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Simple method for rapidly screening substances effective to insects

A technology of insects and substances, applied in the field of pesticide science, can solve the problems of difficult quantitative analysis, increased workload and labor costs, poor repeatability, etc.

Inactive Publication Date: 2017-01-04
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The shortcomings of these methods are: relatively extensive, quantitative analysis is difficult, repeatability is relatively poor, greatly affected by environmental factors, and the effect cannot be guaranteed. It is mainly used to judge whether the compound has insecticidal activity and initially compare its activity. , mostly used for routine screening research, and some require special instruments such as sprayers, settling towers, etc., which increases the workload and labor costs

Method used

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  • Simple method for rapidly screening substances effective to insects
  • Simple method for rapidly screening substances effective to insects
  • Simple method for rapidly screening substances effective to insects

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, using BT crystal protein as the test of small molecular compound

[0042] (1) 0.01 g of BT crystal protein was weighed with an electronic balance, serially diluted with sterilized water to a solution with a concentration of 10 μg / ml, and placed on ice for later use.

[0043] (2) Select 5th instar 2-day-old silkworm larvae with relatively consistent size, place them on ice for 20 minutes, and perform anesthesia treatment.

[0044] (3) For the tested silkworm, use 70% ethanol to carry out surface disinfection on the part to be injected, and dry it slightly, and then use a micro-injector to inject BT solution into the midgut of the silkworm chest section 2-3 side, as shown in Fig. figure 1 , each silkworm was injected with 10 μl, and the control was injected with 10 μl of sterilized water. After the injection, they were frozen on ice for 5 minutes, and then the silkworm larvae were taken out and raised normally. After 24 hours, the number of dead silkworms wa...

Embodiment 2

[0054] Embodiment 2, compound screening test

[0055] (1) Set up test group and control group:

[0056] Test group: Dissolve the small molecular compound to be detected in a suitable solvent, prepare a mother solution, and dilute it to a series of test concentrations, and keep it sealed for future use.

[0057] Control group (control solution): the same solvent as the test group without the test compound.

[0058] (2) Select 5th instar 2-day-old silkworm larvae with relatively consistent size, place them on ice for 20 minutes, and perform anesthesia treatment.

[0059] (3) For the silkworm to be tested, use 70% ethanol to disinfect the surface of the site to be injected, and dry it slightly, then fill the micro-syringe with a solution of the test compound or a control solution, and inject it on the side of the 2-3 section of the silkworm's chest. For the midgut injection, 10 μl of each silkworm was injected, and 10 μl of sterilized water was injected as a control. After the ...

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Abstract

The invention relates to a simple method for rapidly screening substances effective to insects. According to the method, the insects are taken as a target, candidate substances are applied to midguts of the insects, changes of the insects are observed, and the biological activity of the candidate substances for the insects is determined. The method is low in cost, good in repeatability and high in accuracy, special instruments are not required, and great convenience is provided for rapid, accurate and efficient screening of bioactive substances effective to the insects.

Description

technical field [0001] The invention belongs to the field of pesticide science, and more specifically, the invention relates to a simple and convenient method for rapidly screening substances effective to insects. Background technique [0002] Agriculture is the foundation of China's national economy. As the population continues to increase, the quality of arable land continues to decrease, food problems and agricultural development problems are becoming increasingly prominent, and the importance of agricultural economy is increasing day by day. In order to ensure crop yields, solve food problems, and maintain social stability, pesticides are inevitably used in agricultural production. Pesticides play an important role in preventing and controlling crop diseases and insect pests, ensuring the normal growth of crops, increasing the yield per unit area, and maintaining social stability. [0003] While pesticides play an important role in ensuring the yield and quality of crop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 黄武仁凌尔军吴凯
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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