25 results about "Ethanol synthesis" patented technology

The invention discloses an application of some functional genes in improving the performance of clostridium for synthesizing ethanol by utilizing one-carbon gas fermentation, wherein overexpression genes NCBI (National Center for Biotechnology Information) are CLJUc04490, CLJUc25060, CLJUc06990, CLJUc22650 and / or CLJUc07720, and expression of inhibition genes CLJUc09160 and / or CLJUc23840 can be used for improving the autotrophic growth capability and the ethanol synthesis yield of clostridium Yangdall when the clostridium Yangdall is fermented by utilizing C1 gas; therefore, the economical efficiency of the clostridium yangdall fermentation is improved.

Escherichia coli is modified by means of metabolic engineering, a genetically engineered bacterium for high yield of O-succinyl-L-homoserine is obtained, and a construction method of the genetically engineered bacterium comprises the following steps: weakening a synthetic route of an organic acid metabolic byproduct of a chassis strain E.coli W3110 [delta] metI [delta] metJ [delta] thrB [delta] metB; wherein the synthetic route of the organic acid metabolic byproducts is selected from at least one of a formic acid synthetic route, a lactic acid synthetic route and an ethanol synthetic route. The constructed genetically engineered bacterium is applied to microbial fermentation production of the O-succinyl-L-homoserine, effective accumulation of the O-succinyl-L-homoserine is achieved, the sugar acid conversion rate reaches 46.8%, and a foundation is laid for subsequent construction of high-yield O-succinyl-L-homoserine engineering bacteria.

The invention discloses a method for improving the performance of clostridium aerovorans for synthesizing ethanol by utilizing gas fermentation. The method comprises the following steps: enabling the clostridium aerovorans to over-express ethanol synthesis pathway genes ald, adhE1 and adhE2; the expression of genes CLJU23220, CLJU25810 and CLJU25840 in the synthetic route of the 2, 3-butanediol is inhibited; and overexpressing acetic acid reuse genes aor1 and aor2. According to the clostridium yangdall engineering bacteria constructed by the method, the yield of ethanol synthesized by fermentation of synthesis gas is increased by 700% or above and reaches 1.70 g / L or above, and the synthesis amount of a by-product acetic acid is reduced by 18%.

The invention discloses a method for improving the fermentation performance of clostridium aerovorans and application of the clostridium aerovorans, and according to the method, an effective amount of methionine or a derivative thereof is added into a fermentation culture medium of the clostridium aerovorans to completely replace expensive complex organic nitrogen sources such as yeast extracts. The method successfully solves the three technical bottlenecks of slow biomass accumulation, low ethanol yield and dependence on expensive nitrogen sources in the fermentation process of the clostridium aerovorans by utilizing synthesis gas or CO2 / H2 mixed gas. Experiments prove that methionine or a derivative thereof can synergistically promote growth metabolism of thalli and a synthesis path of a product, so that the gas conversion efficiency of a carbon source is remarkably improved and metabolism is guided to flow to ethanol synthesis while the growth rate and the final biomass of the thalli are improved, and thus the production capacity of alcohols is synchronously enhanced. The method is simple in process and remarkable in effect, and an efficient and economical new strategy is provided for reducing the production cost of biofuel and promoting industrial application of a synthesis gas biorefinery technology.

The invention relates to the technical field of alcohol synthesis catalysts, in particular to a doped Cu-based catalyst, a preparation method and application of the doped Cu-based catalyst in the aspect of ethanol synthesis. The preparation method comprises the following steps: S1, pretreating mesoporous silica; s2, loading zinc ions on the pretreated mesoporous silica; s3, converting the zinc ions loaded on the mesoporous silica into zinc oxide; s4, treating the mesoporous silica adsorbed with zinc oxide through EDTA (Ethylene Diamine Tetraacetic Acid); s5, performing fixed-point adsorption on the copper ions by zinc oxide on the mesoporous silica; s6, mesoporous silica is removed through alkali treatment, and the doped Cu-based catalyst is prepared through heating reduction. According to the doped Cu-based catalyst prepared by the invention, copper ions and zinc oxide are uniformly distributed, so that the catalytic efficiency of the material on synthesis gas is effectively improved.

