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Haematococcus pluvialis jnu35 with high astaxanthin production and its cultivation method and application

A technology of Haematococcus pluvialis and astaxanthin, which is applied in the field of Haematococcus pluvialls JNU35 and its cultivation, can solve the problems of complex induction conditions, difficulty in achieving biomass of Haematococcus pluvialls, and low astaxanthin production , to achieve the effect of increasing biomass and astaxanthin content, good industrial production prospects, and high operability

Active Publication Date: 2022-04-26
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the biggest problem in the production of astaxanthin from Haematococcus pluvialis in industry is mainly the characteristics of algae species. Due to the influence of phenotypic and genetic differences of different Haematococcus pluvialis strains, most research results show that the photosynthetic autotrophic culture However, it is difficult for Haematococcus pluvialis to achieve a high biomass, and there are problems such as low astaxanthin production, easy pollution, slow growth, and complicated induction conditions.

Method used

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  • Haematococcus pluvialis jnu35 with high astaxanthin production and its cultivation method and application
  • Haematococcus pluvialis jnu35 with high astaxanthin production and its cultivation method and application
  • Haematococcus pluvialis jnu35 with high astaxanthin production and its cultivation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Isolation and Identification of Embodiment 1 JNU35

[0053] 1. Algae sample collection, separation and purification

[0054] The improved BG-11 medium in this example is to add NaNO with a nitrogen concentration of 18mmol / L to the above-mentioned nitrogen-free improved BG-11 medium 3 be made of.

[0055] Take the water sample from the small sand pond beside the Jiageng stream in Gongga Mountain, Sichuan, at an altitude of 3948 meters, back to the laboratory, take 1mL water sample, add it to a 100mL triangular flask containing 50mL of improved BG-11 medium, and set up 5 In parallel, culture under continuous light at 25°C for 5 days, then transfer 1 mL of the culture sample to a 1.5 mL sterile EP tube under aseptic operation every day, observe whether there are algae cells growing, and take 5 μL of algae containing the target algae smeared on the solid plate of improved BG-11 medium (solid medium was added to agar 20g / L), and cultured under continuous light at 25°C. Af...

Embodiment 2

[0072] The influence of embodiment 2 different initial nitrogen concentrations on JNU35

[0073] 1. Preparation of Algae Seeds

[0074] Firstly, the aseptic JNU35 algae species preserved in the conical flask were amplified step by step into a glass columnar photobioreactor with a light path of 10 cm. After the algae cultured to the logarithmic growth phase are settled and washed with sterile water, the algae cells are collected as the secondary algae species, and then the subsequent culture is carried out.

[0075] 2. Changes in biomass under different initial nitrogen concentrations

[0076] With the initial inoculum concentration of OD750=0.50~0.90, in The glass columnar photobioreactor was cultivated, and a two-step culture strategy was adopted. In the first stage, the improved BBM medium was selected, and CO(NH 2 ) 2 As the nitrogen source, set different initial nitrogen concentrations (3mmol / L, 9mmol / L and 18mmol / L), and the 24h single-side light intensity is 100-200...

Embodiment 3

[0082] The influence of embodiment 3 different light intensities on JNU35

[0083] exist The glass columnar photobioreactor was cultivated, inoculated with the initial concentration of OD750=0.50~0.90, the medium of the first stage was the improved BBM medium, and the nitrogen source was CO(NH 2 ) 2 (Nitrogen concentration is 18mmol / L), 24h single side light intensity 100~200μmol·m -2 ·s -1 Continuous light, culture temperature 25~30℃, feed with 1% CO 2 compressed air for agitation. After 15 days of culture, transfer to the second stage, transfer the algae cells to the nitrogen-free BBM medium, and set the light intensity on one side for 24 hours to 300 μmol·m -2 ·s -1 and double-sided light intensity 300μmol·m -2 ·s -1 Continuous light, other conditions are consistent with the first stage. Comparison of the effects of light intensity on JNU35 growth and astaxanthin accumulation.

[0084] Such as Figure 5 It shows that the biomass of JNU35 reaches 5.1g / L after 15 ...

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Abstract

The invention provides Haematococcus pluvialis JNU35 with high astaxanthin production and its cultivation method and application. The algae strain is named Haematococcus pluvialis JNU35, and it was deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms in Beijing, China on September 7, 2018, with the preservation number CGMCC No.16438. At the same time, it provides a cultivation method of the Haematococcus pluvialis JNU35, which can quickly accumulate biomass. The present invention also provides a method for inducing astaxanthin in Haematococcus pluvialis JNU35, through the innovation and optimization of the two-step method strategy, it effectively balances the synchronous and rapid accumulation of biomass and astaxanthin content, greatly improving The biomass of Haematococcus pluvialis and the content of astaxanthin are increased, the cost of astaxanthin production can be significantly reduced, and it has important application value in actual production and has a good prospect for industrial production.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to Haematococcus pluvialis JNU35 with high astaxanthin production and its cultivation method and application. Background technique [0002] Astaxanthin is a red natural carotenoid, which has a powerful ability to scavenge oxygen free radicals. There are currently three recognized sources of natural astaxanthin: extraction from crustaceans, production by yeast, and production by microalgae, and Haematococcus pluvialis is currently known to have the highest content of natural astaxanthin The content of astaxanthin is usually 1.5-3.6%, the highest can reach 5% of dry weight, and it is considered as a concentrated product of natural astaxanthin. Therefore, the natural algal source astaxanthin has a broad market prospect, and the efficient production of astaxanthin by using Haematococcus pluvialis has always been a hot spot in the field of phycology research and produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P23/00C12R1/89
CPCC12N1/12C12P23/00C12R2001/89C12N1/125
Inventor 张成武黄罗冬高保燕吴曼曼
Owner JINAN UNIVERSITY
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