Antimicrobial peptide and prokaryotic expression method and application thereof

A prokaryotic expression and antimicrobial peptide technology, applied in the field of genetic engineering, can solve the problems of low potency, limited sources of natural antibiotics, high cost of artificial synthesis, etc., and achieve the effect of simple method and obvious antibacterial effect in vitro

Active Publication Date: 2019-01-25
ZHEJIANG UNIV
View PDF10 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural antibiotics also have disadvantages such as limited sources, low potency, and long polypeptide sequences leading to high artificial synthesis costs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antimicrobial peptide and prokaryotic expression method and application thereof
  • Antimicrobial peptide and prokaryotic expression method and application thereof
  • Antimicrobial peptide and prokaryotic expression method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] The following specific examples are further descriptions of the methods and technical solutions provided by the present invention, but should not be construed as limiting the present invention.

[0033] One, the material and source that adopt in the specific embodiment of the present invention are as follows:

[0034] 1 strains and plasmids

[0035] DH5α Chemically Competent Cell (Beijing Qingke Xinye Biotechnology Co., Ltd.); BL21(DE3) Chemically Competent Cell (Shanghai Weidi Biotechnology Co., Ltd.); pMD19-T Vector (TaKaRa Company); pET-28a(+) vector, E .Coli and Staphylococcus aureus were preserved in our laboratory.

[0036] 2 reagents

[0037] High-fidelity enzyme PrimeSTAR HS DNA Polymerase, Taq enzyme, agarose gel electrophoresis 10×LoadingBuffer, DL1000DNA Marker, restriction endonuclease EcoRI and BamHI, plasmid ligation DNA LigationKit, nickel column His60Ni Gravity Columns used for protein purification are all from TaKaRa Company Products; AxyPrep Plasmid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention discloses an antimicrobial peptide and a prokaryotic expression method and application thereof. The invention adopts prokaryotic expression vector to construct the recombinant vector ofthe encoding gene of the antibacterial peptide cecropin-BM, and prokaryotic expression of the antibacterial peptide cecropin-BM is achieved. The method is simple and easy, the purified recombinant antibacterial peptide has obvious antibacterial effect in vitro without any treatment.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an antibacterial peptide and its prokaryotic expression method and application. Background technique [0002] Antimicrobial peptide (antimicrobial peptide) is a small molecular polypeptide naturally present in almost all organisms, and is an important part of the natural immunity of organisms. Viruses also have a killing effect. Different from conventional antibiotics, antimicrobial peptides are not easy to cause bacterial drug resistance, and also have a killing effect on antibiotic-resistant strains, showing great potential in the field of new antibacterial drug development. However, natural antibiotics also have disadvantages such as limited sources, low potency, and long polypeptide sequences leading to high artificial synthesis costs. Therefore, it is of great significance to develop and prepare a new generation of antimicrobial peptides that can ov...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435C12N15/70
CPCC07K14/43586C12N15/70
Inventor 谭勋邵冯金应易恬
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products