Method for detecting chicken tenderness character by using single nucleotide polymorphism (SNP) and primers

A single nucleotide polymorphism, tenderness technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as slow genetic progress and increased breeding costs
CN103436632AInactive Publication Date: 2013-12-11SICHUAN ANIMAL SCI ACAD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SICHUAN ANIMAL SCI ACAD
Publication Date
2013-12-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting a chicken tenderness character by using single nucleotide polymorphism (SNP) and primers. The method comprises the following steps: carrying out SNPs detection on CDS (Coding Sequence) regions of CAPN1 (Calcium-Activated Neutral Protease) and CAST (Calpastatin) genes of a chicken, and detecting one SNP locus on an exon 6 of the CAPN1 gene by using a primer F1, wherein the SNP locus is concretely the 3535th position G->A on a GenBank sequence (GenBank NO:NC-006090.1); detecting one SNP locus on an exon 11 of the CAST gene by using a primer F2, wherein the SNP locus is concretely the 37752nd position A->T on a GenBank sequence (GenBank NO:NC-006127.2); and analyzing the relationship between different gene types of a single locus and the tenderness character in a high-quality broiler chicken.
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Description

technical field

[0001] The invention relates to a method and primers for detecting chicken tenderness traits by using molecular biology technology. The purpose of the present invention is to provide a method for detecting chicken tenderness traits with molecular biology technology, namely single nucleotide polymorphism. Background technique

[0002] With the continuous improvement of living standards, people's demand for high-quality chickens is increasing day by day, and the production of high-quality chickens has gradually shifted from the past family-style sideline production to large-scale large-scale production. High-quality chicken mainly emphasizes meat quality. Local chicken breeds in my country generally have the characteristics of slow growth and good meat quality. Since the differences in genes and their expressions are the root cause of the diversity of biological traits, the differences in meat quality traits are fundamentally caused by the differences in genes...

Claims

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