A compound with induced growth promoting effect and its application in medicine and fertilizer

Abstract

This invention relates to a compound with resistance-inducing and growth-promoting effects and its application in pesticide-fertilizer systems. The main active ingredient of the compound is methyl ferulic acid, with an effective concentration of 0.05 μg / mL to 0.5 μg / mL, used to induce plant disease resistance and promote plant growth. This invention also discloses the application of methyl ferulic acid in pesticide-fertilizer systems, comprising pesticide active ingredients, methyl ferulic acid, wetting agents, dispersants, thickeners, defoamers, stabilizers, and antifreeze agents; the effective concentration of methyl ferulic acid is 0.05 μg / mL to 0.5 μg / mL. This invention combines pesticide activity with nutrient supply, forming a dual-effect composition of "disease prevention + nutrition," solving the cumbersome problem of multiple applications of pesticides and fertilizers in traditional agriculture, significantly reducing the number of applications and labor costs, and improving field management efficiency.

Description

Technical Field

[0001] This invention relates to the fields of agricultural biology and plant protection technology, and in particular to a compound with resistance-inducing and growth-promoting effects and its application in pesticides and fertilizers. Background Technology

[0002] In modern agricultural production systems, the demands for improved crop quality and yield, green disease control, and sustainable agricultural ecological development are increasingly urgent. Traditional field management models are no longer adequate to meet the core requirements of current industrial upgrading. For a long time, the two core aspects of agricultural production—disease control and nutrient supply—have been isolated, typically requiring the separate application of chemical pesticides and fertilizers. This multi-application approach has several significant drawbacks: First, the operation is cumbersome; multiple sprayings not only significantly increase labor input and costs but also raise the risk of mechanical damage to crop stems and leaves from agricultural machinery operations, significantly reducing field management efficiency. This problem is particularly pronounced in large-scale planting scenarios, where it significantly restricts production benefits. Second, excessive and frequent application of chemical pesticides and fertilizers can easily lead to ecological problems such as soil physicochemical degradation and water and air pollution. It may also result in pesticide residues exceeding standards in agricultural products, affecting their quality, safety, and market competitiveness, which is seriously contrary to the development orientation of green and ecological agriculture.

[0003] From the perspective of disease control, rice sheath blight and apple anthracnose leaf blight are common diseases in my country's grain, oil, and fruit industries, posing a serious threat to crop yield and quality. Currently, the control of these diseases mainly relies on chemical fungicides, such as triazole and methoxyacrylate agents like tebuconazole and azoxystrobin. Although these fungicides can directly inhibit the growth and reproduction of pathogens, their mechanism of action is relatively simple. Long-term use in isolation can easily induce drug resistance in pathogens, leading to a gradual decline in the effectiveness of the fungicides and a shortened lifespan of control. Furthermore, chemical fungicides only target pathogens and cannot activate the plant's own immune defense system, making it difficult to achieve long-term control of diseases. Once the effective period of the fungicide ends, crops still face a high risk of infection. In addition, the high concentrations of some chemical fungicides further exacerbate the ecological and environmental risks and the safety hazards to agricultural products.

[0004] In the field of plant growth regulation and nutrient supply, existing technologies mostly focus on the research and application of single-function products. For example, regulators such as 28-homobrassinolide only focus on promoting crop growth and enhancing stress resistance; conventional macro- and micronutrient fertilizers only provide the nutrients required for crop growth; and immune inducers are mainly used to activate the expression of plant disease-resistant genes. These single-function products cannot meet the synergistic needs of "disease prevention and growth promotion" during crop growth. Farmers need to purchase and apply different types of products multiple times, which increases production costs and reduces operational efficiency.