This invention discloses a method for the synthesis of furfural and ethanol from green coconut (Cocos nucifera L.) husk. The method involves chemical reaction for furfural production and a biological fermentation for ethanol synthesis. In the chemical step, the green coconut husk undergoes acid hydrolysis in a high-pressure reactor, yielding furfural. The residual waste from this step is then utilized as a substrate for fermentation by Saccharomyces cerevisiae, producing ethanol. The integrated approach efficiently converts the cellulosic and hemicellulosic components of the green coconut husk into two industrially valuable compounds, furfural and ethanol, from a single agricultural waste stream. The invention provides a cost-effective and environmentally sustainable method for resource utilization and waste reduction.

The invention discloses short-chain dehydrogenase for synthesis of (S)-1-(2-methoxy-3-bromophenyl) ethanol as well as a mutant, a coding gene, a plasmid, a genetically engineered bacterium and application of the short-chain dehydrogenase, and belongs to the technical field of biocatalysts. The mutant is obtained by mutating methionine at the 97th site into isoleucine on the basis of a wild type enzyme shown in SEQ ID NO.1 (SEQ ID NO.3). The invention further discloses a preparation method of the short-chain dehydrogenase. The invention also provides a coding gene, an expression vector, a genetically engineered bacterium and a double-enzyme catalytic system containing the mutant and formate dehydrogenase. The system can efficiently and highly selectively catalyze and synthesize the target chiral alcohol under mild conditions, and the conversion rate is gt; 95%, eet; 99% of the method is suitable for green preparation of the key intermediate of the medicine lutrombopag.

The invention discloses a pyruvic acid gene loop mediated saccharomyces cerevisiae ethanol metabolism redirection method. Comprising the following steps: constructing a pyruvic acid response gene loop in saccharomyces cerevisiae host bacteria to obtain modified saccharomyces cerevisiae; the growth of saccharomyces cerevisiae cells, the synthesis of by-product ethanol and the synthesis of products are dynamically regulated and modified by controlling glucose feeding. A low-concentration glucose environment is maintained in the early stage of growth, so that cell growth and ethanol metabolism related genes are normally expressed, and cell growth is recovered; high-concentration glucose is supplemented in the later stage of fermentation, expression of cell growth related ethanol metabolism genes is inhibited, the ethanol synthesis flux is reduced, sufficient pyruvic acid precursors are provided for product synthesis, and the product synthesis flux is maximized. According to the method, the yield of the byproduct ethanol is reduced, the production efficiency of the product is improved, and meanwhile, the fermentation period is shortened.

The application discloses a Cu / ZrO2@NC catalyst for catalyzing synthesis of high-carbon alcohol, a preparation method and application thereof, and relates to the technical field of high-carbon alcohol compound synthesis. The catalyst is prepared by adding copper salt and zirconium salt and polyacrylamide into water to dissolve and mix, heating and stirring to form a complex homogeneous solution, and drying to obtain a copper-zirconium catalyst precursor. The copper-zirconium catalyst precursor is calcined under an inert gas atmosphere to obtain the Cu / ZrO2@NC catalyst. The Cu / ZrO2@NC catalyst prepared by the application has the characteristics of high loading, low particle size and high directional reaction activity for high-carbon alcohol. The catalyst can effectively realize the effective coupling of aldehyde-ketone condensation and hydroxy-aldehyde condensation for synthesis of high-carbon alcohol from ethanol, and can obtain an oil phase product mainly composed of C6+ high-carbon alcohol with high selectivity.

The present invention provides a metal catalyst and a process for catalytic conversion of ethanol. The present invention provides the synthesis of highly valuable ethyl acetate from ethanol, employing a plethora of group (VIII) metal complexes. The present invention provides generation of green hydrogen gas as a source of clean energy and ethyl acetate as an important industrial substrate from ethanol, catalyzed by a range of group (VIII) metal complexes based on a variety of ligands. Herein, the low-cost ethanol is converted to industrially valuable ethyl acetate having numerous applications in paints and pharmaceutical industry.

