A kind of oil production method of immobilized culture of microalgae

An immobilized culture, microalgae technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of exchanging the illumination frequency of algal cells, cannot directly use microalgae to immobilize oil production, and is difficult to induce oil. and other problems to achieve the effect of filling the gap

Active Publication Date: 2018-01-02
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional liquid microalgae oil production method cannot be directly applied to immobilized microalgae for oil production.
Because microalgae are immobilized, the cells are highly dense so that the contact between the cells and the culture medium is reduced, and the cells cannot move with the water flow to exchange the light frequency of the algae cells. Grease induction
[0004] In the prior art of immobilized culture of microalgae, there is no method for producing oil under immobilized culture of microalgae

Method used

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  • A kind of oil production method of immobilized culture of microalgae

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Experimental program
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Effect test

specific Embodiment 1

[0075] Evenly smear Scenedesmus species on the immobilized carrier, use full nutrient medium (BG11 liquid medium) to supplement water and nutrition, and pass through the culture medium containing 1% CO 2 of compressed air. During the experiment, the outdoor light intensity at noon was 1500μmol / m 2 / s, the light intensity in the morning and evening is about 300μmol / m 2 / s, the average light intensity during the day is 500μmol / m 2 / s, the average temperature is 25°C. Continuous culture for 7 days, at this time the cell concentration reached 110g / m 2 During the detection period, the chlorophyll fluorescence value (Fv / Fm) of each layer of cells on the growth carrier was stable at about 0.6 on average. Then change the concentration of sodium nitrate in the BG11 medium to 0.5g / L, and induce oil production for 5 days. During the process of inducing oil production, continue sampling to detect the chlorophyll fluorescence value of the microalgae cells on the growth carrier, wherein...

specific Embodiment 2

[0076] Evenly smear Scenedesmus species on the immobilized growth carrier, use full nutrient medium (BG11 liquid medium) to supplement water and nutrients, and pass through the culture medium containing 2% CO 2 of compressed air. During the experiment, the outdoor light intensity at noon was 1500μmol / m 2 / s, the light intensity in the morning and evening is about 300μmol / m 2 / s, the average light intensity during the day is 500μmol / m 2 / s, the average temperature is 25°C. Continuous culture for 4 days, at this time the cell concentration reached 60g / m 2, during which the photosynthetic oxygen evolution of the growth carrier algae cells was detected. Then change the concentration of sodium nitrate in the BG11 medium to 0.3g / L, and induce oil production for 5 days. Algae cell layer cells (the cell layer with almost no oxygen evolution and close to the carrier is classified as the regrowth microalgae cell layer, and the layer that detects a large amount of oxygen and is far ...

specific Embodiment 3

[0077] Evenly smear the chlorella species on the immobilized growth carrier, use the full nutrient medium (BG11 liquid medium) to supplement water and nutrients, and pass through the culture medium containing 2.5% CO 2 of compressed air. During the experiment, the outdoor light intensity at noon was 1500μmol / m 2 / s, the light intensity in the morning and evening is about 300μmol / m 2 / s, the average light intensity during the day is 500μmol / m 2 / s, the average temperature is 25°C. Continuous culture for 4 days, at this time the cell concentration reached 66g / m 2 During the detection period, the chlorophyll fluorescence value (Fv / Fm) of each layer of cells on the growth carrier was stable at about 0.6 on average. Then change the concentration of sodium nitrate in the BG11 medium to 0.4g / L, induce oil production and culture for 4 days, during the process of inducing oil production culture, take samples to detect the chlorophyll fluorescence value on the growth carrier, wherei...

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Abstract

The invention provides a method for producing oil by immobilized culture of microalgae. The method comprises the following steps: (1) inoculating microalgae cells on an immobilized carrier, and cultivating them with a complete nutrient medium; (2) detecting microalgae algae cell concentration, and compare it with the preset microalgae cell concentration; (3) when the actual microalgae cell concentration ≥ the preset microalgae cell concentration, the full nutrient medium is adjusted to nitrogen deficiency culture medium for inducing oleaginous production; (4) after the oleaginous inducing culture is completed, the cell layer of oleaginous microalgae is collected. In the present invention, microalgae cells are inoculated on immobilized carriers, cultured with a full nutrient medium, so that the algae can quickly accumulate biomass, and by detecting the cell concentration and comparing it with the preset cell concentration, the adjusted value of the medium is determined. When the time is right, culture to induce oil production is carried out to realize oil accumulation. After the induction of oil production, the cell layer of oleaginous microalgae whose oil content meets the requirements is collected to realize the oil production of the immobilized culture of microalgae.

Description

technical field [0001] The invention relates to an oil production method for immobilized culture of microalgae, belonging to the technical field of microalgae culture. Background technique [0002] Microalgae use carbon dioxide and water to fix carbon dioxide through photosynthesis and convert solar energy into biomass energy. In the logarithmic growth phase or optimal growth conditions, fixed carbon dioxide is used for the growth and reproduction of algal cells. The algal cells at this stage mainly contain proteins and sugars, and the oil content is very low; Under adverse conditions of physical or chemical stimuli, algal cells will accumulate a large amount of oil to resist adverse conditions. [0003] Microalgae cells grow under immobilized conditions, abandoning the liquid environment of a large amount of water body, making the environment of the whole culture process easier to control, improving the culture efficiency and reducing the culture cost, so that the algae ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/89
Inventor 陈传红陈昱罗少敬李青尹顺吉张惠敏吴洪
Owner ENN SCI & TECH DEV
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