[0005] Methyl ferulic acid, a naturally occurring compound widely found in plants, offers new avenues for the development of multifunctional agricultural products based on its immune-inducing and growth-promoting activities. However, systematic research and industrial application data are still lacking regarding its effective concentration threshold, compatibility with pesticides and nutrients, and synergistic mechanisms in multiple crop lines. Summary of the Invention

[0006] The present invention aims to solve the above-mentioned problems. The first aspect of the present invention provides a compound with the effect of inducing resistance and promoting growth. The main active ingredient of the compound is methyl ferulic acid, and the effective concentration of methyl ferulic acid is 0.05 μg / mL to 0.5 μg / mL, which is used to induce plant disease resistance and promote plant growth.

[0007] Secondly, the present invention provides a medicated fertilizer with disease resistance and growth promotion effects, the medicated fertilizer comprising pesticide active ingredients, methyl ferulic acid, wetting agent, dispersant, thickener, defoamer, stabilizer and antifreeze agent; the effective concentration of methyl ferulic acid is 0.05 μg / mL to 0.5 μg / mL.

[0008] Furthermore, the concentration of methyl ferulic acid is 0.1 μg / mL.

[0009] Furthermore, the active pesticide ingredient includes tebuconazole, azoxystrobin, avermectin, fluopyram, or other pesticide ingredients.

[0010] Thirdly, the present invention provides a method for preparing a medicated fertilizer with resistance-inducing and growth-promoting effects, comprising the following steps: S1: Weigh the mass of each component of the pesticide according to its weight percentage; S2: Add pesticide components and an appropriate amount of water to a sand mill containing zirconia beads for sand milling until D90≤5μm. According to the properties of the pesticide active ingredient, condensate water is passed through to lower the temperature to obtain a pesticide solution. S3: Add wetting agent, dispersant, thickener, defoamer, stabilizer, antifreeze and methyl ferulic acid to the pesticide solution and mix well.

[0011] Fourthly, the application of fertilizers with growth-promoting and resistance-inducing effects as described in the second aspect or fertilizers prepared by the method in the third aspect in promoting plant growth.

[0012] Furthermore, the plants mentioned include fruit trees, vegetables, grain and oil crops, etc.

[0013] Preferably, the fruit tree is an apple, the vegetables are chili peppers and loofahs, and the grain and oil crop is rice.

[0014] Fifthly, the application of any of the fertilizers with growth-promoting and resistance-inducing effects described in the second aspect or the fertilizers prepared by the method in the third aspect in the prevention and control of rice sheath blight.

[0015] The sixth aspect is the application of any of the fertilizers with inducing resistance and promoting growth as described in the second aspect or the fertilizers prepared by the method in the third aspect in the control of root-knot nematodes.

[0016] The present invention has the following beneficial effects: 1. This invention combines plant growth regulation function with pesticide activity and nutrient supply to form a multi-effect combination of "disease prevention + nutrition", which solves the cumbersome problem of applying pesticides and fertilizers in multiple times in traditional agriculture, greatly reduces the number of applications and labor costs, and improves field management efficiency.

[0017] 2. Methyl ferulic acid can significantly promote plant growth and induce disease resistance at extremely low concentrations (0.05–0.5 μg / mL), with the 0.1 μg / mL concentration showing particularly good cost-effectiveness. This composition reduces the application of chemical pesticides and fertilizers, lowers the risk of pollution to the environment and agricultural products, and meets the requirements of green agriculture and sustainable development.

[0018] 3. When methyl ferulic acid is combined with pesticide components such as tebuconazole·oxamyl ester and abamectin·fluopyram, it exhibits a synergistic effect in controlling diseases such as rice sheath blight and root-knot nematodes in loofah. At the same time, it systematically enhances plant immunity by activating the expression of plant disease resistance-related genes (such as the PR family), achieving the dual effects of "disease prevention" and "growth promotion".

[0019] 4. This composition is suitable for various plant types, including fruit trees, vegetables, and grain and oil crops, and shows significant growth-promoting and disease-resistant effects, especially on crops such as apples, peppers, rice, and loofah. Verification through examples shows that it can significantly increase crop biomass, tiller number, and final yield in field trials, demonstrating good potential for widespread application. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only one embodiment of the present invention. For those skilled in the art, other embodiments can be derived from the provided drawings without creative effort.