The invention relates to a saccharomyces cerevisiae strain for producing 2, 3-butanediol through bidirectional light regulation and control as well as a construction method and application of the saccharomyces cerevisiae strain. The Saccharomyces cerevisiae strain for producing 2, 3-butanediol through bidirectional light regulation and control is named as BYSDBSPP, contains a PSD and SULI double-light-control system, and respectively regulates and controls an ethanol synthesis route and a 2, 3-butanediol synthesis route. The method is used for producing 2, 3-butanediol in a lysine-deficient SD liquid culture medium under the condition of light stimulation; the wavelength range of the light stimulation is 400 to 500 nm. According to the invention, the problem of bacterial strain growth defects caused by gene knockout is avoided, and the production of various target metabolites taking ethanol as a byproduct is facilitated. The light regulation system for controlling the protein level is adopted, the stability of specific zymoprotein in a metabolic pathway can be directly controlled from the post-translational level, and in metabolic engineering application, compared with a light regulation system for controlling the gene transcription level, the response speed is higher, and strain modification application is simpler and more convenient.

The present application relates to the technical field of bioengineering, and particularly relates to a Kluyveromyces marxianus strain and application thereof in fermentation production of ethanol. The present application provides a Kluyveromyces marxianus strain huJ01, and the preservation number of the strain is CGMCC No.22931. The strain can still maintain good growth and ethanol synthesis capacity under stress conditions such as high temperature, high acid, high ethanol and high salt. Under the optimal conditions of inulinase activity, the ethanol yield can reach 2.41%vol after 20% inoculation and static culture at 60 DEG C for 120 h. The strain is inoculated at 4% in a high stress culture medium containing 11.4% ethanol, pH 3.5 and 3.51% NaCl, and is statically cultured at 40 DEG C for 12 h, and the concentration of the bacterial liquid is significantly increased or not significantly decreased. It is shown that the strain still has strong ethanol fermentation performance under extreme environment, and is suitable for special industrial fermentation scenes such as high salt, high acid and high ethanol.

The present application relates to the technical field of microbial fermentation, and particularly relates to a method for producing ethanol and / or single-cell protein by Clostridium fermentation of synthesis gas. The present application significantly improves the biomass and the yield of ethanol by supplementing nitrogen source and sulfur source and optimizing the supplementing mode during the process of ethanol production by Clostridium fermentation of synthesis gas, so that ethanol becomes the main product of fermentation. The fermentation production method provided by the present application has the advantages of high efficiency, high yield, high proportion of ethanol in fermentation products, high biomass, simple process and easy operation, and has a good application prospect in the production of ethanol by synthesis gas fermentation, and provides a new means and method for efficient synthesis of ethanol and single-cell protein by synthesis gas.

The invention belongs to the technical field of catalysts. The invention provides quaternary ammonium base ionic liquid with a structure as shown in a formula (I). The method can be used for synthesis processes of synthesizing methyl ethyl carbonate from dimethyl carbonate and diethyl carbonate and synthesizing methyl ethyl carbonate from dimethyl carbonate and ethanol, and the synthesized methyl ethyl carbonate has excellent purity. Besides, the used quaternary ammonium base ionic liquid has the advantages of being easy to prepare, low in toxicity and renewable, the problems that a traditional sodium alcoholate catalyst is easy to cake and inactivate and difficult to reuse are solved, and the method has important significance for promoting green and efficient production of methyl ethyl carbonate.

The invention belongs to the technical field of ethanol synthesis, and discloses a gas-liquid combined two-step method acetic acid hydrogenation device and process. The device comprises a heating mixer, a reactor, a gas-liquid separator, a liquid phase hydrogenation reaction kettle, a separator and a rectifying tower which are connected in sequence. The process comprises three working sections of acetic acid hydrogenation, acetaldehyde and acetic ether hydrogenation and crude product purification. Acetic acid is hydrogenated under the action of a catalyst to prepare acetaldehyde and acetic ether, in the acetaldehyde and acetic ether hydrogenation section, a reaction mixture in the acetic acid hydrogenation section is directly hydrogenated to prepare ethanol, hydrogen-rich tail gas and fresh hydrogen are mixed and enter the acetic acid hydrogenation section to serve as reaction gas, crude ethanol is rectified and purified, and high-purity ethanol is obtained. According to the invention, continuous production is realized, the hydrogen is subjected to deep reaction, the raw materials have higher conversion rate and yield, and the operation continuity and stability are very high. The corrosion of direct hydrogenation of acetic acid to equipment is reduced, and the defects of high energy consumption and huge equipment scale caused by an ethanol system are avoided.

