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Method and kit for detecting chromosome abnormality before embryo implantation

A chromosomal abnormality and preimplantation technology, which is applied in the field of preimplantation chromosomal abnormality detection, can solve the problems of large fluctuation of sequencing data, low accuracy, affecting the success rate of IVF implantation and birth rate, etc., so as to improve the results. Accuracy, the effect of improving accuracy

Pending Publication Date: 2017-09-22
ANNOROAD GENE TECH BEIJING +2
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AI Technical Summary

Problems solved by technology

For example, using the traditional whole-genome amplification method combined with the traditional library construction method can detect all the states of chromosomes, but the sequencing data obtained by this method fluctuates greatly, resulting in a decrease in the accuracy of chromosome abnormality detection. This problem occurs in some regions of chromosomes. Abnormal results, such as DNA copy number variations (CNV for short), have a particularly serious impact on test results
Increase the risk of misdiagnosis, affect the implantation success rate and birth rate of IVF, and increase the birth defect rate

Method used

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  • Method and kit for detecting chromosome abnormality before embryo implantation
  • Method and kit for detecting chromosome abnormality before embryo implantation
  • Method and kit for detecting chromosome abnormality before embryo implantation

Examples

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Effect test

Embodiment 1

[0083] 1 Whole Genome Amplification

[0084] Select an embryo at the blastocyst stage that has developed for 5 to 6 days, and take 3 parts of trophoblast cells, and take 3 to 5 cells from each part, and name them as sample 1, sample 2 and sample 3 respectively. Samples 1 to 3 were treated with sterile ddH 2 O to make up to 9μL system.

[0085] Perform the following operations on sample 1.

[0086] 1.1 Cell Lysis

[0087] Configure the lysate Mix, as shown in Table 1 below,

[0088] Table 1

[0089]

[0090] Take 1 μL of the lysis solution Mix and add it to the 9 μL system in the PCR tube. Pay attention to add the lysis solution Mix to the tube wall, and keep the tip of the pipette away from the liquid at the bottom of the tube. At this time, the inner volume of the tube is 10 μL, and after centrifugation, put it on the PCR instrument. PCR cleavage program (hot lid 105°C), the program is shown in Table 2 below, and the cleavage product was obtained.

[0091] Table 2 ...

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Abstract

The invention relates to a method for detecting chromosome abnormality before embryo implantation. Specifically, the detection accuracy is improved by adopting the steps of improving a single cell proliferation method and optimizing traditional library construction, and the false positive rate of a karyotype and a CNV is reduced.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to a method suitable for detecting abnormal chromosomes before embryo implantation. Background technique [0002] In recent years, affected by factors such as modern diet, environment, and living habits, infertility has become more and more common in my country, and the incidence rate in some areas is as high as 15%, which means that 1 in every 10 couples of childbearing age suffers from infertility . Especially after the country abolished the one-child policy and fully liberalized the two-child policy, the long-awaited families, remarried families, families who lost their only child, families who were abducted, and elderly families, etc. , the desire to have another child was widely stimulated, but it was followed by the troubles of infertility, which brought many new propositions to the diagnosis and treatment of infertility. [0003] Assisted reproductive technology can...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6806C12Q2531/113C12Q2525/191C12Q2521/301
Inventor 洪燕张超玄兆伶李大为梁峻彬陈重建
Owner ANNOROAD GENE TECH BEIJING
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