Housefly cecropin-human lysozyme fusion protein, and preparation method and application thereof

A technology of housefly cecropin and human lysozyme, which is applied in the field of preparation of genetically engineered antibacterial proteins, can solve the problems of not wide antibacterial spectrum, high cost, and inconspicuous antibacterial effect, so as to improve expression efficiency and reduce the probability of tolerance , the effect of low production cost
CN101817883AInactive Publication Date: 2010-09-01GUANGDONG PHARMA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGDONG PHARMA UNIV
Publication Date
2010-09-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a housefly cecropin-human lysozyme fusion protein, and a preparation method and application thereof. A sequence of the housefly cecropin-human lysozyme fusion protein of the invention is shown by SEQ ID No.1. The housefly cecropin-human lysozyme fusion protein comprises a mature peptide sequence of housefly cecropin, a polypeptide linker sequence and the mature peptide sequence of human lysozyme. The housefly cecropin-human lysozyme fusion protein is prepared by the following steps of: according to the gene sequences of the housefly cecropin and the human lysozyme, designing a synthetic primer with esherichia coli preferred codons; performing PCR amplification by using a splicing overlap extension method to obtain the gene sequence of the fusion protein so as to construct recombinant prokaryotic expression plasmids; transferring the recombinant prokaryotic expression plasmids into host cells to obtain engineering bacteria expressing the fusion protein; and performing fermentation culture, separation and purification to obtain the housefly cecropin-human lysozyme fusion protein of the invention. The fusion protein of the invention has the characteristics of the housefly cecropin and the human lysozyme per se, has relatively more remarkable antibacterial activity and antibiotic activity, can be used for preparing antibacterials or bacteriostatic medicaments, and has wide application prospect.
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Description

technical field

[0001] The invention relates to the field of preparation of genetically engineered antibacterial proteins, in particular to a fusion protein of housefly cecropin-human lysozyme and its preparation method and application. Background technique

[0002] Since the discovery of penicillin, various natural, semi-synthetic and synthetic compounds have been widely and successfully applied to resist various microbial infections. However, with the change of environment and the adaptive evolution of microorganisms, most pathogenic microorganisms are Resistance to traditional antibiotics has become a serious problem affecting public health (Bonomo RA. Multiple antibiotic-resistant bacteria in long-term-care facilities: Anemerging problem in the practice of infectious diseases[J]. Clin Infect Dis, 2000, 31 : 1414-1422.; Murinda SE, Ebner PD, Nguyen LT, et al. Antimicrobial resistance and class 1 integrons in pathogenic Escherichia coli from dairy farms[J]. Foodborne Patho...

Claims

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