Vegetation and extraction mixture for promoting growth and coccidiosis resistance of broiler chickens and feed and application thereof

Abstract

The invention discloses a plant extract mixture for promoting growth of broiler chicken and resisting coccidiosis and feed and application thereof, and the plant extract mixture comprises the following components in parts by weight: 3-6 parts of ferulic acid, 6-11 parts of shikimic acid, 2-5 parts of mixed-source terpene, 9-14 parts of chlorogenic acid, 11-14 parts of rutin and 5-8 parts of chicoric acid. According to the invention, substances extracted from plants are adopted to prevent and treat poultry coccidiosis, the composition has the characteristics of greenness and safety, and through the synergistic interaction of the components, the composition can better resist coccidiosis infection, improve the growth performance after coccidiosis infection, and reduce the lesion coefficient of intestinal tracts after coccidiosis infection and the number of coccidiosis oocysts in excrement.

Description

Technical Field

[0001] The invention relates to the technical field of feed, in particular to a plant extract mixture for promoting broiler growth and resisting coccidiosis, and feed and application thereof. Background Art

[0002] Coccidiosis is a parasitic disease of poultry that causes millions of dollars in economic losses to the world poultry industry each year and is widely considered to be one of the greatest threats to the poultry industry. The disease is caused by apicomplexan parasites of the genus Eimeria and can lead to impaired growth and feed utilization. Currently, there are seven different species of Eimeria that invade different parts of the gastrointestinal tract of poultry and cause considerable damage to the epithelial layer of the gastrointestinal tract. The effects of the damage vary, from local destruction of the mucosal barrier and other tissues to systemic effects, which in more severe cases can lead to death. However, commercial broilers are not usually vaccinated against coccidiosis. Currently, coccidiosis is mainly prevented by taking Western medicine, which on the one hand can lead to drug resistance in animals and on the other hand can affect human health. Therefore, there is an urgent need to develop new control methods that can fully prevent various coccidiosis infections and avoid their adverse effects on production performance. Summary of the invention

[0003] The purpose of the present invention is to solve the above problems and provide a plant extract mixture for promoting the growth of broiler chickens and resisting coccidiosis, and a feed and application thereof.

[0004] The purpose of the present invention is achieved through the following technical scheme: a plant extract mixture for promoting broiler growth and resisting coccidiosis, comprising the following components in parts by weight: 3-6 parts of ferulic acid, 6-11 parts of shikimic acid, 2-5 parts of mixed terpenes, 9-14 parts of chlorogenic acid, 11-14 parts of rutin, and 5-8 parts of chicoric acid.

[0005] Furthermore, the plant extract mixture for promoting broiler growth and resisting coccidiosis comprises the following components in parts by weight: 5 parts of ferulic acid, 8 parts of shikimic acid, 3 parts of mixed terpenes, 10 parts of chlorogenic acid, 11 parts of rutin, and 6 parts of chicoric acid.

[0006] The present invention also proposes the use of the plant extract mixture in preparing chicken feed for promoting broiler growth and resisting coccidiosis.

[0007] The present invention also provides a chicken feed, which contains the plant extract mixture.

[0008] Compared with the prior art, the present application has the following beneficial effects: the present invention adopts substances derived from plant extracts to prevent and treat poultry coccidiosis, which is green and safe, and through the synergistic effect between the components, it can better resist coccidiosis infection, improve the growth performance after coccidiosis infection, and reduce the pathological coefficient of the intestine after coccidiosis infection and the number of coccidia oocysts in feces.

[0009] Some additional features of the present application may be described in the following description. Some additional features of the present application will be apparent to those skilled in the art through inspection of the following description or understanding of the production or operation of the embodiments. The features disclosed in the present application may be realized and achieved through the practice or use of various methods, means and combinations of the specific embodiments described below. DETAILED DESCRIPTION

[0010] Example 1

[0011] The plant extract mixture disclosed in this embodiment comprises the following components in parts by weight: 5 parts of ferulic acid, 8 parts of shikimic acid, 3 parts of mixed terpenes, 10 parts of chlorogenic acid, 11 parts of rutin, and 6 parts of chicoric acid.

[0012] Example 2

[0013] The plant extract mixture disclosed in this embodiment comprises the following components in parts by weight: 3 parts of ferulic acid, 10 parts of shikimic acid, 5 parts of mixed terpenes, 14 parts of chlorogenic acid, 12 parts of rutin, and 5 parts of chicoric acid.

[0014] Example 3

[0015] The plant extract mixture disclosed in this embodiment comprises the following components in parts by weight: 6 parts of ferulic acid, 11 parts of shikimic acid, 2 parts of mixed terpenes, 9 parts of chlorogenic acid, 10 parts of rutin, and 7 parts of chicoric acid.