[0021] Figure 1 The protective effect of methyl ferulic acid against apple anthracnose leaf blight pathogen on the leaf surface; Figure 2 Statistical analysis of the number of lesions on apple leaf surface caused by methyl ferulic acid treatment with anthracnose leaf blight. Figure 3 Effects of applying methyl ferulic acid on the aboveground parts of apple seedlings; Figure 4 Effects of applying methyl ferulic acid on the underground parts of apple seedlings; Figure 5 Fresh weight of the above-ground and underground parts of apple seedlings after application of methyl ferulic acid; Figure 6 Height of apple seedlings above and below ground after application of methyl ferulic acid; Figure 7 Expression levels of disease resistance-related genes in apple leaves after methyl ferulic acid treatment; Figure 8 The growth-promoting effect of methyl ferulic acid on pepper seedlings. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. The following embodiments are only for illustrative purposes and are not intended to limit the scope of the present invention in any way. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention. Unless otherwise specified, the experimental methods used in the present invention are conventional methods. Unless otherwise specified, the materials and reagents used in the present invention are commercially available. Furthermore, other terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0023] To ensure a complete and unambiguous understanding of the technical solution of this invention, the term "apple" as used in this invention refers to an apple tree (sapling). Malus domestica (This refers to) tissue culture seedlings obtained through tissue culture, rather than simply edible apple fruits.

[0024] Example 1: The efficacy of methyl ferulic acid against apple anthracnose leaf blight Fresh Gala apple leaves were collected, thoroughly rinsed with sterile water, and then air-dried. Using an in vitro leaf culture method, the petioles were wrapped in moistened absorbent cotton and placed in a sterile, humidified culture box. The experimental group leaves were sprayed evenly with methyl ferulic acid solutions at concentrations of 0.05 μg / mL, 0.1 μg / mL, 0.25 μg / mL, and 0.5 μg / mL, respectively, while the control group was sprayed with an equal volume of sterile water as a blank control. All treatments were cultured under the same humidified conditions for 48 h. The strain of *Anthracnose leaf blight* was cultured on liquid Czapek's medium for 3 days. After filtration through two layers of lens paper to obtain a spore suspension, the spores were collected by centrifugation at 5000 rpm for 10 minutes, rinsed with sterile water, and the spore concentration was adjusted to 1×10⁻⁶. 6 The concentration of lesions was increased to 0.1% Tween 20, and the solution was sprayed evenly onto the detached fresh leaves. The leaves were kept moist, and the number of lesions was counted and photographed after 4-6 days. The methyl ferulic acid used was a 95% purity technical grade, purchased from Maclean's Reagents.

[0025] Experimental results are as follows Figure 1-2 As shown, the number of lesions on apple leaves treated with different concentrations of methyl ferulic acid decreased significantly with increasing concentration. The water control group (CK) had the most lesions, exceeding 150; while the 0.5 μg / mL treatment group had very few lesions, almost none. This indicates that methyl ferulic acid has a significant inhibitory effect on *Hydrocotyle erythrorhizon*, the causal agent of apple anthracnose leaf blight, and the higher the concentration, the better the control effect, effectively reducing the formation of leaf lesions caused by pathogen infection.

[0026] Therefore, considering both the prevention and control effect and the economic cost, although the 0.1 μg / mL concentration has moderate efficacy, it has a high cost-effectiveness. Therefore, this concentration was used for further research in subsequent experiments.

[0027] Example 2: The effect of methyl ferulic acid on promoting the growth of apple seedlings Six to seven pots of 4-5 leaf-aged apple rootstock seedlings of variety "935" were cultured in a 25℃ climate chamber with a 16:8 light: dark ratio. Foliar spraying and root drenching with a 0.1 μg / mL methyl ferulic acid solution were performed, while a control group was sprayed and drenched with an equal volume of sterile water as a blank control. Application was repeated every 7 days for 60 consecutive days. The roots were photographed, and the weight and height of the above-ground and underground parts were measured.

