Method used for preparing grease via microalgae mixed cultivation

A technology of mixed culture and microalgae, which is applied in the field of biotechnology and bioenergy, can solve the problems of low efficiency, achieve high carbon fixation efficiency, increase the content of biomass and oil, and improve the effect of fixation efficiency

Active Publication Date: 2016-06-08
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For existing algal species that cannot tolerate and absorb high concentrations of CO 2 , fixed CO 2 In order to solve the problem of low efficiency, the present invention provides a 2 And the method for preparing oil by mixed culture of microalgae under alkaline environment

Method used

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  • Method used for preparing grease via microalgae mixed cultivation
  • Method used for preparing grease via microalgae mixed cultivation
  • Method used for preparing grease via microalgae mixed cultivation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation of embodiment 1 microalgae seed liquid

[0018] BG11 medium was used for microalgae culture, and the medium formulations are shown in Table 1 and Table 2.

[0019] Table 1BG11 medium

[0020]

[0021] * Composition of A5+Cosolution in Table 2 and Table 1

[0022]

[0023] First prepare the BG11 liquid medium according to Table 1 and Table 2, adjust the pH of the medium for cultivating Scenedesmus obliquus FSH-Y2 to 10, adjust the pH of the medium for cultivating Cellulose SS-B7 to 7.5, and then adjust the pH of the medium for cultivating Scenedesmus obliques SS-B7 Scenedesmus FSH-Y2 and cellulosus SS-B7 were inoculated in the above medium respectively. Cultivate in a constant temperature light shaker, the culture temperature is 25°C, the light cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 5000Lux, 120rpm shaking culture to the logarithmic growth phase, and Scenedesmus obliquus FSH-Y2 is obtained The seed liquid and the...

Embodiment 2

[0024] The preparation of embodiment 2 microalgae oil

[0025] (1) In the photobioreactor, add the FSH-Y2 seed solution prepared in Example 1 and the microalgae medium, the volume ratio of the FSH-Y2 seed solution to the medium is 1:5, and the medium is BG11 medium, The pH value of the culture medium was controlled at 10, the light intensity of the culture was 5000Lux, the light cycle was 24h, the light-dark time ratio was 14:10, and a mixed gas of nitrogen and carbon dioxide was introduced, and the carbon dioxide content was 5v%.

[0026](2) After 2 days of cultivation, insert the seed solution of cellulosus SS-B7 prepared in Example 1, the volume ratio of the seed solution to the medium is 1:20, the pH of the medium is controlled at 8, and a mixture of nitrogen and carbon dioxide is introduced gas, of which CO 2 The content is 30v%.

[0027] (3) After cultivating for 7 days, it enters a stable period, ends the culturing, harvests the microalgae cells by centrifugation, and...

Embodiment 3

[0028] The preparation of embodiment 3 microalgae oil

[0029] (1) In the photobioreactor, add the FSH-Y2 seed solution prepared in Example 1 and the microalgae medium, the volume ratio of the FSH-Y2 seed solution to the medium is 1:10, and the medium is BG11 medium, The pH value of the culture medium was controlled at 12, the light intensity of the culture was 5000Lux, the light cycle was 24h, the light-dark time ratio was 14:10, and a mixed gas of nitrogen and carbon dioxide was introduced, and the carbon dioxide content was 5v%.

[0030] (2) After culturing for 3 days, insert the seed solution of Cellulosa SS-B7 prepared in Example 1, the volume ratio of the seed solution to the medium is 1:20, the pH of the medium is controlled at 10, and a mixture of nitrogen and carbon dioxide is introduced gas, of which CO 2 The content is 25v%.

[0031] (3) After cultivating for 7 days, enter the stable period, end the culturing, harvest the microalgae cells by centrifugation, measur...

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Abstract

The invention discloses a method used for preparing grease via microalgae mixed cultivation. The method comprises following steps: 1, a microalgae culture medium and a Scenedesmus obliqnus FSH-Y2 seed solution are delivered into a photobioreactor, pH value of an obtained culture system is adjusted to be 10 to 12, CO2 content of added gas is controlled to be lower than 5v%, and culturing is carried out for 2 to 5 days; 2, pH value of the culture system is adjusted to be 8 to 10, an Ankistrodesmus sp. SS-B7 seed solution is added for mixed cultivation, and CO2 content of added gas is controlled to be 5 to 45v%; 3, culturing is carried out until stationary growth stage is over, and microalgae cells are harvested. The Scenedesmus obliqnus FSH-Y2 and the Ankistrodesmus sp. SS-B7 are stored in China General Microbiological Culture Collection Center on 11th September 2012 and 15th April 2013 respectively, and preservation number is CGMCC No. 6551 and CGMCC No.7478. The method is capable of increasing tolerance of the microalgae culture system on CO2, and solubleness, and increasing carbon sequestration efficiency; microalgae grease yield is increased obviously; and the method can be used for biodiesel production.

Description

technical field [0001] The invention belongs to the field of biotechnology and bioenergy, in particular to a 2 And the method for preparing oil by mixed culture of microalgae under alkaline environment. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/64C12R1/89
CPCY02E50/10
Inventor 师文静廖莎姚新武樊亚超孙启梅王领民高大成李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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