Method for producing ethanol by glucose fed-batch fermentation based on online ethanol concentration response value monitoring
An ethanol concentration, fed-feed fermentation technology, applied in the field of microbial fermentation, can solve the problems of inability to realize key index parameters, expensive near-infrared instruments, long detection time, etc., to improve ethanol production efficiency and sugar alcohol conversion rate, and alleviate high bottom The inhibitory effect of the concentration of the substance, the effect of reducing the initial glucose concentration
Pending Publication Date: 2021-03-26
EAST CHINA UNIV OF SCI & TECH +1
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Problems solved by technology
However, at present, the control of industrial ethanol fermentation process is still based on manual experience, and the online detection parameters of the process are limited to temperature, pH, etc., so it is impossible to realize the online monitoring of key index parameters, especially the monitoring of product ethanol
[0004] At present, the commonly used method for ethanol detection is liquid chromatography, but there are problems such as off-line, high co
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Embodiment 1
[0078] This embodiment provides a method for producing ethanol by glucose-fed fermentation, the steps of the method comprising:
[0079] ①Seed culture: Pick a single colony from the plate, put it in a 250mL shake flask with 100mL seed medium, and culture it in a shaker at 30°C and 220rpm for 14h to obtain a seed culture solution;
[0080] 2. fermentation culture produces ethanol: with 20% inoculum size, described seed culture solution is inserted in the 5L sealed fermenter that fermentation medium is housed (working volume is 3L), and initial glucose concentration is 100g / L in the fermentation solution, The culture temperature was 30° C., the stirring speed was 150 rpm, and the fermentation culture was carried out.
[0081] During the fermentation and cultivation process, the response value of ethanol concentration in the fermentation liquid is monitored online: the fermentation liquid is drawn from the fermenter to the external bottle through a peristaltic pump, and then the ...
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The invention discloses a method for producing ethanol by glucose fed-batch fermentation based on online ethanol concentration response value monitoring. The method comprises the following steps: a step of conducting seed culture; and a step of producing ethanol by fermentation culture, wherein in the step of producing the ethanol by fermentation culture, the online ethanol concentration responsevalue monitoring is carried, and when the ethanol concentration response value is in a descending trend, glucose feeding is started to be carried out, and fermentation is continued. According to the method disclosed by the invention, the electronic olfactory is introduced into the ethanol fermentation process, and the relationship between the electronic olfactory and the ethanol concentration is established to prove that the electronic olfactory can realize the online monitoring of key indexes and parameters, especially the product ethanol concentration, in the fermentation process, so that glucose is added in stages in the ethanol fermentation process in an online guidance manner, and the inhibition effect on cell growth and ethanol synthesis in the fermentation process is relieved, thuseffectively improving the ethanol production efficiency and the sugar-ethanol conversion rate.
Description
technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for producing ethanol by glucose fed-feed fermentation based on online ethanol concentration response value monitoring. Background technique [0002] With the consumption of fossil fuels and the escalation of environmental pollution and global warming caused by the use of fossil fuels, biofuels are considered to be the most potential renewable biomass energy in order to cope with global climate change and energy security. risks of. Replacing fossil fuels with biofuel ethanol can reduce 90% of car carbon dioxide emissions, thus showing better environmental friendliness, but there are still certain disadvantages in terms of economy. Therefore, the development of efficient fuel ethanol fermentation technology is the key to achieve low-cost production. [0003] Process detection plays an important role in the understanding and regulation of fermentation proc...
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Login to view more IPC IPC(8): C12P7/06C12N1/18C12Q3/00G01N33/00G01N21/31G01N5/04G01N30/88C12R1/865
CPCC12P7/06C12N1/18C12Q3/00G01N33/00G01N21/31G01N5/04G01N30/88G01N2030/8886Y02E50/10
Inventor 田锡炜庄英萍冯瑶陈阳王泽宇王冠王海艺
Owner EAST CHINA UNIV OF SCI & TECH
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