[0028] Experimental results are as follows Figure 3-6 As shown, Figure 3 Showing the aboveground phenotypes (plant height, leaf area) of the treatment groups and Figure 4 The results showed that the morphology of the underground root system (root length and number of root branches) was significantly better than that of the control group (CK). like Figure 5As shown, further biomass measurements revealed that the aboveground and underground fresh weights of the methyl ferulic acid-treated group were significantly higher than those of the control group, while the aboveground and underground dry weights were also significantly higher. These results indicate that this treatment significantly promotes vegetative growth and effectively enhances biomass accumulation efficiency.

[0029] Example 3: Effects of methyl ferulate on the expression levels of members of the disease-associated pathological gene (PR) family Following the procedure described in Example 1, fresh 'Gala' apple leaves were treated with 0.1 μg / mL methyl ferulic acid solution for 48 h before sampling. The samples were immediately flash-frozen in liquid nitrogen and stored at -80°C for subsequent RNA extraction. Total RNA was extracted from the apple leaves using a plant total RNA extraction kit manufactured by Acrel Biotech Co., Ltd. The integrity and purity of the RNA were assessed by agarose gel electrophoresis and a nucleic acid concentration analyzer. One μg of high-quality total RNA was used to synthesize first-strand cDNA according to the Novizan HiScript® III RT SuperMix reverse transcription kit.

[0030] Using the above cDNA as a template, the QuantStudio® series real-time quantitative PCR system was used to perform the assay on apples. MdEFa1 Using genes as internal controls, the effects of methyl ferulic acid treatment on the expression levels of a series of disease resistance-related pathological genes (PR) family members in apple leaves were systematically analyzed using the SYBR Green qPCR method, including... MdPR1 , MdPR2 , MdPR4 , MdPR5 , MdPR8 and MdPR10 Three technical replicates were set up for each reaction to ensure data reliability.

[0031] Use 2 –ΔΔCT The method involves quantitative analysis of relative gene expression levels using qRT-PCR results. The results are as follows: Figure 7 As shown, compared with the sterile water control group, treatment with 0.1 μg / mL methyl ferulic acid solution significantly induced all assays. PR Gene expression is upregulated. Among them, MdPR1 , MdPR2 As a marker gene for the salicylic acid signaling pathway, its strong activation indicates that systemic acquired resistance (SAR) is effectively triggered; MdPR4 , MdPR8 It belongs to the chitinase class, and its upregulation indicates enhanced cell wall degradation resistance mechanisms; MdPR5 (sweetenedin) and MdPR10Increased expression of (ribonuclease analogs) further enhances the plant's molecular defense against pathogen infection. This suggests that methyl ferulic acid can be used as a novel plant immune inducer.

[0032] Example 4: Preparation process of methyl ferulic acid suspension liquid fertilizer (1) Weighing of pesticide technical: Weigh the mass of each component of the pesticide according to the weight percentage; (2) Sand milling of pesticide components: Add pesticide components and an appropriate amount of water to a sand mill containing zirconia beads for sand milling, so that D90≤5μm, and according to the properties of the pesticide active ingredient, condensate water is passed through to lower the temperature to obtain a pesticide solution; (3) Add wetting agent, dispersant, thickener, defoamer, stabilizer, antifreeze and methyl ferulic acid to the pesticide solution, mix well, and you will get a suspension of liquid fertilizer containing methyl ferulic acid. Example 5: Effects of methyl ferulic acid on the aboveground growth of chili peppers Test crops: chili pepper, Julong Test samples: 2 mg / mL methyl ferulic acid, 0.004% 28-homobrassinolide Experimental design: Chili seeds were surface-sterilized with 1% sodium hypochlorite for 10 minutes, rinsed three times with sterile water, germinated in petri dishes, and then sown. When the chili seedlings reached the 5-leaf stage, seedlings with uniform growth were transplanted into flower pots, one seedling per pot.