The present invention relates to the technical field of alcohol synthesis catalysts, specifically a doped Cu-based catalyst, a preparation method, and its application in ethanol synthesis. The preparation method comprises the following steps: S1, pretreating mesoporous silica; S2, loading zinc ions onto the pretreated mesoporous silica; S3, converting the zinc ions loaded on the loaded mesoporous silica into zinc oxide; S4, treating the mesoporous silica adsorbed with zinc oxide with EDTA; S5, subjecting the zinc oxide on the mesoporous silica to point-specific adsorption of copper ions; S6, removing the mesoporous silica through alkaline treatment, and heating and reducing the catalyst to obtain a doped Cu-based catalyst. The doped Cu-based catalyst prepared by the present invention has uniform distribution of copper ions and zinc oxide, effectively improving the material's catalytic efficiency for synthesis gas.

The invention belongs to the technical field of microorganisms, and discloses a Saccharomyces cerevisiae DG-11 strain with low acetic acid yield, and the preservation number of the Saccharomyces cerevisiae DG-11 strain is CCTCC (China Center for Type Culture Collection) M 20251925. The strain is obtained by combining ARTP mutagenesis with 2-deoxyglucose (2-DG) resistance screening, and has the following characteristics that in simulated fruit juice fermentation, the yield of acetic acid is remarkably reduced compared with that of an original strain RV002; in the metabolic pathway, the activities of pyruvate decarboxylase (Pdc) and acetaldehyde dehydrogenase (Ald) are inhibited, and the activity of ethanol dehydrogenase (Adh) is enhanced, so that carbon metabolism is inclined to ethanol synthesis; meanwhile, the strain has excellent ethanol, high glucose and pH tolerance, and is stable in hereditary character. In addition, the content of acetic esters in the DG-11 fermentation liquor is reduced, and the content of part of ethyl esters and higher alcohols is increased, so that the flower and fruit aroma characteristics are favorably formed. The saccharomyces cerevisiae strain with excellent performance and low acetic acid yield is provided for fruit wine brewing, and the saccharomyces cerevisiae strain has important industrial application value.

The utility model discloses a green electricity coupling coal salt comprehensive utilization device. Feed coal is connected to the input end of the gasification furnace, and the output end of the gasification furnace is connected to the input end of the washing device. The raw material salt is connected to the first input end of the electrolysis device; a first output end, a second output end and a third output end of the methanol synthesis device are respectively connected to a second input end of the ethanol synthesis device, a second input end of the acetic acid synthesis device and the olefin synthesis device; the output end of the ethanol synthesis device is connected to an ethanol product line; a first output end of the olefin synthesis device and an output end of the first polymerization device are respectively connected to a first input end and a second input end of the second polymerization device, and an output end of the second polymerization device is connected to an EVA product line; and the output end of the oxidation synthesis device is connected with an epoxypropane product line. The coal-salt-electricity-chemical-material industrial structure is jointly constructed through coal and salt resources, the resource utilization rate is higher, and carbon emission is greatly reduced.

An electrolyzer system is provided with a plurality of separator-electrode assemblies (SEA). The SEAs include a negative electrode, a positive electrode, and a porous separator positioned therebetween. The electrodes and the separator, and thus the SEA overall, have a flow-through configuration enabling bulk liquid- and gas-phase reactants and products to flow therethrough. SEAs are positioned serially along a length of the electrolyzer, and thus bulk flow occurs through each SEA cell sequentially, in contrast to "flow-by" architectures where flow occurs in parallel through all cells simultaneously. Liquid- and gas-phase inlets provide the reactants such as H2, CO2, O2, etc. which are electrochemically converted to value products by the SEAs and concentrated for removal by product outlets. These electrolyzer systems generate value products such as H2O2; epoxides; C2+ alcohols, such as ethanol; syngas (CO + H2); etc. even at room temperature while avoiding the disadvantages associated with degradation-prone polymeric ion exchange membranes.

