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Method for detecting chicken tenderness character by using single nucleotide polymorphism (SNP) and primers

A single nucleotide polymorphism, tenderness technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as slow genetic progress and increased breeding costs

Inactive Publication Date: 2013-12-11
SICHUAN ANIMAL SCI ACAD
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Problems solved by technology

At present, the tenderness test can only be carried out after slaughtering. If the traditional method is used for breeding, it will not only increase the cost of breeding, but also slow the genetic progress.

Method used

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  • Method for detecting chicken tenderness character by using single nucleotide polymorphism (SNP) and primers
  • Method for detecting chicken tenderness character by using single nucleotide polymorphism (SNP) and primers
  • Method for detecting chicken tenderness character by using single nucleotide polymorphism (SNP) and primers

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Embodiment Construction

[0015] The present invention will be described in detail below in conjunction with specific embodiments.

[0016] The test flocks are mainly from 8 new high-quality broiler strains cultivated by Sichuan Daheng Poultry Breeding Co., Ltd., which are 5 pure lines S03, S02, S05, S01, D99 and 3 matching lines S01×D99, S01×S10 , S01×S05, the pure line has been selected for 3 generations, and 15 males and females were selected for each group, a total of 240 individuals were used as test materials. The whole feeding stage is managed by special personnel, single-cage feeding, consistent management and nutritional level, free feeding, and slaughtering when reaching the appropriate market weight (2kg). The blood sampling method was venous blood collection from chicken wings, and all blood samples were collected at 1 mL per chicken, anticoagulated with EDTA, and stored at -20°C.

[0017] Maximum shear value: Take the middle section of pectoralis muscle, put it flat into a plastic bag, re...

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Abstract

The invention discloses a method for detecting a chicken tenderness character by using single nucleotide polymorphism (SNP) and primers. The method comprises the following steps: carrying out SNPs detection on CDS (Coding Sequence) regions of CAPN1 (Calcium-Activated Neutral Protease) and CAST (Calpastatin) genes of a chicken, and detecting one SNP locus on an exon 6 of the CAPN1 gene by using a primer F1, wherein the SNP locus is concretely the 3535th position G->A on a GenBank sequence (GenBank NO:NC-006090.1); detecting one SNP locus on an exon 11 of the CAST gene by using a primer F2, wherein the SNP locus is concretely the 37752nd position A->T on a GenBank sequence (GenBank NO:NC-006127.2); and analyzing the relationship between different gene types of a single locus and the tenderness character in a high-quality broiler chicken.

Description

technical field [0001] The invention relates to a method and primers for detecting chicken tenderness traits by using molecular biology technology. The purpose of the present invention is to provide a method for detecting chicken tenderness traits with molecular biology technology, namely single nucleotide polymorphism. Background technique [0002] With the continuous improvement of living standards, people's demand for high-quality chickens is increasing day by day, and the production of high-quality chickens has gradually shifted from the past family-style sideline production to large-scale large-scale production. High-quality chicken mainly emphasizes meat quality. Local chicken breeds in my country generally have the characteristics of slow growth and good meat quality. Since the differences in genes and their expressions are the root cause of the diversity of biological traits, the differences in meat quality traits are fundamentally caused by the differences in genes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 张增荣蒋小松杜华锐杨朝武李晴云李雯邱莫寒宋小燕胡陈明夏波
Owner SICHUAN ANIMAL SCI ACAD
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