposites are the most attractive part of them. It can combine some excellent properties of organic and inorganic materials (such as the versatility of organic materials and the excellent physical properties of inorganic materials), effectively adjust the structure at the nanoscale, and then obtain more excellent performance and broad Therefore, it has received extensive attention in recent years. [0003] Prepare organic / inorganic multilayer composite film materials at the molecular level, assemble different types and function...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00B82Y40/00
Inventor 马余强元冰
Owner NANJING UNIV
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