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Method for improving clostridium butyricum growth efficiency

A technology of butyric acid bacteria and efficiency is applied in the field of improving the growth efficiency of butyric acid bacteria, and achieves the effects of good technical effect, promoting oxidation efficiency and improving growth efficiency.

Active Publication Date: 2016-08-17
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of acetaldehyde molecule as a metabolic factor to the new function of anaerobic microorganisms---butyric acid bacteria cultivation process, that is: acetaldehyde, as a metabolic regulation molecule, promotes the activity of alcohol dehydrogenase in the cells of butyric acid bacteria. Reports of new functions that enhance the production efficiency of ethanol, may also alleviate the acidosis effect and further improve the growth efficiency of butyric acid bacteria cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of medium

[0025] Glucose 20 g / L, peptone 30 g / L, yeast powder 20 g / L, dipotassium hydrogen phosphate 0.5 g / L, magnesium sulfate heptahydrate 0.5 g / L, light calcium carbonate 1 g / L, manganese sulfate monohydrate 0.02 g / L, pH7.3; anaerobic tube slant medium needs to add agar, the concentration of agar is 20; pH 7.3. After deoxygenation, it was sterilized by steam at 0.1 MPa for 20 minutes for later use.

[0026] (2) Seed expansion culture and collection of bottled seed cells

[0027] According to the requirements of aseptic and anaerobic operation, the cells were transferred from the freeze-dried tube of Butyricum strain to the newly prepared slant medium of anaerobic tube. After culturing at 37°C for 12 h, according to the requirements of sterile and anaerobic operation, add 10 mL of sterile and anaerobic water to prepare anaerobic tube seed solution.

[0028]30 mL of 120 mL anaerobic bottled culture medium, with 1% (v / v) Butyric acid bacteria ( Clo...

Embodiment 2

[0035] (1) Preparation of medium

[0036] Glucose 16.6 g / L, yeast powder 7.2 g / L, calcium carbonate 4.7 g / L, peptone 25 g / L, dipotassium hydrogen phosphate 0.5 g / L, magnesium sulfate heptahydrate 0.8 g / L, manganese sulfate monohydrate 0.02 g / L, pH7.3; anaerobic tube slant medium needs to be added with agar, the concentration of agar is 20 g / L. After deoxygenation, it was sterilized by steam at 0.1 MPa for 20 minutes for later use.

[0037] (2) Seed expansion culture and collection of bottled seed cells

[0038] According to the requirements of aseptic and anaerobic operation, the cells were transferred from the freeze-dried tube of Butyricum strain to the newly prepared slant medium of anaerobic tube. After incubating at 37°C for 12 h, according to the requirements of sterile and anaerobic operation, add 10 mL sterile and anaerobic water to prepare anaerobic tube seed solution.

[0039] 30 mL of 120 mL anaerobic bottled culture medium, with 1% (v / v) Butyric acid bacteria (...

Embodiment 3

[0046] (1) Preparation of medium: same as Example 2.

[0047] (2) Expanded cultivation of seed solution and collection of seed cells in bottle: same as in Example 2.

[0048] (3) Secondary seed cultivation: same as in Example 2.

[0049] (4) Tertiary fermentation: Except for the addition of regulatory factors, the remaining conditions of the tertiary fermentation are the same as in Example 2.

[0050] Regulatory factor supplementation: At the beginning of the tertiary fermentation culture, acetaldehyde solution (concentration: 0.2 mol / L) was added in a sterile, anaerobic uniform flow until 4 hours before the end of fermentation, and the total concentration of acetaldehyde was 3.0 mmol / L.

[0051] Anaerobic fermentation was carried out for 24 h as above, and the number of cells (counted by colony CFU by Hungate rolling tube counting method) was 6.56×10 8 CFU / mL, increased by 15.1% compared with Example 2; the concentration of ethanol in the fermentation broth was 124 mg / L, i...

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Abstract

The invention discloses a method for improving the clostridium butyricum growth efficiency, and belongs to the field of food fermentation. The invention provides the method for improving the clostridium butyricum growth efficiency by reducing generation of acidic products in the fermentation process and promoting ethanol synthesis, and the method particularly comprises the step that phenylalanine solution feeding is conducted in a sterile and anaerobic mode at constant speed when three stage fermentation of clostridium butyricum is conducted for 0.8 h till 4 h to 6 h before fermentation is completed, wherein the total concentration of acetaldehyde ranges from 1.5 mmol / L to 5.0 mmol / L. According to the method for improving the clostridium butyricum growth efficiency, acetaldehyde molecules serve as metabolic regulation molecules for use; by means of addition of the acetaldehyde molecules, the oxidation efficiency of NADH is promoted, the synthesis efficiency of ethanol is significantly enhanced, the generation efficiency of acetic acid and butyric acid is significantly reduced, the growth efficiency of the clostridium butyricum is further improved, and an excellent technical effect is produced. The method for improving the clostridium butyricum growth efficiency has the advantages that the materials are easy to purchase, the operation controllability is high, and the method is suitable for clostridium butyricum fermentation production.

Description

[0001] technical field [0002] The invention belongs to the field of food fermentation, and in particular relates to a method for improving the growth efficiency of butyric acid bacteria. Background technique [0003] In addition to playing an important role as an important functional microorganism in the process of sauces, pickles, and wine, butyric acid bacteria (Clostridium butyricum, Clostridium butyrium ) as a new probiotic for humans or animals has the functions of adjusting the balance of intestinal flora, promoting the proliferation of beneficial intestinal flora, enhancing immunity, and preventing tumor occurrence. In addition, the probiotics produced by butyric acid bacteria in the intestine, such as B vitamins, vitamin K and other substances, have health care effects on the body. The main metabolite of butyric acid bacteria is butyric acid, which is of great significance to the regeneration and repair of intestinal epithelial tissue. Butyric acid bacteria is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C12R1/145
CPCC12N1/20C12N1/38
Inventor 王志夏会丽陈雄李冬生
Owner HUBEI UNIV OF TECH
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