16 results about "Ethanol synthesis" patented technology

Escherichia coli is modified by means of metabolic engineering, a genetically engineered bacterium for high yield of O-succinyl-L-homoserine is obtained, and a construction method of the genetically engineered bacterium comprises the following steps: weakening a synthetic route of an organic acid metabolic byproduct of a chassis strain E.coli W3110 [delta] metI [delta] metJ [delta] thrB [delta] metB; wherein the synthetic route of the organic acid metabolic byproducts is selected from at least one of a formic acid synthetic route, a lactic acid synthetic route and an ethanol synthetic route. The constructed genetically engineered bacterium is applied to microbial fermentation production of the O-succinyl-L-homoserine, effective accumulation of the O-succinyl-L-homoserine is achieved, the sugar acid conversion rate reaches 46.8%, and a foundation is laid for subsequent construction of high-yield O-succinyl-L-homoserine engineering bacteria.

The invention discloses a method for improving the performance of clostridium aerovorans for synthesizing ethanol by utilizing gas fermentation. The method comprises the following steps: enabling the clostridium aerovorans to over-express ethanol synthesis pathway genes ald, adhE1 and adhE2; the expression of genes CLJU23220, CLJU25810 and CLJU25840 in the synthetic route of the 2, 3-butanediol is inhibited; and overexpressing acetic acid reuse genes aor1 and aor2. According to the clostridium yangdall engineering bacteria constructed by the method, the yield of ethanol synthesized by fermentation of synthesis gas is increased by 700% or above and reaches 1.70 g / L or above, and the synthesis amount of a by-product acetic acid is reduced by 18%.

The invention discloses a method for improving the fermentation performance of clostridium aerovorans and application of the clostridium aerovorans, and according to the method, an effective amount of methionine or a derivative thereof is added into a fermentation culture medium of the clostridium aerovorans to completely replace expensive complex organic nitrogen sources such as yeast extracts. The method successfully solves the three technical bottlenecks of slow biomass accumulation, low ethanol yield and dependence on expensive nitrogen sources in the fermentation process of the clostridium aerovorans by utilizing synthesis gas or CO2 / H2 mixed gas. Experiments prove that methionine or a derivative thereof can synergistically promote growth metabolism of thalli and a synthesis path of a product, so that the gas conversion efficiency of a carbon source is remarkably improved and metabolism is guided to flow to ethanol synthesis while the growth rate and the final biomass of the thalli are improved, and thus the production capacity of alcohols is synchronously enhanced. The method is simple in process and remarkable in effect, and an efficient and economical new strategy is provided for reducing the production cost of biofuel and promoting industrial application of a synthesis gas biorefinery technology.

This invention discloses a method for the synthesis of furfural and ethanol from green coconut (Cocos nucifera L.) husk. The method involves chemical reaction for furfural production and a biological fermentation for ethanol synthesis. In the chemical step, the green coconut husk undergoes acid hydrolysis in a high-pressure reactor, yielding furfural. The residual waste from this step is then utilized as a substrate for fermentation by Saccharomyces cerevisiae, producing ethanol. The integrated approach efficiently converts the cellulosic and hemicellulosic components of the green coconut husk into two industrially valuable compounds, furfural and ethanol, from a single agricultural waste stream. The invention provides a cost-effective and environmentally sustainable method for resource utilization and waste reduction.

The invention discloses short-chain dehydrogenase for synthesis of (S)-1-(2-methoxy-3-bromophenyl) ethanol as well as a mutant, a coding gene, a plasmid, a genetically engineered bacterium and application of the short-chain dehydrogenase, and belongs to the technical field of biocatalysts. The mutant is obtained by mutating methionine at the 97th site into isoleucine on the basis of a wild type enzyme shown in SEQ ID NO.1 (SEQ ID NO.3). The invention further discloses a preparation method of the short-chain dehydrogenase. The invention also provides a coding gene, an expression vector, a genetically engineered bacterium and a double-enzyme catalytic system containing the mutant and formate dehydrogenase. The system can efficiently and highly selectively catalyze and synthesize the target chiral alcohol under mild conditions, and the conversion rate is gt; 95%, eet; 99% of the method is suitable for green preparation of the key intermediate of the medicine lutrombopag.