[0033] Three treatment groups were set up for the experiment: (1) water control; (2) 2 mg / mL methyl ferulic acid, diluted 2000 times and sprayed; (3) 0.004% 28-homobrassinolide, diluted 2000 times and sprayed. Three pots of pepper seedlings were used for each treatment, and the treatment was carried out twice at 7-day intervals. The results were investigated 14 days after the second treatment. The plant height, number of leaves and chlorophyll content were recorded and counted. Experimental results: The growth-promoting effect of the tested samples on the aboveground parts of chili peppers was as follows: Figure 8 As shown in Table 1, Figure 8 Compound A in the text refers to methyl ferulic acid.

[0034] Depend on Figure 8 As shown in Table 1, both methyl ferulic acid and 28-homobrassinolide can increase plant height, leaf number, and chlorophyll content, thereby promoting the growth of peppers, and their growth-promoting effects are comparable.

[0035] Table 1:

[0036] Example 6 Field trial of 40% tebuconazole·oximeprobamate suspension fertilizer containing methyl ferulic acid. Experimental crop: Rice, Jinjing 565 Test reagents: 40% tebuconazole·oximetrimer suspension (Qingdao Zhongda Agricultural Technology Co., Ltd.); 0.004% 28-homobrassinolide soluble concentrate (Jiangxi Weidi Biotechnology Co., Ltd.); 40% tebuconazole·oximetrimer suspension liquid fertilizer containing methyl ferulic acid (self-made, 30% tebuconazole, 10% oximetrimer, 0.2% methyl ferulic acid, 5% naphthalenesulfonate formaldehyde condensate, 0.5% organosilicone oil, 1% magnesium aluminum silicate, 0.1% xanthan gum, 0.2% sodium benzoate, 4% ethylene glycol, water to make up).

[0037] Test location: Group 1, Suya Village, Xiaji Town, Baoying County, Yangzhou City, Jiangsu Province Experimental Design: The experiment consisted of four treatments, with identical management methods applied to each treatment area. Backpack sprayers were used, and each treatment area was 30m². 2 The study employed a randomized block design with three replicates. The pesticide was applied on August 1st, and rice growth and disease status were assessed before application and 14 days after application.

[0038] Treatment 1: 40% tebuconazole·oxime promethazine suspension (30 g / acre) Treatment 2: 40% tebuconazole·oxime-based fertilizer containing methyl ferulic acid (30 g / mu) Treatment 3: 0.004% 28-hobrassinolide soluble concentrate (1500 times) + 40% tebuconazole·oxime-based fertilizer (30 g / mu) Treatment 4: Clean water Experimental surveys and statistical methods During the survey, samples were taken from five points along the diagonal of each plot, with five adjacent clumps at each point, for a total of 25 clumps. The plant height, number of tillers, number of diseased plants and disease severity, and thousand-grain weight at harvest were recorded.

[0039] The disease grading criteria are as follows: Grade 0: The entire plant is disease-free; Grade 1: Disease occurs on the fourth leaf and all leaf sheaths and leaves below it (with the sword leaf being the first leaf). Grade 3: Disease occurs on the third leaf and all leaf sheaths and leaves below it; Level 5: Disease occurs on the second leaf and all leaf sheaths and leaves below it; Level 7: Disease occurs on the sword-shaped leaf blade and all leaf sheaths and leaves below it; Level 9: The entire plant is infected and dies prematurely.