The invention discloses a method for strengthening directional synthesis of medium-chain fatty acid from kitchen waste, and belongs to the technical field of resource utilization of organic waste. The method comprises the following steps: pretreating the kitchen waste to prepare slurry with the total solid concentration of 10-20%; then respectively carrying out ethanol synthesis and short-chain fatty acid synthesis to obtain ethanol fermentation liquor and SCFAs fermentation liquor; and finally, centrifugally filtering the ethanol fermentation liquor or the ethanol fermentation liquor and the SCFAs fermentation liquor, mixing to form mixed fermentation liquor, inoculating chain extension sludge, adding 0.1-0.1% (v / v) of chloroform, adding a fermentation culture medium and trace elements, and carrying out anaerobic fermentation to generate medium-chain fatty acid. According to the method, only the kitchen waste slurry is used as a raw material, directional fermentation is realized by accurately regulating and controlling parameters of each stage, an electron donor, an electron acceptor and an auxiliary strengthening technology are not needed, and the production cost is reduced. Competitive microorganisms such as methanogens, acetogens and the like can be synchronously inhibited by adding chloroform, chain extension function microorganisms are not influenced, and the yield and purity of MCFAs are effectively improved.

The catalyst in this embodiment of the invention is a catalyst for the synthesis of 1,3-butadiene from ethanol. This catalyst comprises a porous silica support made of crystalline silica, Zn, and Zr, and has a peak pore size (D) of less than 2 nm. 微 ) and peak aperture greater than 2 nm (D 中+大 The multi-peaked pore size distribution.

A catalyst according to an embodiment is a catalyst for synthesizing 1,3-butadiene from ethanol. The catalyst contains a porous silica support made of crystalline silica, Zn, and Zr, and has a multimodal pore size distribution with a peak pore size (Dmicro) of 2 nm or less and a peak pore size (Dmeso+macro) of more than 2 nm.

This utility model discloses a continuously dehydrated ethanol synthesis reaction system, comprising: a gas-liquid reactor, wherein the lower part of the gas-liquid reactor is provided with a first air inlet for inputting fresh raw materials, and the upper part is provided with a first liquid inlet for inputting fresh raw materials; a first air outlet at the top of the gas-liquid reactor is connected to a second air inlet at the lower end of the gas-liquid reactor via a pipeline to form a gas-phase external circulation loop; a first liquid outlet at the bottom of the gas-liquid reactor is connected to the second liquid inlet at the upper part of the gas-liquid reactor via a dehydration device to form a liquid-phase external circulation loop; wherein the dehydration device includes at least two parallel adsorption columns, which allows the dehydration device to alternately perform water adsorption and water desorption operations. By using at least two parallel adsorption columns to alternately adsorb and desorb, continuous production operation can be achieved, and the adsorption / desorption cycle can be dynamically adjusted according to the reactor water outlet rate to optimize adsorption efficiency and improve reaction efficiency.

This invention provides an ethanol synthesis catalyst for synthesizing ethanol from carbon dioxide, comprising at least one support selected from the group consisting of ZrO2, Al2O3, and TiO2, Fe and Cu supported on said support, and Na and / or K supported on said support. In one embodiment, the catalyst containing iron oxide and copper oxide as Fe and Cu supported on the support can be used. Hydrogen is supplied to the catalyst to reduce the iron oxide and copper oxide, and a reaction gas containing CO2 is supplied to the reduced catalyst to obtain ethanol from CO2.

The invention relates to a composition, production method, or synthesis method for obtaining Tetracycline (Chemical formula C22H24N2O8) from bamboo. The raw materials consist of 1) Bamboo, 2) A substance for reacting with bamboo (water / aqueous agent), and 3) Ethyl Alcohol. The synthesis process involves two main steps: reacting the bamboo with the water-based agent, followed by reacting the resulting substance with Ethyl Alcohol.