The present application relates to the technical field of bioengineering, and particularly relates to a Kluyveromyces marxianus strain and application thereof in fermentation production of ethanol. The present application provides a Kluyveromyces marxianus strain huJ01, and the preservation number of the strain is CGMCC No.22931. The strain can still maintain good growth and ethanol synthesis capacity under stress conditions such as high temperature, high acid, high ethanol and high salt. Under the optimal conditions of inulinase activity, the ethanol yield can reach 2.41%vol after 20% inoculation and static culture at 60 DEG C for 120 h. The strain is inoculated at 4% in a high stress culture medium containing 11.4% ethanol, pH 3.5 and 3.51% NaCl, and is statically cultured at 40 DEG C for 12 h, and the concentration of the bacterial liquid is significantly increased or not significantly decreased. It is shown that the strain still has strong ethanol fermentation performance under extreme environment, and is suitable for special industrial fermentation scenes such as high salt, high acid and high ethanol.

The present application relates to the technical field of microbial fermentation, and particularly relates to a method for producing ethanol and / or single-cell protein by Clostridium fermentation of synthesis gas. The present application significantly improves the biomass and the yield of ethanol by supplementing nitrogen source and sulfur source and optimizing the supplementing mode during the process of ethanol production by Clostridium fermentation of synthesis gas, so that ethanol becomes the main product of fermentation. The fermentation production method provided by the present application has the advantages of high efficiency, high yield, high proportion of ethanol in fermentation products, high biomass, simple process and easy operation, and has a good application prospect in the production of ethanol by synthesis gas fermentation, and provides a new means and method for efficient synthesis of ethanol and single-cell protein by synthesis gas.

The invention belongs to the technical field of catalysts. The invention provides quaternary ammonium base ionic liquid with a structure as shown in a formula (I). The method can be used for synthesis processes of synthesizing methyl ethyl carbonate from dimethyl carbonate and diethyl carbonate and synthesizing methyl ethyl carbonate from dimethyl carbonate and ethanol, and the synthesized methyl ethyl carbonate has excellent purity. Besides, the used quaternary ammonium base ionic liquid has the advantages of being easy to prepare, low in toxicity and renewable, the problems that a traditional sodium alcoholate catalyst is easy to cake and inactivate and difficult to reuse are solved, and the method has important significance for promoting green and efficient production of methyl ethyl carbonate.

The invention belongs to the technical field of ethanol synthesis, and discloses a gas-liquid combined two-step method acetic acid hydrogenation device and process. The device comprises a heating mixer, a reactor, a gas-liquid separator, a liquid phase hydrogenation reaction kettle, a separator and a rectifying tower which are connected in sequence. The process comprises three working sections of acetic acid hydrogenation, acetaldehyde and acetic ether hydrogenation and crude product purification. Acetic acid is hydrogenated under the action of a catalyst to prepare acetaldehyde and acetic ether, in the acetaldehyde and acetic ether hydrogenation section, a reaction mixture in the acetic acid hydrogenation section is directly hydrogenated to prepare ethanol, hydrogen-rich tail gas and fresh hydrogen are mixed and enter the acetic acid hydrogenation section to serve as reaction gas, crude ethanol is rectified and purified, and high-purity ethanol is obtained. According to the invention, continuous production is realized, the hydrogen is subjected to deep reaction, the raw materials have higher conversion rate and yield, and the operation continuity and stability are very high. The corrosion of direct hydrogenation of acetic acid to equipment is reduced, and the defects of high energy consumption and huge equipment scale caused by an ethanol system are avoided.

