Microbial pesticide for preventing and controlling prodenia litura

A microbial pesticide, Spodoptera litura technology, applied in biocides, animal repellents, plant growth regulators, etc., can solve the problems of yield and quality loss, pest resistance, drug resistance enhancement, animal natural enemies killing and other problems

Inactive Publication Date: 2012-10-17
HUNAN TOBACCO CHENZHOU +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is an important pest of my country's grain, cotton, tobacco, vegetables and other crops, and is also one of the important pests in many countries and regions in the world. The harm of Spodoptera litura causes serious yield and quality loss every year

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A microbial pesticide for preventing Spodoptera litura is characterized in that 1 part of virus stabilizer and 1 part of virus compound are stirred and dissolved, and the pH value is adjusted to 6.8 with dilute sulfuric acid solution; the above virus stabilizer is obtained by weight ratio , virus stock solution: glycerol: water=1:1.2:2.3 parts by weight is equipped; above-mentioned virus compound is by weight ratio, and virus stock solution: 5% emamectin benzoate=1:1.2 parts by weight is equipped;

[0015] The virus stock solution is to use the production strain of Spodoptera litura nuclear polyhedrosis virus, Spodoptera litura pests, and fresh tobacco leaves as the main virus propagation materials, and obtain the virus stock solution through breeding pests, virus inoculation, collecting diseased and dead insects, and pressing and filtering; The extraction process is as follows:

[0016] ① Virus reproduction: use the feeding method to reproduce. The first batch of feed ...

Embodiment 2

[0026] A microbial pesticide for preventing Spodoptera litura is characterized in that 1 part of virus stabilizer and 1 part of virus compound are stirred and dissolved, and the pH value is adjusted to 6.8 with dilute sulfuric acid solution; the above virus stabilizer is obtained by weight ratio , virus stock solution: glycerin: water=1:1.67:3.3 parts by weight is equipped; above-mentioned virus compound agent is by weight ratio, and virus stock solution: 5% emamectin benzoate=1:1.25 parts by weight is equipped;

[0027] The virus stock solution is to use the production strain of Spodoptera litura nuclear polyhedrosis virus, Spodoptera litura pests, and fresh tobacco leaves as the main virus propagation materials, and obtain the virus stock solution through breeding pests, virus inoculation, collecting diseased and dead insects, and pressing and filtering; The extraction process is as follows:

[0028] ① Virus reproduction: use the feeding method to reproduce. The first batch ...

Embodiment 3

[0032] A microbial pesticide for preventing Spodoptera litura is characterized in that 1 part of virus stabilizer and 1 part of virus compound are stirred and dissolved, and the pH value is adjusted to 6.8 with dilute sulfuric acid solution; the above virus stabilizer is obtained by weight ratio , Virus stock solution: glycerin: water=1:1.38:2.3 parts by weight is equipped; above-mentioned virus compound agent is by weight ratio, and virus stock solution: 5% emamectin benzoate=1:1.1 parts by weight is equipped,

[0033] The virus stock solution is to use the production strain of Spodoptera litura nuclear polyhedrosis virus, Spodoptera litura pests, and fresh tobacco leaves as the main virus propagation materials, and obtain the virus stock solution through breeding pests, virus inoculation, collecting diseased and dead insects, and pressing and filtering; The extraction process is as follows:

[0034] ① Virus reproduction: use the feeding method to reproduce. The first batch o...

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PUM

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Abstract

The invention discloses a microbial pesticide for preventing and controlling prodenia litura. In the microbial pesticide, the prodenia litura larva, prodenia litura nuclear polyhedrosis virus production strain stock solution and fresh tobacco are used as major raw materials, and virus reproduction is performed. The microbial pesticide is prepared mainly by the following steps of: breeding the larva, inoculating the viruses, collecting the larva died of illness and performing crude extraction and filtration to obtain the virus stock solution; mixing the virus stock solution, glycerin and water at a weight ratio of 1:(1.2-4):(2.3-6) to prepare a virus stabilizer; preparing a virus mixture from the virus stock solution and 5% emamectin benzoate at a weight ratio of 1:(1.1-1.25); mixing and dissolving the virus stabilizer and the virus mixture; and adjusting the pH value to 6.8 with dilute sulphuric acid solution to obtain the microbial pesticide. The microbial pesticide disclosed by the invention is used for preventing and controlling the prodenia litura pests on crops such as vegetables, tobacco and the like, has high efficiency and environmental protection effect, and improves the quality and yield of the tobacco.

Description

technical field [0001] The invention belongs to the field of biopesticides and applications thereof, and relates to a microbial pesticide for controlling Spodoptera litura. Background technique [0002] Biopesticide refers to a type of pesticide preparation that uses living organisms or their metabolites to control pests, germs, weeds, nematodes, rodents and other harmful organisms. Biological pesticides include microbial-derived pesticides, plant-derived pesticides, animal-derived pesticides and genetically modified Four categories of biopesticides. Microbial insecticides are an important part of biopesticides, which are a class of insecticides made from microbial groups whose metabolites or microorganisms themselves have lethal effects on host insects or are pathogenic. Spodoptera litura belongs to Lepidoptera Noctuidae and is an omnivorous insect. It harms more than 290 species of plants in 99 families, of which more than 90 species are the main ones. It is an important...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N43/90A01P7/04
CPCY02A50/30
Inventor 郑雄志周志成曾维爱李小一李宏光黄艳宁肖艳松邓正平黄长永罗经仁余金龙李晓燕刘永能周玲红李丽娟
Owner HUNAN TOBACCO CHENZHOU
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