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A method for the determination of 9-hydroxyfluorene based on an electrochemical hairpin DNA biosensor

An electrode and gold electrode technology, applied in the field of electrochemical detection and chemical sensor, can solve the problems of less detection, poor uniformity and stability of DNA membrane, non-selectivity of phenolic compounds, etc., and achieves low cost and simple method. , environmental disturbances and less restrictive effects

Inactive Publication Date: 2016-04-06
BEIJING NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Combined with nanotechnology, Zheng et al. modified natural herring sperm DNA to the surface of glassy carbon electrode (GCE) modified by multi-walled carbon nanotubes (MCNTs) to prepare a DNA / MWNTs / GCE sensor, which realized the detection of phenol, methylphenol, and catechin. For the determination of phenolic pollutants such as phenol, the detection limit reaches the order of micromolar. The disadvantage of this sensor is that it is not selective for phenolic compounds.
[0004] At present, the use of electrochemical DNA biosensors to detect hydroxyaromatic compounds is still relatively small, and the natural DNA sequences are mainly used. Although these DNA sensors have high sensitivity, they are not selective for phenolic compounds, and through The DNA membrane modified by adsorption to the electrode surface has poor uniformity and stability
So far, the application of artificially synthesized sulfur-modified DNA sensors for the detection of hydroxyphenolic compounds has not been reported. The sulfur-modified DNA self-assembles on the surface of the gold electrode through the interaction of gold-sulfur bonds, and the DNA membrane structure is highly ordered. , good stability, and the artificially synthesized DNA can adjust the base, and the design of a DNA biosensor with high specificity and sensitivity is conducive to the realization of selective analysis and detection of the target in the mixed system, thus developing a A DNA sensing and detection technology with special selectivity for a certain hydroxyl compound is of great significance in improving the existing detection technology in my country

Method used

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  • A method for the determination of 9-hydroxyfluorene based on an electrochemical hairpin DNA biosensor
  • A method for the determination of 9-hydroxyfluorene based on an electrochemical hairpin DNA biosensor
  • A method for the determination of 9-hydroxyfluorene based on an electrochemical hairpin DNA biosensor

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Experimental program
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Effect test

Embodiment 1

[0032] 1) Put the gold electrode on the buckskin polishing cloth with 0.05μm α-Al 2 o 3 The powder is polished to a mirror surface. After polishing, the surface dirt is first washed away, and then ultrasonically cleaned in ethanol and deionized water, each time for 2 to 3 minutes, and repeated three times; then in 1mol / LH 2 SO 4The solution was scanned by cyclic voltammetry until it was stable, and finally rinsed with deionized water and dried with nitrogen to obtain a gold electrode with a clean surface;

[0033] 2) High ionic strength Tris-NaClO with pH=7.4 4 Buffer solution to dissolve solid hairpinDNA, in which Tris concentration is 20mM, NaClO 4 The concentration is 300mM, mixed with a vortex shaker, and left at room temperature for 12 hours, under which conditions the hairpinDNA structure is formed;

[0034] 3) Soak the activated gold electrode in the pre-prepared 5 μM hairpinDNA solution, and let it stand at room temperature for 4-5 days to obtain the hairpinDNA mod...

Embodiment 2

[0039] The 9-hydroxyfluorene solution in Example 1 was replaced by 275nmol / L 2-hydroxyfluorene solution, and other conditions were the same as in Example 1. Experiments showed that the hairpinDNA modified electrode had no response to 2-hydroxyfluorene.

[0040]

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Abstract

The invention belongs to the technical field of electrochemistry detection and chemical sensors for detecting 9-hydroxy fluorine based on a hairpin DNA modified gold electrode sensor, and relates to preparation of a hairpin DNA modified gold electrode capble of having electrochemical response to 9-hydroxy fluorine and detection on the 9-hydroxy fluorine with the electrode. Specifically, the synthetic sulfhydryl modified hairpin DNA is used as a raw material, the hairpin DNA is self-assembled to the surface of the gold electrode by the Au-S bond interaction to prepare the DNA modified electrode. The immobilized DNA electrode has high response to the 9-hydroxy fluorine, and the detection on the 9-hydroxy fluorine can be carried out according to the change of response of the electrochemistry to the impedance. The DNA sensor has the advantages of mild preparation condition, simplicity in operation, good stability and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of electrochemical detection and chemical sensors, and relates to the preparation of a hairpinDNA-based biosensor and the use of the prepared sensor for detecting 9-hydroxyfluorene. Background technique [0002] As derivatives of aromatic hydrocarbons, hydroxyaromatic hydrocarbons have attracted much attention due to their strong biological toxicity and environmental hazards. Some aromatic phenolic pollutants have been included in the priority monitoring blacklist. Due to its wide range of uses, it is contained in the wastewater discharged from many industrial fields such as gas, coking, oil refining, metallurgy, machinery manufacturing, glass, petrochemical, wood fiber, chemical organic synthesis industry, plastics, medicine, pesticide, paint and other industries. Hydroxyphenol, so it is difficult to prevent its pollution. If it is discharged directly, it can pollute the air, water, soil and food, so its mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/26
Inventor 刘新会梁刚李晓宏巩文雯刘冠男陶莉
Owner BEIJING NORMAL UNIVERSITY
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