The invention belongs to the technical field of microorganisms, and discloses a Saccharomyces cerevisiae DG-11 strain with low acetic acid yield, and the preservation number of the Saccharomyces cerevisiae DG-11 strain is CCTCC (China Center for Type Culture Collection) M 20251925. The strain is obtained by combining ARTP mutagenesis with 2-deoxyglucose (2-DG) resistance screening, and has the following characteristics that in simulated fruit juice fermentation, the yield of acetic acid is remarkably reduced compared with that of an original strain RV002; in the metabolic pathway, the activities of pyruvate decarboxylase (Pdc) and acetaldehyde dehydrogenase (Ald) are inhibited, and the activity of ethanol dehydrogenase (Adh) is enhanced, so that carbon metabolism is inclined to ethanol synthesis; meanwhile, the strain has excellent ethanol, high glucose and pH tolerance, and is stable in hereditary character. In addition, the content of acetic esters in the DG-11 fermentation liquor is reduced, and the content of part of ethyl esters and higher alcohols is increased, so that the flower and fruit aroma characteristics are favorably formed. The saccharomyces cerevisiae strain with excellent performance and low acetic acid yield is provided for fruit wine brewing, and the saccharomyces cerevisiae strain has important industrial application value.

An electrolyzer system is provided with a plurality of separator-electrode assemblies (SEA). The SEAs include a negative electrode, a positive electrode, and a porous separator positioned therebetween. The electrodes and the separator, and thus the SEA overall, have a flow-through configuration enabling bulk liquid- and gas-phase reactants and products to flow therethrough. SEAs are positioned serially along a length of the electrolyzer, and thus bulk flow occurs through each SEA cell sequentially, in contrast to "flow-by" architectures where flow occurs in parallel through all cells simultaneously. Liquid- and gas-phase inlets provide the reactants such as H2, CO2, O2, etc. which are electrochemically converted to value products by the SEAs and concentrated for removal by product outlets. These electrolyzer systems generate value products such as H2O2; epoxides; C2+ alcohols, such as ethanol; syngas (CO + H2); etc. even at room temperature while avoiding the disadvantages associated with degradation-prone polymeric ion exchange membranes.

The catalyst in this embodiment of the invention is a catalyst for the synthesis of 1,3-butadiene from ethanol. This catalyst comprises a porous silica support made of crystalline silica, Zn, and Zr, and has a peak pore size (D) of less than 2 nm. 微 ) and peak aperture greater than 2 nm (D 中+大 The multi-peaked pore size distribution.

A catalyst according to an embodiment is a catalyst for synthesizing 1,3-butadiene from ethanol. The catalyst contains a porous silica support made of crystalline silica, Zn, and Zr, and has a multimodal pore size distribution with a peak pore size (Dmicro) of 2 nm or less and a peak pore size (Dmeso+macro) of more than 2 nm.

This utility model discloses a continuously dehydrated ethanol synthesis reaction system, comprising: a gas-liquid reactor, wherein the lower part of the gas-liquid reactor is provided with a first air inlet for inputting fresh raw materials, and the upper part is provided with a first liquid inlet for inputting fresh raw materials; a first air outlet at the top of the gas-liquid reactor is connected to a second air inlet at the lower end of the gas-liquid reactor via a pipeline to form a gas-phase external circulation loop; a first liquid outlet at the bottom of the gas-liquid reactor is connected to the second liquid inlet at the upper part of the gas-liquid reactor via a dehydration device to form a liquid-phase external circulation loop; wherein the dehydration device includes at least two parallel adsorption columns, which allows the dehydration device to alternately perform water adsorption and water desorption operations. By using at least two parallel adsorption columns to alternately adsorb and desorb, continuous production operation can be achieved, and the adsorption / desorption cycle can be dynamically adjusted according to the reactor water outlet rate to optimize adsorption efficiency and improve reaction efficiency.

This invention provides an ethanol synthesis catalyst for synthesizing ethanol from carbon dioxide, comprising at least one support selected from the group consisting of ZrO2, Al2O3, and TiO2, Fe and Cu supported on said support, and Na and / or K supported on said support. In one embodiment, the catalyst containing iron oxide and copper oxide as Fe and Cu supported on the support can be used. Hydrogen is supplied to the catalyst to reduce the iron oxide and copper oxide, and a reaction gas containing CO2 is supplied to the reduced catalyst to obtain ethanol from CO2.

The invention relates to a composition, production method, or synthesis method for obtaining Tetracycline (Chemical formula C22H24N2O8) from bamboo. The raw materials consist of 1) Bamboo, 2) A substance for reacting with bamboo (water / aqueous agent), and 3) Ethyl Alcohol. The synthesis process involves two main steps: reacting the bamboo with the water-based agent, followed by reacting the resulting substance with Ethyl Alcohol.