[0040] The grading standard refers to GB / T17980.20-2000, "Guidelines for Field Efficacy Trials of Pesticides," and is graded according to the severity of disease. Each grade is expressed by a simple numerical value in order of severity, and then calculated using the following formula based on the recorded disease incidence: Disease index = Σ(number of diseased leaves at each level × relative grade value) / (total number of leaves surveyed × 9) × 100 Prevention and control effect = ((disease index in control area - disease index in treatment area) / disease index in control area) × 100 As shown in Table 2, the performance of each treatment before the second application was comparable in terms of plant height, tiller number, and disease index. Fourteen days after the second application, the trends in plant height and tiller number were consistent across the different treatments, with treatments 2 and 3 showing comparable effects and significantly improving rice growth and tillering. In terms of controlling rice sheath blight, treatment 2 showed the best effect, with a control efficacy of 90.17%, slightly higher than treatment 3 and significantly higher than treatment 1, indicating that both methyl ferulic acid and 28-homobrassinolide have the effect of inducing disease resistance. At harvest, treatment 4 had the lowest thousand-grain weight, only 20.3g, while treatment 1 was more effective against sheath blight, with a thousand-grain weight significantly higher than treatment 4, reaching 25.6g. Treatments 2 and 3 were pesticide-fertilizer regimens, which not only promoted rice growth but also had a high control efficacy against sheath blight, with a thousand-grain weight significantly higher than other treatments, ranging from 30.5g to 31.2g, an increase of 50.2% to 55.2% compared to the control.

[0041] The experimental results showed that methyl ferulic acid had a similar effect on promoting rice growth and tillering as 28-homobrassinolide, and both could induce disease resistance in rice. In the control of rice sheath blight, it had a synergistic effect with tebuconazole·oximetronidazole.

[0042] Table 2

[0043] Example 7: Field trial of 8% abamectin·fluopyram suspension containing methyl ferulate as a fertilizer Experimental crops: loofah, Emerald King Test reagents: 8% abamectin·fluopyram suspension (Qingdao Zhongda Agricultural Technology Co., Ltd.); 5% amino oligosaccharide soluble concentrate (Qingdao Zhongda Agricultural Technology Co., Ltd.); 8% abamectin·fluopyram suspension liquid fertilizer containing methyl ferulic acid (self-made, abamectin 2%, fluopyram 6%, compound A 0.2%, alkyl phosphate 2%, alkyl phosphate 2%, polydimethylsiloxane 0.5%, magnesium aluminum silicate 1%, sodium carboxymethyl cellulose 0.1%, sodium benzoate 0.2%, ethylene glycol 4%, water to make up).

[0044] Test location: Lijiazhuang, Shouguang City, Shandong Province Experimental Design: Transplanting was carried out on March 17, 2025, using raised bed planting in a vegetable greenhouse at a density of 2800 plants per 667m². 2The experimental site was managed using standard field practices. The selected greenhouses had a history of severe root-knot nematode infestations.

[0045] The experiment consisted of four treatments, with identical management methods applied to each treatment area. Backpack sprayers were used, and each treatment area was 30m². 2 The study employed a triple-replicated, randomized block design, applying the medication twice, once on March 24th (7 days after transplanting) and again on March 31st (14 days after transplanting), with dosages as shown below. A survey was conducted 20 days after the second application, using a diagonal 5-point sampling method, with at least two plants surveyed at each point, to analyze the growth and disease status of the loofah gourds.

[0046] Treatment 1: 8% abamectin·fluopyram suspension (500 g / mu); Treatment 2: 8% abamectin·fluopyram suspension fertilizer containing compound A (500 g / mu); Treatment 3: 5% amino oligosaccharide soluble concentrate (100 mL / mu) + 8% abamectin·fluopyram suspension (500 g / mu); Treatment 4: Clean water.

