Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rapid acquisition method of lepidopterous larvae midgut

A lepidopteran larva and acquisition method technology, applied in the field of insect anatomy, can solve the problems of cumbersome operation and low dissection efficiency

Inactive Publication Date: 2017-08-18
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is relatively cumbersome to operate, and the efficiency of dissection is low, and it is only suitable for larvae with relatively large age and body size (4-5 instar larvae)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid acquisition method of lepidopterous larvae midgut
  • Rapid acquisition method of lepidopterous larvae midgut
  • Rapid acquisition method of lepidopterous larvae midgut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take dissecting the midgut of the cotton bollworm as an example. Select the mid-5th instar cotton bollworm. Before dissection, the dissection tools were sterilized with 75% absolute ethanol. The whole operation process was in a sterile state, and the instruments were aseptically treated before use. The specific implementation steps are as follows:

[0025] Place the cotton bollworm without vital signs on the sterile operating table, and use surgical scissors to cut off the space between the third abdominal segment and the sixth abdominal segment. Such as figure 1 . Carefully pull the midgut out of the snipped abdominal segment. Such as figure 2 . The pulled out midgut was placed in pre-cooled saline (0.7%), the food residue wrapped by the peritrophic membrane was separated from the midgut, and washed. Such as image 3 . The dissected midgut tissue was placed on filter paper to blot dry, quickly put into a sterile EP tube, quick-frozen with liquid nitrogen, and...

Embodiment 2

[0027] Taking the anatomy of the midgut of the 5th instar midgut as an example, compare the midgut obtained by this method and the midgut total protease and glutathione-S-transferase (GST) obtained by the traditional dissection method mentioned in the background technology of the application respectively. , Carboxylesterase (CarEs) activity. Each group of 5 larvae was repeated three times. Add 1.2 mL of 0.1 mol / L phosphate buffer solution of pH 7.6 to the midgut obtained by the above two different methods, and homogenize in ice bath. The homogenate was centrifuged at 12000g, 4°C for 15min, and the supernatant was taken as the crude enzyme solution to be tested. Glutathione-S-transferase (GST), carboxylesterase (CarEs), and midgut total protease activities were determined by reference to relevant literature. The results are shown in Table 1 below.

[0028] Table 1

[0029]

[0030] The results showed that there was no significant difference in the total protease activity...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a rapid acquisition method of a lepidopterous larvae midgut. The rapid acquisition method of the lepidopterous larvae midgut belongs to the technical field of entomotomy, and comprises the steps of cutting off a third uromere to a sixth uromere of a lepidoptera insect with absent vital signs, pulling the midgut out from the cut-off uromeres, putting the midgut in normal saline, separating food debris encapsulated by a peritrophic membrane from the midgut, and washing, thus obtaining the lepidopterous larvae midgut. According to the method provided by the invention, the complete midgut tissue can be obtained, the whole operation process is short, the time can be saved by 50 percent compared with a traditional operation method, and the method is applicable to three-year-old and more than three-year-old lepidopterous larvae.

Description

technical field [0001] The invention relates to the technical field of insect anatomy, in particular to a method for rapidly obtaining the midgut of Lepidoptera larvae. Background technique [0002] With the long-term and extensive use of chemical pesticides, many pests have developed resistance to varying degrees. The increase of pest resistance not only leads to the increase of agricultural production cost, but also has a huge impact on the environment and public health. [0003] Insect midgut is the main site for secreting digestive enzymes, digesting food and absorbing nutrients. Generally, the midgut of insects is tubular, with the anterior end next to the forestomach, and the posterior end is bounded by the Malpighian tube insertion and the hindgut. Its structure from inside to outside is: peritrophic membrane, intestinal wall cell layer, basement membrane, circular muscle, longitudinal muscle and peritoneal membrane. The peritrophic membrane is secreted by the midg...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/28G01N1/34G01N1/42
CPCG01N1/286G01N1/34G01N1/42G01N2001/2873
Inventor 崔金杰楚博张帅雒珺瑜吕丽敏王丽
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com