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Microalgae cultivation method and grease production method

A technology of microalgae and algae liquid, applied in the field of microalgae cultivation, can solve the problems of high energy consumption, cumbersome process, and unsuitability for large-scale industrial production

Inactive Publication Date: 2014-09-17
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is not suitable for large-scale industrial production, because it is cumbersome and energy-intensive to separate the microalgae from the nitrogen-rich medium and put them into the nitrogen-deficient medium
[0006] It has been reported in the literature that by controlling the concentration of nitrogen source to increase the biomass and oil content of microalgae at the same time, these methods only control the concentration of nitrogen source and do not make the microalgae in a state of nitrogen deficiency. Under optimal conditions to simultaneously increase microalgae growth rate and lipid content, these methods are only applicable to specific algae species

Method used

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  • Microalgae cultivation method and grease production method
  • Microalgae cultivation method and grease production method
  • Microalgae cultivation method and grease production method

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The cultivation of Chlorella is carried out by adopting the two-stage expanded basic technological process. Primary inoculation culture: 1L glass triangular flask is filled with about 700ml of culture medium, the medium is BG11 medium (Table 1), the primary inoculation medium uses urea as nitrogen fertilizer, the concentration is 0.3g / L, and the light and temperature are controlled appropriately. Inject 2% (v / v) CO2 air mixture for aeration culture, and the mixture is purified by filter membrane. Detect the OD value of the algae liquid. When the OD value of the algae liquid reaches more than 5, the secondary expansion culture can be carried out. Secondary expanded culture adopts the method of the present invention: expanded culture is carried out in 5L glass Erlenmeyer flask, packs 3~4L culture medium (culture medium mother liquor is through autoclaving high temperature sterilization or boiling disinfection, culture water adopts the tap water of filter disinfection), ac...

Embodiment 2

[0049] The culture of chlorella, method is the same as example 1, and difference is that the nitrogenous fertilizer that adds is urea, and addition is 9mg / L.d. Harvest after continuous cultivation for 20 days, its growth curve is shown in figure 1 .

Embodiment 3

[0051] The cultivation of Scenedesmus, method is with example 1. Harvest after continuous cultivation for 20 days, its growth curve is shown in figure 2 .

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Abstract

The invention relates to a microalgae cultivation method and a grease production method. The cultivation method comprises the following steps: a nitrogen fertilizer is provided for microalgae for many times; at least for one time, the nitrogen fertilizer is added when the nitrogen fertilizer in a microalgae suspension is consumed by microalgae until the microalgae is in a nitrogen-deficiency simulation state; and the cultivation time lasts for more than 10 days. By the cultivation method, normal growth of microalgae can be guaranteed, and the microalgae can accumulate much grease.

Description

technical field [0001] The present invention relates to a method for culturing microalgae and a method for producing oil. Background technique [0002] Microalgae are a class of low-level plants that grow in water with a wide variety and are extremely widely distributed. It is a cell factory driven by sunlight, and absorbs CO through efficient photosynthesis of microalgae cells. 2 , convert light energy into chemical energy of carbohydrates such as fat or starch, and release O 2 . The use of microalgae to produce bioenergy and chemicals can simultaneously achieve "replacing fossil energy and reducing CO 2 The three purposes of "emission, purification of waste gas and sewage". The advantages of "microalgae biotechnology" lie in the following aspects: ① Microalgae is the original plant with the highest photosynthetic efficiency. Compared with crops, the yield per unit area is dozens of times higher Microalgae is also the fastest-growing plant in nature, usually within 24 ho...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12R1/89
Inventor 荣峻峰周旭华朱俊英黄绪耕
Owner CHINA PETROLEUM & CHEM CORP
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