[0016] Comparative Example 1

[0017] The difference between this comparative example and the plant extract mixture in Example 1 is that the mixed terpenes are 1 part, the chlorogenic acid is 5 parts, and the chicoric acid is 3 parts, and the other components and contents remain unchanged.

[0018] Comparative Example 2

[0019] The difference between this comparative example and the plant extract mixture in Example 1 is that the mixed terpenes are 8 parts, the chlorogenic acid is 18 parts, and the chicoric acid is 10 parts, and the other components and contents remain unchanged.

[0020] Comparative Example 3

[0021] The difference between this comparative example and the plant extract mixture in Example 1 is that the mixed terpenes are 12 parts, the chlorogenic acid is 20 parts, and the chicoric acid is 13 parts, and the other components and contents remain unchanged.

[0022] Comparative Example 4

[0023] The difference between this comparative example and the plant extract mixture in Example 1 is that this comparative example only contains 8 parts of shikimic acid, 3 parts of mixed terpenes and 6 parts of chicoric acid.

[0024] Comparative Example 5

[0025] The difference between this comparative example and the plant extract mixture in Example 1 is that this comparative example only contains 10 parts of chlorogenic acid, 5 parts of ferulic acid and 11 parts of rutin.

[0026] Comparative Example 6

[0027] The difference between this comparative example and the plant extract mixture in Example 1 is that in this comparative example, 3 parts of mixed terpenes in Example 1 are replaced by 3 parts of phloretin, and 10 parts of chlorogenic acid are replaced by 10 parts of rhein.

[0028] Comparative Example 7

[0029] The difference between this comparative example and the plant extract mixture in Example 1 is that in this comparative example, 5 parts of ferulic acid in Example 1 are replaced by 5 parts of punicalagin, and 11 parts of rutin are replaced by 11 parts of Lycium barbarum polysaccharides.

[0030] Comparative Example 8

[0031] The plant extract mixture of this comparative example comprises the following components in parts by weight: 10 parts of phloretin, 15 parts of EGCG, 8 parts of rhein, 4 parts of total saponins, 7 parts of squalene, and 12 parts of wolfberry polysaccharides.

[0032] Comparative Example 9

[0033] The plant extract mixture of this comparative example comprises the following components in parts by weight: 6 parts of erucicola, 9 parts of punicalagin, 11 parts of mangostin, 8 parts of rosemary, 12 parts of phloridzin, and 8 parts of eucommia polysaccharide.

[0034] The plant extract mixtures in the above Examples 1-3 and Comparative Examples 1-9 were subjected to performance tests, and the test methods were as follows:

[0035] Take 13 equal portions of basic feed, take the plant extract mixtures in the above Examples 1-3 and Comparative Examples 1-9 respectively, add them to the 1st to 12th portions of basic feed at a weight ratio of 0.35:1000, mix them evenly, and prepare chicken feed, that is, add 0.35g of plant extract mixture to every 1kg of basic feed. No plant extract mixture is added to the 13th portion of basic feed. The basic feed is prepared according to the nutritional standard of broiler compound feed (GB / T 5916-2020 Compound feed for laying hens and broilers).

[0036] 260 one-day-old fast-growing white-feathered broilers were randomly divided into 13 test groups. The 1st to 3rd test groups were respectively fed with the above-mentioned chicken feed supplemented with the plant extract mixture in Examples 1-3, the 4th to 12th test groups were respectively fed with the above-mentioned chicken feed supplemented with the plant extract mixture in Comparative Examples 1-9, and the 13th test group was fed with the above-mentioned basic feed.

[0037] The experimental broilers were raised in cages for 42 days. During the entire trial, all broilers had free access to water and feed. In the initial stage (d1-d5), the temperature in the breeding room was maintained at 32-34°C, and it was reduced by 2°C every week until the final temperature range of 22-24°C was reached. The relative humidity was always maintained at 40-60%. During 1-10d, the broilers were continuously exposed to light, followed by 17h of light and 7h of darkness. Growth performance was statistically analyzed during the trial, and feces were collected four days after the trial to measure digestibility.

[0038] Table 1 shows the average digestibility of nutrients in broiler chickens in each test group from 1 to 35 days:

[0039]

[0040] Table 1

[0041] Table 2 shows the production performance of broilers in each test group from 1 to 35 days:

[0042]

[0043] Table 2

[0044] As can be seen from Tables 1 and 2, compared with the broilers fed with the basic feed in the experimental group 13, the nutrient digestibility and production performance of the broilers in groups 1-12 fed with the chicken feed added with the plant extract mixture were improved to a certain extent, and the nutrient digestibility and production performance of the broilers in groups 1-3 fed with the plant extract mixture added in the present application were significantly improved, among which the nutrient digestibility and production performance of the broilers in the first group of experiments were the best. Although the nutrient digestibility and production performance of the broilers in groups 4-12 fed with the plant extract mixture added in the comparative examples 1-9 were improved, they were not significant.

