Antibacterial nanocomposite film material of graphene oxide and lysozyme, preparation method and application of antibacterial nanocomposite film material as antibacterial material
A nano-composite and thin-film material technology, applied in botany equipment and methods, applications, nanotechnology, etc., can solve the problems of low mechanical strength, poor stability, and difficulty in forming a continuous thin-film structure, and achieve a highly ordered structure Effect
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[0043] 1. Preparation of graphene oxide / lysozyme antibacterial nanocomposite film materials
[0044] 1.1 Synthesis of graphene oxide.
[0045] 1.1.1 Oxidation of graphite
[0046] The oxidation of graphite uses a modified Hummers method. The graphite used as the raw material is 325 mesh powder with a purity of 99.9995%, which was purchased from Alfa Aesar Company, and chemical reagents such as potassium permanganate, sodium nitrate, concentrated sulfuric acid, hydrochloric acid, and hydrogen peroxide were purchased from Nanjing Chemical Reagent Company.
[0047] Take 2g of graphite powder, mix it with 3.75g of sodium nitrate and place it in a large beaker; install an electric stirrer to keep the stirrer working continuously throughout the subsequent oxidation process. Slowly pour 170 mL of concentrated sulfuric acid, and continue stirring for 2 hours to mix the reagents evenly. The beaker was placed in an ice bath, potassium permanganate was slowly added in batches, a total...
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