Kit for detecting folic acid utilization ability gene and detection method thereof

A gene detection and detection method technology, which is applied in the folic acid utilization ability gene detection kit and detection field, can solve the problems of low efficiency, waste of detection time, reagents, manpower and material resources, etc.

Inactive Publication Date: 2012-09-19
SUZHOU MUNICIPAL HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional genetic detection technology, each site must be detected separately, which greatly wastes the time, reagents, manpower and material resources of the detection, and the efficiency is low

Method used

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  • Kit for detecting folic acid utilization ability gene and detection method thereof
  • Kit for detecting folic acid utilization ability gene and detection method thereof
  • Kit for detecting folic acid utilization ability gene and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0178] Example 1: Gene detection of folic acid utilization ability in patients with congenital heart disease

[0179] 1. Test samples

[0180] The samples came from the DNA library of non-syndromic patients in the Reproductive and Genetic Center of Suzhou Municipal Hospital. They were all patients in the southern Jiangsu area, mainly in Suzhou, including 71 males and 54 females. All patient samples in the library signed the Informed Consent.

[0181] 2. Detection scheme

[0182] See the detection method of the specific embodiment.

[0183] 3. Test results

[0184] Judging by the difference in color between the wild-type and mutant peaks, in 88 samples of congenital heart disease, it was found that the distribution frequency of DHFR, MTR, SHMT, TCN2, and TYMS gene mutations was significantly different from that of the normal control group, see Appendix 4.

[0185]

[0186] Appendix 4: Distribution frequency of mutations in congenital heart disease and control samples (88...

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Abstract

The invention discloses a kit for detecting folic acid utilization ability gene and a detection method of the kit, relating to the technical field of gene detection, wherein the kit for detecting folic acid utilization ability gene comprises (1) a PCR (polymerase chain reaction) reaction reagent including dNTP, 5* and 10*PCR buffer liquid, Mg<2+> ion, de-ionized water, FastTaq enzyme, SNaPshot Mix mixed liquid; (2), a mixture obtained by mixing 16 pairs of PCR primers according to a certain proportion; (3), a mixture obtained by mixing 16 extended primers according to a certain proportion; (4), SAP enzyme for purification, Exon I enzyme and buffer liquid matched with the enzymes; (5), positive and negative control DNA with single locus subjected to homozygous or heterozygous mutation; (6), an instruction manual. The method provided by the invention is simple, high in flux, high in effect and low in cost, and is suitable for detecting folic acid utilization ability gene for Chinese population.

Description

Technical field: [0001] The invention relates to the technical field of gene detection, in particular to a folic acid utilization ability gene detection kit and detection method. Background technique: [0002] Folic acid is a B vitamin that cannot be synthesized by the human body. The folic acid ingested from food can only participate in many important biochemical metabolic reactions of the human body if it is reduced to tetrahydrofolate under the action of folic acid reductase. key cofactors. Therefore, lack of folic acid, fetuses, infants and young children will appear developmental defects and delays. The vigorous promotion of "folic acid fortification" among pregnant women has reduced birth defects to some extent. Pregnant women generally take one folic acid tablet (400 micrograms) per day to supplement folic acid. This dosage is sufficient for pregnant women with normal folic acid utilization capacity, but far from enough for pregnant women with insufficient folic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈瑛毛君王本敬李红
Owner SUZHOU MUNICIPAL HOSPITAL
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