[0045] On the 36th day, broilers in each experimental group were gavaged with 1 mL of coccidia oocysts (the number of coccidia oocysts was 2.5×105 / mL, containing tender, toxic, pileated, and giant Eimeria coccidia). On the 42nd day, 6 chickens were randomly selected from each experimental group and euthanized for lesion scoring associated with coccidia challenge. The three segments of the intestine (duodenum, jejunum, and cecum) were evaluated using the standard Johnson and Reid (1970) method, with scores ranging from 0 (no gross lesions) to 4 (most severe lesions). Fecal specimens were collected from each breeding cage 3-7 days after inoculation of coccidia. Samples were stored in sealed plastic bags at 4°C until the oocyst count was evaluated. Samples from the same group were mixed, diluted, and the oocysts were counted under a microscope using an oocyst counting plate (JA Whitlock & Co., Eastwood, NSW, Australia), and then expressed as the number of oocysts per gram of excrement.

[0046] Table 3 shows the production performance of broilers in each test group from 36 to 42 days:

[0047]

[0048] Table 3

[0049] As can be seen from Table 3, compared with the broilers fed with the basic feed in the experimental group 13, the broilers in groups 1-3 fed with the plant extract mixture added in the present application had a more obvious improvement in production performance after the challenge, and the mortality rate was also significantly reduced, among which the effect of the experimental group 1 was the most significant. The production performance of the broilers in groups 4-12 fed with the chicken feed added with the plant extract mixture in the comparative examples 1-9 was improved to a certain extent, and the mortality rate was also reduced to a certain extent, but both were not very obvious.

[0050] Table 4 shows the pathological index of the duodenum, jejunum and cecum of broilers in each experimental group:

[0051]

[0052] Table 4

[0053] As can be seen from Table 4, compared with the broilers fed with the basic feed in the experimental group 13, the 1-3 groups of broilers fed with the plant extract mixture added in the present application had significantly lower pathological indexes of the duodenum, jejunum and cecum. The 4-12 groups of broilers fed with the chicken feed added with the plant extract mixture in the comparative examples 1-9 had lower pathological indexes of the duodenum, jejunum and cecum, but not significantly.

[0054] Table 5 shows the number of oocysts per gram of excrement in the feces of broiler chickens in each test group:

[0055]

[0056]

[0057] Table 5

[0058] As can be seen from Table 5, compared with the broilers fed with the basic feed in the experimental group 13, the number of oocysts in the excrement of the broilers in groups 1-3 fed with the plant extract mixture added in the present application was significantly reduced. The number of oocysts in the excrement of the broilers in groups 4-12 fed with the chicken feed added with the plant extract mixture in the comparative examples 1-9 was reduced, but not significantly.

[0059] It can be seen from the above experiments that the various components in the plant extract mixture of the present application can produce a synergistic effect, so that the plant extract mixture of the present application has better antioxidant, anti-inflammatory and antiviral effects, and the chicken feed added with the plant extract mixture in the present application can significantly promote the growth performance of broilers, and reduce the pathological coefficient of the intestine after coccidia infection and the number of coccidia oocysts in feces. Although the various components in the plant extract mixture in Comparative Examples 1-9 also have certain antioxidant and anti-inflammatory effects, the various components cannot play a synergistic effect, so the performance effect on broiler growth performance and anti-coccidial performance is not good.

[0060] It should be noted that all features disclosed in this specification, or steps in all methods or processes disclosed, except for mutually exclusive features and / or steps, can be combined in any manner.

[0061] In addition, the above specific embodiments are exemplary, and those skilled in the art can come up with various solutions inspired by the disclosure of the present invention, and these solutions also belong to the disclosure scope of the present invention and fall within the protection scope of the present invention. Those skilled in the art should understand that the description of the present invention is illustrative and does not constitute a limitation of the claims. The protection scope of the present invention is defined by the claims and their equivalents.

Claims

1. A plant extract mixture for promoting broiler growth and resisting coccidiosis, characterized in that: The invention comprises the following components in parts by weight: 3-6 parts of ferulic acid, 6-11 parts of shikimic acid, 2-5 parts of mixed terpenes, 9-14 parts of chlorogenic acid, 11-14 parts of rutin and 5-8 parts of chicoric acid.

2. The plant extract mixture for promoting broiler growth and resisting coccidiosis according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 5 parts of ferulic acid, 8 parts of shikimic acid, 3 parts of mixed terpenes, 10 parts of chlorogenic acid, 11 parts of rutin and 6 parts of chicoric acid.

3. Use of the plant extract mixture according to claim 1 or 2 in preparing chicken feed for promoting broiler growth and resisting coccidiosis.

4. A chicken feed, characterized in that: Contains the plant extract mixture according to claim 1 or 2.

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