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Production method of triploid yellow catfish

A production method and technology for yellow catfish are applied in the production field of triploid yellow catfish, can solve the problems of low survival rate, slow growth rate of yellow catfish, etc., achieve strong disease resistance, improve induction rate and survival rate , the effect of large individual size

Inactive Publication Date: 2008-11-19
SHAOXING UNIVERSITY +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of slow growth rate and low survival rate of yellow catfish in the prior art, the object of the present invention is to provide a production method of triploid yellow catfish, the yellow catfish obtained by the production method has a fast growth rate. , high survival rate characteristics

Method used

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  • Production method of triploid yellow catfish
  • Production method of triploid yellow catfish

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Embodiment Construction

[0019] refer to figure 1 Shown, the production method of triploid yellow catfish of the present invention is as follows:

[0020] 1.1 Material preparation

[0021] 1.1.1 Sample

[0022] Yellow catfish (Pelteobagrus fulvidraco) was obtained from the Shaoxing farmer's market. Visually, the gonads were well developed.

[0023] 1.1.2 Reagents

[0024] Inducing reagent: 6-Dimethylaminopurine (6-Dimethylaminopurine, referred to as 6-DMAP), a product of Sigma Company in the United States. Molecular formula C 7 h 9 N 5 , molecular weight 163.2, drug purity higher than 98%.

[0025] Genome mini-extraction kit (including Cell Lysis Solution; PrecipitationSolution; TE (pH=8.0); 1.2mol / L NaCl; Proteinase K; Rnase A) is a product of Bioengineering (Shanghai) Co., Ltd.

[0026] 1.1.3 Instruments

[0027] Several water tanks, air pump, 1 set of dissecting apparatus, 1.5ml digestive tube, pipette gun, electronic analytical balance (JA5003, used for weighing trace reagents, the maxim...

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Abstract

The invention discloses a production method of triploid pseudobagrus fulvidraco, which belongs to the fish culture technology field. Artificial insemination is performed to the pseudobagrus fulvidraco female parent and male parent which are obtained through artificial induced spawning, after 10 to 15 minutes, triploid pseudobagrus fulvidraco is obtained through induction, and the duration time for induction is 10 to 20 minutes. After the triploid pseudobagrus fulvidraco is obtained through induction, the multiple of the chromosome is measured through the technologies, such as the karyotype analysis of chromosome, the flow cytometer, etc., and the triploid pseudobagrus fulvidraco is identified and sieved for culture. The method restrains the releasing time of the second polar body through time control on the artificial insemination, the induction through the physical or chemical method, and control on the induction duration time, and thereby the inductivity and the survival rate of the triploid pseudobagrus fulvidraco are greatly increased, and according to the experiment statistics, the artificial inductivity of the achieved triploid pseudobagrus fulvidraco is more than 50 percent, and the survival rate is more than 80 percent.

Description

technical field [0001] The invention relates to a method for cultivating fish, in particular to a method for producing triploid yellow catfish. Background technique [0002] Yellow catfish [Pelteobagrus fulvidraco (Richardson)] is commonly known as yellow curd, yellow drum fish, etc., and belongs to Siluriformes, Paliidae, and Pelteobagrus genus. Its meat is tender and tender, without intermuscular spines, with a protein content of 15.37%, a total amino acid content of 14.19%, and an essential amino acid index of 73.34, which is higher than other fish except mandarin fish. Has high nutritional value. At the same time, because of its sweet and flat meat, and the effect of expelling wind and diuresis, it can be used to treat edema, sore throat and other diseases, and has certain medicinal value, so it is favored by consumers. At present, due to the sharp reduction of natural resources of yellow catfish, the price of yellow catfish on the market has been rising year by year (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 罗文沈文英邓先余
Owner SHAOXING UNIVERSITY
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