Methods and uses of a modified cecropin for treating endoparasitic and bacterial infections

A technology of cecropin, endoparasites, applied in biochemical equipment and methods, chemical instruments and methods, botanical equipment and methods, etc.

Active Publication Date: 2014-08-06
SOLARVEST BIOENERGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such channels may only form when cecropin density is high and membrane disruption occurs [Christensen, B.J. et al., 1988; Durell, S.R., et al., 1992]

Method used

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  • Methods and uses of a modified cecropin for treating endoparasitic and bacterial infections
  • Methods and uses of a modified cecropin for treating endoparasitic and bacterial infections
  • Methods and uses of a modified cecropin for treating endoparasitic and bacterial infections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0106] Materials and methods:

[0107] gene synthesis

[0108] The cecropin gene sequence from Calvo et al. (2009) was codon optimized for expression in yeast cells and a hydrophilic tail of 8 amino acids in length was added to it. The nucleic acid and amino acid sequences of the obtained cecropin are shown in Tables 1 and 2, respectively. Insert this gene into the plasmid.

[0109] Plasmid construction

[0110] The cecropin gene was excised from the vector plasmid using appropriate restriction enzymes, purified and placed at the BmtI-MluI site (see figure 1 ) was ligated into the plasmid (Leu2 yeast vector). Clones were verified by further digestion with restriction enzymes specific for the BmtI and MluI loci.

[0111] Cloning and Transformation Strategies

[0112] Transformation into yeast was performed using LiAc using the 'Gietz & Woods' yeast transformation protocol [Geitz RD & R A Woods, 2002]. Transformed clones were tested for the presence of the gene...

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Abstract

The present disclosure provides a modified cecropin protein comprising a cecropin or a variant thereof fused to a hydrophilic tail lacking a C-terminal glycine. The present disclosure also provides pharmaceutical compositions, methods and uses of the modified cecropin protein or nucleic acid for treating or preventing endoparasites, such as Plasmodium, and bacteria.

Description

[0001] related application [0002] This non-provisional application claims priority from US Provisional Application No. 61 / 507,366, filed July 13, 2011, which is hereby incorporated by reference in its entirety. field of invention [0003] The present disclosure relates to modified cecropin antimicrobial peptides and their methods and uses for inhibiting endoparasites or bacteria and for treating or preventing endoparasites or bacterial infections. Background of the invention [0004] Malaria is a mosquito-borne disease and despite years of research, remains a major global health problem causing disease and death, disproportionately affecting developing countries. The World Health Organization estimates the worldwide incidence of malaria to be approximately 300-500 million clinical cases per year, with at least 1 million deaths. The majority of these individuals were young children [WHO malaria report 2008, Snow et al., 2005, Guerra et al., 2008 and Hay Si et al., 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12A61K38/16A61P31/04A61P33/00A61P33/06C07K14/435
CPCC07K14/43563A61K38/00C07K2319/00A61P31/04A61P33/00A61P33/06Y02A50/30
Inventor S.乔扈里M.T.霍尔恩
Owner SOLARVEST BIOENERGY
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