[0047] Luffa root-knot nematode disease grading criteria: Level 0: No root knots in the root system; Level 1: 0%-20% of the root system has root knots; Level 3: 21%-40% of the root system has root knots; Level 5: 41%-60% of the root system has root knots; Level 7: 61%-80% of the root system has root knots; Level 9: Over 80% of the root system has root knots; The grading standard refers to GB / T17980.38-2000, "Guidelines for Field Efficacy Trials of Pesticides," and is graded according to the severity of disease. Each grade is expressed by a simple numerical value in order of severity, and then calculated using the following formula based on the recorded disease incidence: Disease index = 100 × (∑(number of plants at each disease level × disease level value)) / (total survey value × 9) Prevention and control efficacy (%) = 100% × (Disease index in blank control area - Disease index in treatment area) / Disease index in blank control area Table 3. Field trial results of 8% abamectin·fluopyram suspension containing compound A

[0048] As shown in Table 3, 20 days after the second application, the trends in plant height, stem diameter, and chlorophyll content were consistent across different treatments for loofah growth indicators. Treatment 2 showed the best effect, followed by treatment 3, but all were higher than treatments 1 and 4, indicating that both compound A and amino oligosaccharide could improve loofah growth, with compound A showing the most significant effect. Regarding the control of root-knot nematodes in loofah, treatment 2 showed the best effect with a control efficacy of 89.71%, followed by treatment 3 at 85.52%, while treatment 1 showed the worst effect with a control efficacy of only 80.96%, indicating that compound A had a better disease-inducing effect than amino oligosaccharide.

[0049] The experimental results showed that compound A was more effective than amino oligosaccharide in improving the growth of loofah and inducing disease resistance, and had a synergistic effect with abamectin-fluopyram in controlling root-knot nematodes in loofah.

[0050] It is understood that those skilled in the art can make equivalent substitutions or modifications to the technical solutions and concepts of this invention, and all such substitutions or modifications should fall within the protection scope of the appended claims.

Claims

1. A compound with inducing resistance and promoting growth, characterized in that, The main active ingredient of the compound is methyl ferulic acid, with an effective concentration of 0.05 μg / mL to 0.5 μg / mL, used to induce plant disease resistance and promote plant growth.

2. A fertilizer with induced resistance and growth-promoting effects, characterized in that, The fertilizer-pesticide mixture includes pesticide active ingredients, methyl ferulic acid, wetting agents, dispersants, thickeners, defoamers, stabilizers, and antifreeze agents; the effective concentration of methyl ferulic acid is 0.05 μg / mL to 0.5 μg / mL.

3. The fertilizer with growth-promoting and resistance-inducing effects according to claim 2, characterized in that, The concentration of methyl ferulic acid is 0.1 μg / mL.

4. The fertilizer with growth-promoting and resistance-inducing effects according to claim 2, characterized in that, The active ingredients of the pesticide include tebuconazole, azoxystrobin, avermectin, fluopyram, or combinations thereof.

5. A method for preparing a medicated fertilizer with induced resistance and growth-promoting effects, characterized in that, Includes the following steps: S1: Weigh the mass of each component of the pesticide according to its weight percentage; S2: Add pesticide components and an appropriate amount of water to a sand mill containing zirconia beads for sand milling until D90≤5μm. According to the properties of the pesticide active ingredient, condensate water is passed through to lower the temperature to obtain a pesticide solution. S3: Add wetting agent, dispersant, thickener, defoamer, stabilizer, antifreeze and methyl ferulic acid to the pesticide solution and mix well.

6. The application of the fertilizer with growth-promoting and resistance-inducing effects as described in any one of claims 2-4, or the fertilizer prepared by the method of claim 5, in promoting plant growth.

7. The application according to claim 6, characterized in that, The plants mentioned include fruit trees, vegetables, and grain and oil crops.

8. The application of the fertilizer with the inducing resistance and promoting growth effect as described in any one of claims 2-4 or the fertilizer prepared by the method of claim 5 in the prevention and control of rice sheath blight.

9. The application of the fertilizer with the inducing resistance and promoting growth effect as described in any one of claims 2-4, or the fertilizer prepared by the method of claim 5, in the control of root-knot nematodes.

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