Human peripheral blood chromosome synchronization preparation kit

Abstract

The invention discloses a human peripheral blood chromosome synchronization preparation kit which comprises thymidine, 2'-deoxycytidine, colchicine, a 1640 culture medium and a fixing solution. According to the kit, growth cycles of peripheral blood lymphocytes are synchronized, enough 500-550 chromosome karyotypes with stripes can be obtained, so that chromosomal aberration and small abnormal structures are accurately identified. The synchronization kit adopting excessive thymidine single occlusion, deoxycytidine releasing and ethanol acetic acid harvesting is capable of increasing resolution ratio, simple in operation steps, capable of reducing the contact frequency of toxic reagents, and suitable for wide and conventional application.

Description

technical field [0001] The invention belongs to the field of medical equipment, and in particular relates to a human peripheral blood chromosome synchronization preparation kit. Background technique [0002] Chromosomes are the basic substance that makes up the nucleus and the carrier of genes. Diseases caused by abnormal chromosome numbers and / or structural aberrations are called chromosomal diseases, which constitute a large category of human genetic diseases. The number of human chromosomes that have been discovered so far There are more than 20,000 abnormalities and structural aberrations, and more than 200 chromosomal syndromes, which are one of the main causes of miscarriage, stillbirth, congenital abnormalities of development, and hermaphroditism. Cell culture and karyotype analysis after chromosome preparation are classic cytogenetic techniques for discovering chromosomal diseases, and peripheral blood is the most widely used specimen for chromosome analysis. [000...

AI Technical Summary

Problems solved by technology

High-resolution karyotype analysis technology can reach more than 800 chromosome bands, but the whole experimental process is cumbersome, requires high technical requirements for the readers, is time-consuming and laborious, and cannot be used as a routine clinical application

At present, the G-banding standard of the routine method of peripheral blood chromosome preparation in my country is 320-400 bands, but this often leads to misdiagnosis and missed diagnosis of many abnormal structures

To obtain more chromosomes, it is necessary to obtain a large number of cells in the early and metaphase of division; if the technology is required to be simple and operable at the same time, it is difficult to achieve in the conventional method of ordinary culture

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