Culture method for improving grease content of saccharomyces cerevisiae by maintaining ultralow-concentration organic carbon source and application of culture method

A culture method and technology of Saccharomyces cerevisiae, applied in the biological field, can solve problems such as safety disputes, difficulty in commercial application, and no significant increase in oil production, and achieve the effect of promoting oil accumulation

Active Publication Date: 2021-01-29
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But oil production has not been significantly improved (Kyung Ok Yu, et al. Development of a Saccharomyces cerevisiae strain for increasing the accumulation of triacylglycerol as a microbial oil feedstock for biodiesel production using glycerol as a substrate. Biotechnology & Bioengineering, 2012), 110( 347), and the safety of genetically engineered microorganisms used to produce food and other raw materials is still hugely controversial. Therefore, it is difficult to realize commercial application of genetically engineered microorganisms to produce food raw materials in the short term

Method used

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  • Culture method for improving grease content of saccharomyces cerevisiae by maintaining ultralow-concentration organic carbon source and application of culture method
  • Culture method for improving grease content of saccharomyces cerevisiae by maintaining ultralow-concentration organic carbon source and application of culture method
  • Culture method for improving grease content of saccharomyces cerevisiae by maintaining ultralow-concentration organic carbon source and application of culture method

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Experimental program
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Effect test

Embodiment 1

[0056] Under the conditions of different organic carbon source concentrations, the growth and oil content of Saccharomyces cerevisiae were measured, and the specific implementation steps were as follows:

[0057] Using glucose as the organic carbon source, Saccharomyces cerevisiae was cultivated by continuous flow feeding. During the cultivation process, the concentration of glucose in the culture solution was measured in real time with a biosensor, and the flow velocity of the glucose mother solution was adjusted to make the concentration of glucose in the culture solution Maintain at 0.1g / L, 0.5g / L, 1g / L, 10g / L, 20g / L, 50g / L respectively, measure the biomass (weight method) and oil content of Saccharomyces cerevisiae in the culture medium after continuous culture for 4 days (chloroform-methanol method). Other culture conditions are: inoculum size 5%, yeast extract powder 1%, peptone 2%, temperature 28°C, rotation speed 1000rpm, air ventilation volume 5vvm, pH6. It should be...

Embodiment 2

[0060] In the case of ultra-low concentration of organic carbon source and nitrogen deficiency, the comparison of the effects on the growth and oil content of Saccharomyces cerevisiae, the specific implementation steps are as follows:

[0061] Using glucose as the organic carbon source, Saccharomyces cerevisiae was cultivated by continuous flow feeding. During the cultivation process, the concentration of glucose in the culture solution was measured in real time with a biosensor, and the flow velocity of the glucose mother solution was adjusted to make the concentration of glucose in the culture solution Maintain at 0.5g / L. At the same time, taking the culture group without adding nitrogen source as a control, Saccharomyces cerevisiae was cultured by adding 150 g / L glucose once. After continuous cultivation for 4 days, the biomass (weight method) and oil content (chloroform-methanol method) of Saccharomyces cerevisiae in the culture medium were measured. Other culture conditi...

Embodiment 3

[0064] Under anaerobic and aerobic culture conditions, the effects of ultra-low concentration organic carbon sources on the growth and oil content of Saccharomyces cerevisiae were studied. The specific implementation steps are as follows:

[0065] Using glucose as the organic carbon source, Saccharomyces cerevisiae was cultured by continuous feed feeding under anaerobic and aerobic conditions respectively. During the cultivation process, a biosensor was used to measure the concentration of glucose in the culture medium in real time and adjust the concentration of the glucose mother solution. The flow rate was used to maintain the glucose concentration in the culture medium at 0.5 g / L. After continuous cultivation for 4 days, the biomass (weight method) and oil content (chloroform-methanol method) of Saccharomyces cerevisiae in the culture medium were measured. Other culture conditions are: inoculum size 5%, yeast extract powder 7%, peptone 0%, temperature 38° C., rotation spee...

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Abstract

The invention provides a culture method for increasing the grease content of saccharomyces cerevisiae by maintaining an ultralow-concentration organic carbon source and application of the culture method and belongs to the technical field of biology. Research finds that in the fermentation culture process of saccharomyces cerevisiae, the concentration of an organic carbon source in a fermentation culture solution is continuously maintained at an ultra-low level by controlling the supplementing amount of the organic carbon source, and in the state, activity of enzymes related to ethanol synthesis in saccharomyces cerevisiae cells is greatly reduced due to lack of an acting substrate, so activity of the enzymes related to ethanol synthesis in saccharomyces cerevisiae cells is improved. However, activity of enzymes related to fatty acid synthesis is not obviously influenced, so the flow direction of an organic carbon source deviates to fatty acid synthesis, and finally, a large amount of grease in saccharomyces cerevisiae cells is accumulated. The method is advantaged in that raw materials are low in cost, the culture method is simple and easy to implement, industrial production and application of the method are facilitated, and therefore the method has good actual production and application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cultivation method for increasing the oil content of Saccharomyces cerevisiae by maintaining an ultra-low concentration organic carbon source and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Saccharomyces cerevisiae, which is mainly used in the brewing industry and pastry food processing industry, is also the main object of basic and applied research, and is widely used in food, medicine and other fields. The oil of Saccharomyces cerevisiae is rich in palmitoleic acid (hexadecenoic acid, C16:1), a rare and expensive omega-7 monounsaturated fatty a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12P7/64C12R1/865
CPCC12N1/18C12P7/6463Y02E50/10
Inventor 周文俊刘天中纪晓彤高莉丽
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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